We narrowed to 12,205 results for: 112

1. pQCXIZ GFP PODXL FKBP 4K>R

   Plasmid

   #202421

   Purpose

   Expression of ubiquitination-deficient GFP-tagged PODXL with FKBP-rapamycin binding (FRB) dimerization domain

   Depositor

   David Bryant

   Article

   Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

   Insert

   PODXL (PODXL Human)
   

   Use

   Lentiviral

   Tags

   FKBP and GFP

   Mutation

   K431/525/526/547R

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTT5-hSDC1-2

   Plasmid

   #52378

   Purpose

   expresses human Syndecan-1 GAGAL replaced with GADEV of SDC2 in HEK293-EBNA1 (293E) suspension culture.

   Depositor

   Gordon Laurie

   Article

   Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1

   Insert

   SDC1 (SDC1 Human)
   

   Expression

   Mammalian

   Mutation

   GAGAL of syndecan-1 replaced with of GADED of SDC…

   Promoter

   CMV

   Available Since

   Dec. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

3. JNK2 WT O/E (MAPK9)-pcw107-V5

   Plasmid

   #64617

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   David Sabatini, Kris Wood

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   MAPK9 (transcript variant JNK2-a2) (MAPK9 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Mutation

   none

   Promoter

   PGK

   Available Since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pOTTC1784 - pAAV SYN1 hChR2(H134R)-V5-ER-LBD (ChRERa)

   Plasmid

   #201820

   Purpose

   An adeno-associated viral vector expressing channelrhodopsin-2 fused to a V5 epitope and the ligand-binding domain of estrogen receptor (ChRERa) from a synapsin promoter, for use in PET imaging

   Depositor

   Christopher Richie

   Article

   Bonaventura et al Sci Transl Med. 2023 Jul 26;15(706):eadd

   Insert

   hChR2(H134R)-V5-ER-LBD (ChRERa)
   

   Use

   AAV

   Promoter

   SYN1

   Available Since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. FLAG-MKK6 (S207E,T211E)-pcw107

   Plasmid

   #64625

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   David Sabatini, Kris Wood

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   MAP2K6 (MAP2K6 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Mutation

   S207E,T211E

   Promoter

   PGK

   Available Since

   June 10, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA3-sACE2v6-Fc(IgA1)

   Plasmid

   #145161

   Purpose

   Mammalian expression plasmid for soluble ACE2-Fc fusion (IgA1) high affinity variant 6

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Angiotensin-converting enzyme 2 (ACE2 Human)
   

   Tags

   Fc region of human IgA1

   Expression

   Mammalian

   Mutation

   Extracellular, soluble protease domain (a.a. M1-D…

   Promoter

   CMV

   Available Since

   April 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. myr-FLAG-MEK5-pcw107

   Plasmid

   #64620

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   David Sabatini, Kris Wood

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   MAP2K5 (MAP2K5 Human)
   

   Use

   Lentiviral

   Tags

   Flag and myristoylated

   Promoter

   PGK

   Available Since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pQCXIN- myc ULK1 K46I

   Plasmid

   #27627

   Depositor

   Reuben Shaw

   Article

   Egan et al Science. 2011 Jan 28;331(6016):456-61. d

   Insert

   mouse myc ULK1 K46I (Myc Mouse)
   

   Use

   Retroviral

   Tags

   myc

   Mutation

   Initial M from ULK1 removed. Silent mutation at A…

   Available Since

   April 15, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. px335 Mettl14 sgRNA #1

   Plasmid

   #61515

   Purpose

   encodes sgRNA sequence for targeting mouse Mettl14 locus (Cas9-Nickase strategy)

   Depositor

   Jacob Hanna

   Article

   Geula et al Science. 2015 Feb 27;347(6225):1002-6. d

   Insert

   gcggcagctcctagctcagc
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Jan. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pαH-S-GSAS-B.1.351.v2

    Plasmid

    #173786

    Purpose

    Mammalian expression of SARS-CoV-2 Spike protein South African variant version 2

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Science. 2021 Jun 24. pii: science.abi62

    Insert

    Spike (S-GSAS-B.1.351 variant version 2) (S Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Available Since

    Aug. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pIG-738pUC19-tNGFR-P2A-Kindlin-3

    Plasmid

    #186092

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    KINDLIN3 (FERMT3 Human)
    

    Use

    CRISPR

    Mutation

    WT with tNGFR sequence

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pαH-S-GSAS-B.1.351.v6

    Plasmid

    #173791

    Purpose

    Mammalian expression of SARS-CoV-2 Spike protein South African variant version 6

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Science. 2021 Jun 24. pii: science.abi62

    Insert

    Spike (S-GSAS-B.1.351 variant version 6) (S Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-T7-ABE8e-SpCas9-HF1-P2A-EGFP (LM422)

    Plasmid

    #197505

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-HF1(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8e-nSpCas9-HF1-BPNLS-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations in TadA and nSpCas9-HF1(D10A/N497…

    Promoter

    CMV and T7

    Available Since

    March 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGL3E-hGPR56 e1m promoter normal

    Plasmid

    #52298

    Purpose

    Reporter for normal human GPR56 exon 1m promoter activity. Human GPR56 e1m promoter (chr16:56,230,630-56,230,943 (hg18)) was inserted into pGL3E.

    Depositor

    Christopher A Walsh

    Article

    Bae et al Science. 2014 Feb 14;343(6172):764-8. do

    Insert

    ADGRG1 adhesion G protein-coupled receptor G1 (ADGRG1 Human)
    

    Use

    Luciferase

    Available Since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. shERK2b-mlpx-neo

    Plasmid

    #65231

    Purpose

    encodes a shRNA against ERK2

    Depositor

    Gerardo Ferbeyre

    Article

    Deschenes-Simard et al Genes Dev. 2013 Apr 15;27(8):900-15. doi

    Insert

    shRNA against ERK2 (MAPK1 Human)
    

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. FLAG-YAP2 (8SA)-pcw107

    Plasmid

    #64637

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    David Sabatini, Kris Wood

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    YAP2 (transcript variant 3) (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Mutation

    8SA*

    Promoter

    PGK

    Available Since

    Dec. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

17. PX458-gR2A_ISO

    Plasmid

    #124808

    Purpose

    Vector for expression Cas9 with gRNA specific for rat IgG2a heavy chain locus. Use in combination with pHybr_r2a> plasmids to convert expression of rat IgG2a hybridomas to isotype of choice.

    Depositor

    Ferenc Scheeren, Martijn Verdoes

    Article

    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

    Insert

    hSPCas9
    

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Available Since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR221 - myc ULK1 k46I

    Plasmid

    #27624

    Depositor

    Reuben Shaw

    Article

    Egan et al Science. 2011 Jan 28;331(6016):456-61. d

    Insert

    mouse myc ULK1 K46I (Myc Mouse)
    

    Use

    Gateway donr vector

    Tags

    myc

    Mutation

    Initial M from ULK1 removed. Silent mutation at A…

    Available Since

    Feb. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

19. LCV2_PSMD1_sgRNA_1

    Plasmid

    #155091

    Purpose

    lentiviral plasmid expressing Cas9 and gRNA targeting PSMD1 (core essential gene)

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    PSMD1_sgRNA_1 (PSMD1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6 promoter

    Available Since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pαH-S-GSAS-B.1.351.v3

    Plasmid

    #173787

    Purpose

    Mammalian expression of SARS-CoV-2 Spike protein South African variant version 3

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Science. 2021 Jun 24. pii: science.abi62

    Insert

    Spike (S-GSAS-B.1.351 variant version 3) (S Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Available Since

    Aug. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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