We narrowed to 61,669 results for: promoter
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Plasmid#205412PurposeVector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoterDepositorInsertpU6-_RNA*-pCAG-bpNLS-TadA8e(V106W)-32aa-OgeuIscB*(D61A)-GS-bpNLS-GS-TadA8e(V106W)-bpNLS-bGH polyA-pCMV-mCherry-AmpR
ExpressionMammalianMutationD61A+E85R+H369R+S387R+S457RPromoterhU6, CAG, CMVAvailable sinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
lenti U6-sgRNA-acRNA SYN-dCas9-P2A-EGFP
Plasmid#159086PurposeExpresses dCas9-P2A-EGFP driven by human SYN promoter and empty CRISPR Display sgRNA/accessory RNA from U6 promoter.DepositorInsertempty crRNA-acRNA backbone, dCas9-P2A-EGFP
UseCRISPR and LentiviralTagsEGFP and FLAGExpressionMammalianPromoterhSYN, U6Available sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pgRGFP
Plasmid#82695PurposeExpresses gRNA of interest under U6 promoter (cloning by BpiI) and GFP under PGK promoter.DepositorTypeEmpty backboneUseCRISPRExpressionMammalianPromoterPGK, U6Available sinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CAG-ABEmax-P2A-EGFP-ires-puro
Plasmid#121170PurposeCAG promoter driven expression of ABEmax base editor, EGFP and Puromycin resistance.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertABEmax-P2A-EGFP-ires-puro
UseCRISPRTagsP2A-EGFP-ires-puroExpressionMammalianAvailable sinceJuly 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSBbi G418 Hu Her2
Plasmid#183246PurposeExpression of human HER2 antigen in a Sleeping Beauty transposon vectorDepositorInsertHer2 (ERBB2 Human)
ExpressionMammalianAvailable sinceOct. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSB208
Plasmid#164123PurposePtac driving PP7 repeatsDepositorInsertYFP-PP7
ExpressionBacterialPromoterPtacAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAT5
Plasmid#112586PurposeUsed for expression of protein in E. coli as non-cleavable C-terminal 6His-tag fusionsDepositorTypeEmpty backboneTags6HisExpressionBacterialAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFIN-mIRBP156-CHER-RK-GFP-WPRE
Plasmid#44593DepositorInsertsIRBP156-CHER
RK-GFP
UseLentiviralPromoterhuman rhodopsin kinase (pos 3723-4019) and murine…Available sinceMay 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJK_proK6_eGFP
Plasmid#59850PurposeConstitutively expresses eGFP with proK6 promoterDepositorInsertEnhanced Green Fluorescent Protein
Tags6x-HisExpressionBacterialMutationS2R,S65G,S72APromoterproK6Available sinceOct. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKRG3-mU6-PUb-hSpCas9
Plasmid#162162PurposeExpresses hSpCas9 (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter)DepositorInsertshSpCas9
sgRNA
UseCRISPRTagsN/AExpressionInsectPromoterAe. aegypti PUb and Ae. aegypti U6Available sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMALB
Plasmid#161784PurposeGateway destination vector with bidirectional CMV-PGK promoter for modest expression of gene of interest and TagBFP.DepositorTypeEmpty backboneUseLentiviralExpressionMammalianPromoterBidirectional minhCMV and hPGKAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
p2B-58
Plasmid#177928PurposeExpresses yeGFP under control of doxycycline-responsive TET4 promoter (contains four rtTA binding sites)DepositorPublicationInsertEGFP
ExpressionBacterial and YeastPromoterTET4 (contains four rtTA binding sites)Available sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
dCas9-GCN5 core
Plasmid#179551Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human GCN5 core (aa 473-676) driven by EF-1alpha promoterDepositorInsertS. pyogenes dCas9 with c-terminal fusion of human GCN5 core (aa 473-676) (KAT2A Human)
UseLentiviralTagsFlag TagExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterEF-1alphaAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAM-AAV-hSyn1-EGFP
Plasmid#153205PurposeHuman synapsin-1 (hSyn1) promoter-mediates gene expression in retinal neurons with fluorescent reporterDepositorTypeEmpty backboneUseAAVExpressionMammalianAvailable sinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
isoform PKC Beta (JCE694)
Plasmid#182024Purposeyeast expression of mouse PKC Beta with yeast PKC1 promoter and ADH1 terminatorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPKCBeta (Prkcb Mouse)
ExpressionMammalianPromoteryeast PKC1 promoter (-600 to -1)Available sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
isoform PKC Delta (JCE594)
Plasmid#182025Purposeyeast expression of mouse PKC Delta with yeast PKC1 promoter and ADH1 terminatorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPKC Delta (Prkcd Mouse)
ExpressionMammalianPromoteryeast PKC1 promoter (-600 to -1)Available sinceMay 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-SAGFP
Plasmid#127100PurposeSA-GFP substrate (Addgene #41594) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. SA-GFP cassette contains PGK-puromycin.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSA-GFP reporter
ExpressionMammalianAvailable sinceJune 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
qTAG-AAVS1-Ef1a-Blast
Plasmid#227267PurposeEmpty AAVS1 targeting donor for the insertion of Blast and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.DepositorTypeEmpty backboneUseCRISPR; Donor cassetteExpressionMammalianAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-Bi-gePSI-pCamk2a-AcGFP
Plasmid#133732PurposeExpresses both the gePSI-α-chain and the gePSI-β-chain under control of the inducible bi-directional TRE3G promoter. Expresses constitutively AcGFP under control of a Camk2a promoter.DepositorInsertsgePSI-β-chain
gePSI-α-chain
AcGFP1
UseAAVTagsmurine ornithine decarboxylase - degron (ODC36)ExpressionMammalianPromoterpCamk2a and pTRE3G-BiAvailable sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJH2970
Plasmid#100954PurposeCas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. HIS3MX markerDepositorPublicationTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionYeastAvailable sinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits only