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20,364 results

Showing: 11941 - 11960 of 20364 results

  5. pDNA2.0 6H-TEV-KRAS G12V

    Plasmid
    #111850
    Purpose
    Expresses activated human KRAS G12V (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.
    Depositor
    Kenneth Westover
    Publication
    Hunter et al Mol Cancer Res. 2015 Sep;13(9):1325-35.
    Insert
    KRAS G12V (KRAS Human)
    Tags
    6xHis-tag and TEV protease cleavage sequence
    Expression
    Bacterial
    Mutation
    Changed Glycine 12 to Valine
    Promoter
    T5
    Available since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    111850-plasmid-map-sequence-id-213464

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  9. AICSDP-52: HIST1H2BJ-mEGFP

    Plasmid
    #109121
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human HIST1H2BJ
    Depositor
    Allen Institute for Cell Science
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HIST1H2BJ Homology Arms with linker-mEGFP (H2BC11 Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available since
    May 4, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    109121-plasmid-map-sequence-id-209767

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  10. pGEX6P1‐hsRNASEH2BCA

    Plasmid
    #108692
    Purpose
    For expression in E coli of N-terminally GST-tagged human RNASEH2B and untagged RNASEH2C and A, allowing affinity purification of the RNase H2 trimeric enzyme
    Depositor
    Andrew Jackson, Martin Reijns
    Publication
    Reijns et al J Biol Chem. 2011 Mar 25;286(12):10530-9
    Inserts
    RNASEH2B (RNASEH2B Human)
    RNASEH2C (RNASEH2C Human)
    RNASEH2A (RNASEH2A Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Shine Dalgarno sequence upstream of start codon a…
    Promoter
    tac
    Available since
    April 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    108692-plasmid-map-sequence-id-208976

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  13. dxCas(3.6)-VPR

    Plasmid
    #108388
    Purpose
    Mammalian expression vector for xCas9 3.6 with VP64-p65-Rta (VPR) fused to its C-terminus
    Depositor
    David Liu
    Publication
    Hu et al Nature. 2018 Feb 28. pii: nature26155. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dxCas(3.6)-VPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    E108G, S217A, A262T, S409I, E480K, E543D, M694I, …
    Available since
    March 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    108388-plasmid-map-sequence-id-207184

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  17. pWS172

    Plasmid
    #90519
    Purpose
    Cas9 gap repair vector - HIS3
    Depositor
    Tom Ellis
    Insert
    Cas9
    Use
    CRISPR
    Expression
    Yeast
    Available since
    Feb. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    90519-plasmid-map-sequence-id-287208

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  19. pRluc-LC3G120A

    Plasmid
    #105003
    Purpose
    Mammalian expression of Rluc-LC3G120A. Can be used for measuring autophagic flux.
    Depositor
    Marja Jããttelã
    Publication
    Farkas et al Methods Enzymol. 2017;588:1-13. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LC3B (Map1lc3b Rat)
    Use
    Luciferase
    Tags
    Rluc with C124A substitution
    Expression
    Mammalian
    Mutation
    Glycine 120 mutated to alanine, prevents lipidati…
    Available since
    Jan. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    105003-plasmid-map-sequence-id-202164

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Showing: 11941 - 11960 of 20364 results