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pDONR223_ETV1_WT

Plasmid

#82209

Purpose

Gateway Donor vector containing ETV1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ETV1 (ETV1 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_NANOG_WT

Plasmid

#82148

Purpose

Gateway Donor vector containing NANOG , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

NANOG (NANOG Human)

Use

Gateway Cloning

Mutation

K82N

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pRF-HlyIIR

Plasmid

#49361

Depositor

Christopher Voigt

Article

Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

Insert

HlyIIR repressor

Use

Synthetic Biology

Expression

Bacterial

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pCoofy6

Plasmid

#43990

Depositor

Sabine Suppmann

Article

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Tags

His6-Sumo3

Expression

Bacterial

Promoter

T7

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pHEN-CoV2-nanobody-G6

Plasmid

#194589

Purpose

Bacterial expression of G6 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

Depositor

Gerald McInerney

Article

Hanke et al Sci Adv. 2022 Mar 25;8(12):eabm0220. doi

Insert

G6 (S )

Expression

Bacterial

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pC28-LIC-sfGFP-His

Plasmid

#165479

Purpose

pET based vector for protein expression in E.coli for targets fused with C-terminal superfolder GFP and His tag

Depositor

Renato Bruni

Article

Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

Type

Empty backbone

Tags

TEV-GFP-6xHis

Expression

Bacterial

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pME-Lifeact

Plasmid

#109545

Purpose

Multisite gateway vector for 5' tagging with the actin binding motif Lifeact

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

actin binding motif Lifeact

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-SYNZIP17

Plasmid

#80671

Purpose

Encodes the synthetic coiled coil peptide SYNZIP17

Depositor

Amy Keating

Article

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP17

Use

Gateway Cloning

Available Since

Nov. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR221-CAG.Pro

Plasmid

#79490

Purpose

MultiSite Gateway entry clones, first fragment, (attL1, attR5) CAG promoter

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

CAG Promoter

Use

Gateway Cloning

Promoter

CAG Promoter

Available Since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CCNG1_WT

Plasmid

#81767

Purpose

Gateway Donor vector containing CCNG1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CCNG1 (CCNG1 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_AKR1B1_WT

Plasmid

#82928

Purpose

Gateway Donor vector containing AKR1B1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

AKR1B1 (AKR1B1 Human)

Use

Gateway Cloning

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_HIF1AN_WT

Plasmid

#82256

Purpose

Gateway Donor vector containing HIF1AN , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

HIF1AN (HIF1AN Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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R777-E359 Hs.YAP1

Plasmid

#70643

Purpose

Gateway ORF clone of human YAP1 [NM_001130145.2] with stop codon (for native or N-terminal fusions)

Depositor

Dominic Esposito

Insert

YAP1 (YAP1 Human)

Use

Gateway Cloning

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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R777-E090 Hs.FGFR4-nostop

Plasmid

#70374

Purpose

Gateway ORF clone of human FGFR4 [NM_002011.4] without stop codon (for C-terminal fusions)

Depositor

Dominic Esposito

Insert

FGFR4 (FGFR4 Human)

Use

Gateway Cloning

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pLux-LmrA

Plasmid

#49375

Depositor

Christopher Voigt

Article

Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

Insert

LmrA repressor

Use

Synthetic Biology

Expression

Bacterial

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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PEW-pEF1A-inactEGFP

Plasmid

#212009

Purpose

Cleavable backbone for constitutive expression of TCR alpha and beta chains through lentiviral transduction

Depositor

Catherine Wu

Article

Afeyan et al Nat Protoc. 2024 Nov 8. doi: 10.1038/s41

Insert

Inactivated eGFP

Use

Lentiviral

Expression

Mammalian

Promoter

pEF1a

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR223-BALF4

Plasmid

#195835

Purpose

Gateway Entry Clone of Epstein-Barr virus BALF4

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BALF4 (BALF4 Epstein-Barr virus)

Use

Gateway Cloning

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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HT109_pAAV_hSyn-DiO-SomQuasAr6a_EGFP

Plasmid

#190878

Purpose

Cre-on expression of soma-targeted QuasAr6a under an neuronal promoter

Depositor

Adam Cohen

Article

Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

Insert

somQuasAr6a_EGFP

Use

AAV

Tags

EGFP

Promoter

hSyn

Available Since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

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HT028_FCMV_CheRiff_EGFP

Plasmid

#178821

Purpose

Expresses a blue-light activated cationic channelrhodopsin CheRiff carrying an EGFP tag

Depositor

Adam Cohen

Article

Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

Insert

CheRIff_EGFP

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pGEMT-PT2A-GFP-Tdtomato-iRFP670

Plasmid

#111812

Purpose

Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A

Depositor

Li Qian

Article

Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

Inserts

GFP
Tdtomato
iRFP670

Use

Cloning intermediate

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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