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pGIPZ EF1a IRES miRFP720-P2A-Blast

Plasmid

#248029

Purpose

Backbone that constitutively expresses miRFP720 and a blasticidin resistance gene in human cells

Depositor

Joshua Leonard

Article

Deol et al GEN Biotechnol. 2023 Jun 1;2(3):228-246.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 16, 2026

Availability

Academic Institutions and Nonprofits only

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pSSBIO32_dCas9_RPA0650

Plasmid

#240487

Purpose

CRISPRi-mediated Knockdown of RPA0650

Depositor

Rajib Saha

Article

Kathol et al Appl Environ Microbiol. 2025 Aug 20;91(8

Insert

Cas9 (cas9 Streptococcus pyogenes)

Use

CRISPR

Expression

Bacterial

Mutation

D10A, H840A

Promoter

Plac

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pSSBIO32_dCas9_RPA3717

Plasmid

#240491

Purpose

CRISPRi-mediated Knockdown of RPA3717

Depositor

Rajib Saha

Article

Kathol et al Appl Environ Microbiol. 2025 Aug 20;91(8

Insert

Cas9 (cas9 Streptococcus pyogenes)

Use

CRISPR

Expression

Bacterial

Mutation

D10A, H840A

Promoter

Plac

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pSSBIO32_dCas9_RPA3701

Plasmid

#240490

Purpose

CRISPRi-mediated Knockdown of RPA3701

Depositor

Rajib Saha

Article

Kathol et al Appl Environ Microbiol. 2025 Aug 20;91(8

Insert

Cas9 (cas9 Streptococcus pyogenes)

Use

CRISPR

Expression

Bacterial

Mutation

D10A, H840A

Promoter

Plac

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pSSBIO32_dCas9_RPA1580

Plasmid

#240488

Purpose

CRISPRi-mediated Knockdown of RPA1580

Depositor

Rajib Saha

Article

Kathol et al Appl Environ Microbiol. 2025 Aug 20;91(8

Insert

Cas9 (cas9 Streptococcus pyogenes)

Use

CRISPR

Expression

Bacterial

Mutation

D10A, H840A

Promoter

Plac

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pBEL2782

Plasmid

#232506

Purpose

Expression plasmid of LetB

Depositor

Gira Bhabha, Damian Ekiert

Article

Santarossa et al bioRxiv 2025.03.21.644421

Insert

YebT (letB E. coli)

Tags

QHQ-7xHis

Expression

Bacterial

Mutation

None

Promoter

araBAD

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBEL2800

Plasmid

#232508

Purpose

Expression plasmid of LetB(F468B)

Depositor

Gira Bhabha, Damian Ekiert

Article

Santarossa et al bioRxiv 2025.03.21.644421

Insert

YebT (letB E. coli)

Tags

QHQ-7xHis

Expression

Bacterial

Mutation

LetB F468Amber

Promoter

araBAD

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBEL2472

Plasmid

#232491

Purpose

Genetic complementation plasmid of LetA(Δ2-24) with LetB

Depositor

Gira Bhabha, Damian Ekiert

Article

Santarossa et al bioRxiv 2025.03.21.644421

Insert

YebST (letA, letB E. coli)

Expression

Bacterial

Mutation

LetA Δ2-24

Promoter

T7

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBEL2635

Plasmid

#232499

Purpose

Genetic complementation plasmid of LetA(Δ2-17) with LetB

Depositor

Gira Bhabha, Damian Ekiert

Article

Santarossa et al bioRxiv 2025.03.21.644421

Insert

YebST (letA, letB E. coli)

Expression

Bacterial

Mutation

LetA Δ2-17

Promoter

T7

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBEL2214

Plasmid

#232486

Purpose

Expression plasmid of LetA

Depositor

Gira Bhabha, Damian Ekiert

Article

Santarossa et al bioRxiv 2025.03.21.644421

Insert

YebS (letA E. coli)

Tags

QHQ-7xHis

Expression

Bacterial

Mutation

wild type

Promoter

araBAD

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pmCh-C1 Rab45

Plasmid

#241255

Purpose

For exogenous expression of Rab45 (RASEF) as an N-terminal fusion to RFP in mammalian cells

Depositor

Pietro De Camilli

Article

Hanna et al EMBO J (2025) 44: 6168 - 6195

Insert

Rab45 (RASEF) (RASEF Human)

Tags

RFP

Expression

Mammalian

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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tetO-HyperTRIBE-adar_deaminase-V5-t2a-mCherry

Plasmid

#241314

Purpose

inducible mouse Adar Deaminase domain (HyperTRIBE) for expression in mammalian cells

Depositor

Stavros Lomvardas

Insert

Adar deaminase domain (Adar Mouse)

Tags

V5

Expression

Mammalian

Mutation

E957Q

Promoter

tetO and CMV

Available Since

Oct. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pDonr 201_PHIP

Plasmid

#240328

Purpose

Entry vector for Gateway with PHIP

Depositor

Junjie Chen

Article

Nie et al Genes Dev. 2022 Sep 1;36(17-18):1016-103

Insert

PHIP (PHIP Human)

Expression

Bacterial

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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TEF2pro_mCherry_YFP

Plasmid

#190588

Purpose

Dual reporter backbone plasmid for CCRA experiment- LEU2 Marker

Depositor

Rob Mitra

Article

Recio et al Nucleic Acids Res. 2023 Jun 9;51(10):500

Insert

None

Expression

Yeast

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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AGG1829 OpIE2-Bn

Plasmid

#242438

Purpose

Insect OpIE2 promoter expressing barnase

Depositor

Luke Alphey

Article

Nevard et al Commun Biol. 2025 Aug 4;8(1):1154. doi:

Insert

Barnase (J5X95_RS06160 Bacillus amyloliquefaciens)

Expression

Insect

Promoter

OpIE2

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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AGG1767 PUb-Bs-TRE-Bn

Plasmid

#242441

Purpose

Ae aegypti PUb promoter expressing barstar, and TRE expressing barnase

Depositor

Luke Alphey

Article

Nevard et al Commun Biol. 2025 Aug 4;8(1):1154. doi:

Inserts

Barstar
DsRED
barnase

Expression

Insect

Promoter

AePUb, Hr5/IE1, and TRE

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV2 Bxb1-c26

Plasmid

#242875

Purpose

Mammalian expression of hyperactive Bxb1 Integrase mutant (V122M, A369P, 434G)

Depositor

Jesse Owens

Article

Hew et al Nucleic Acids Res. 2024 Jul 2:gkae534. d

Insert

Bxb1 Integrase

Expression

Mammalian

Mutation

V122M, A369P, 434G

Promoter

CMV

Available Since

Sept. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV2 Bxb1-c15

Plasmid

#242873

Purpose

Mammalian expression of hyperactive Bxb1 Integrase mutant (V122M, E434G)

Depositor

Jesse Owens

Article

Hew et al Nucleic Acids Res. 2024 Jul 2:gkae534. d

Insert

Bxb1 Integrase

Expression

Mammalian

Mutation

V122M, E434G

Promoter

CMV

Available Since

Sept. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV2 Bxb1-c10

Plasmid

#242872

Purpose

Mammalian expression of hyperactive Bxb1 Integrase mutant (I87L, E434G)

Depositor

Jesse Owens

Article

Hew et al Nucleic Acids Res. 2024 Jul 2:gkae534. d

Insert

Bxb1 Integrase

Expression

Mammalian

Mutation

I87L, E434G

Promoter

CMV

Available Since

Sept. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pKG2197_pPV2-ZF10-NS3-p65

Plasmid

#239700

Purpose

BsaI Golden Gate assembly plasmid encoding the SynZiFTR ZF10-NS3-p65 CDS

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

ZF10-NS3-p65

Use

Synthetic Biology

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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