We narrowed to 13,131 results for: LIC

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pMRMTK-clo3

Plasmid

#203921

Purpose

Cloning plasmid containing the ColE1 origin insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

ColE1 Origin of Replication

Expression

Plant

Promoter

N/A

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo3'

Plasmid

#203924

Purpose

Cloning plasmid containing the ColE1 origin insert with an ampicillin resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

ColE1 Origin of Replication

Expression

Plant

Promoter

N/A

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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cytoDCIP

Plasmid

#193861

Purpose

Encodes mCherry-GFP1-10 in pDGB1-aR backbone for plant expression. Requires pSOUP for replication in agrobacterium.

Depositor

Markita Landry

Article

Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

Insert

mCherry-GFP1-10

Expression

Plant

Promoter

35S

Available Since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pXC3cIfm

Plasmid

#193750

Purpose

Expresses frame-shifted CI under the control of PLUX promoter, p15A origin of replication, Chloramphenicol selection

Depositor

Sangram Bagh

Article

Sarkar et al Chem Sci. 2021 Nov 9;12(48):15821-15832.

Insert

Frame-shifted CI

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLUX

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pLT237

Plasmid

#174300

Purpose

Replicating plasmid with homology region for introducing PolCC669Y mutation. Confers thiamphenicol resistance.

Depositor

Lee Lynd

Article

Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

Insert

PolC homology arm

Expression

Bacterial

Mutation

changed Cysteine 669 to Tyrosine

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-6-ELuc-9

Plasmid

#197736

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

Emerald/Enhanced Click Beetle Luciferase (ELuc) (tomato codon-optimized)

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pPINA7A2mTFP1(F)

Plasmid

#193774

Purpose

Expresses mTFP1 under the control of PINA7 synthetic promoter, ColE1 origin of replication, Ampicillin selection

Depositor

Sangram Bagh

Article

Sarkar et al Chem Sci. 2021 Nov 9;12(48):15821-15832.

Insert

mTFP1

Use

Synthetic Biology

Expression

Bacterial

Promoter

PINA7 synthetic promoter carrying binding sites f…

Available Since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCRISPR3_BC2

Plasmid

#191857

Purpose

Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: ATCATG) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

Depositor

Kimberly Reynolds

Article

Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

Type

Empty backbone

Use

CRISPR

Available Since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pPB EF1a CCR6-hLOV-TEVcs-Gal4

Plasmid

#173120

Purpose

Expresses CCR6 hLOV receiver construct in DIPG

Depositor

Alice Ting

Article

Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

Insert

CCR6-hLOV-TEVcs-Gal4

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV CMV CCR6-eLOV-TEVcs-Gal4

Plasmid

#173113

Purpose

Expresses CCR6 receiver construct in mammalian cells

Depositor

Alice Ting

Article

Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

Insert

CCR6-eLOV-TEVcs-tTA

Use

AAV

Promoter

CMV

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPR3_BC6

Plasmid

#191861

Purpose

Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: CCTAGT) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

Depositor

Kimberly Reynolds

Article

Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

Type

Empty backbone

Use

CRISPR

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPR3_BC5

Plasmid

#191860

Purpose

Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: AGTCTA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

Depositor

Kimberly Reynolds

Article

Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

Type

Empty backbone

Use

CRISPR

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPR3_BC4

Plasmid

#191859

Purpose

Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: GTATGA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

Depositor

Kimberly Reynolds

Article

Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

Type

Empty backbone

Use

CRISPR

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPR3_BC3

Plasmid

#191858

Purpose

Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: GCATGG) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

Depositor

Kimberly Reynolds

Article

Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

Type

Empty backbone

Use

CRISPR

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPR3_BC1

Plasmid

#191856

Purpose

Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: CTTTCA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

Depositor

Kimberly Reynolds

Article

Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

Type

Empty backbone

Use

CRISPR

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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T7-TetO-GFP_ColE1

Plasmid

#173193

Purpose

Expresses GFP under the control of a T7-TetO promoter. Expression can be repressed via TetR, and repression can be relieved via addition of tetracycline. Contains the ColE1 origin of replication.

Depositor

Tara Deans

Article

MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

Insert

T7-TetO

Tags

ssrA degradation tag (DAS+4)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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QUAS-0-T7-TetO-GFP_ColE1

Plasmid

#171662

Purpose

QUAS directly upstream of a T7 promoter with a TetO operator. Expresses GFP in BL21(DE3) E. coli. Contains the ColE1 origin of replication.

Depositor

Tara Deans

Article

MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

Insert

QUAS-T7-TetO-GFP ssrA-T7 Stop

Tags

ssrA degradation tag (DAS+4)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pVH228-Tier1-OVanO2-PCMVmin2-SEAP

Plasmid

#169564

Purpose

Tier-1 vector encoding PVanO2-driven SEAP expression (OVanO2-PCMVmin-2-SEAP-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

vanillic acid-controlled SEAP production

Expression

Mammalian

Promoter

VanO2-PCMVmin

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pDisplay-V5-Nxph1

Plasmid

#184336

Purpose

Expression of V5-tagged mouse neurexophilin-1

Depositor

Peter Scheiffele

Article

Hauser et al Neuron. 2022 May 8. pii: S0896-6273(22)0

Insert

neurexophilin-1 (Nxph1 Mouse)

Tags

V5-tag

Expression

Mammalian

Promoter

CMV

Available Since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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pSEM262 - pDESTR4-R3 mosTI hygroR target

Plasmid

#159840

Purpose

Single copy or array insertion by MosTI using split hygroR selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

pDESTR4-R3 mosTI hygroR target

Expression

Worm

Mutation

Not applicable

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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