We narrowed to 12,205 results for: 112

1. pLX304-EZH1-D745N

   Plasmid

   #203585

   Purpose

   Expresses V5-tagged mutant version of EZH1 partially resistant to JQ-EZ-05 in mammalian cells.

   Depositor

   Abhishek Chakraborty, William Kaelin

   Article

   Chakraborty et al Sci Transl Med. 2017 Jul 12;9(398):eaal5

   Insert

   EZH1 (EZH1 Human)
   

   Use

   Lentiviral

   Tags

   V5-tagged

   Expression

   Mammalian

   Mutation

   changed Aspartic Acid 745 to Asparagine for parti…

   Promoter

   CMV

   Available Since

   Feb. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-CMV-FLEX-SaCas9-U6-sgKcnma1

   Plasmid

   #209199

   Purpose

   Mutagenesis of Kcnma1

   Depositor

   Larry Zweifel

   Article

   Juarez et al Sci Adv. 2023 Aug 11;9(32):eadg8869. doi

   Insert

   Kcnma1 (Kcnma1 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Promoter

   CMV

   Available Since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLKO shLuc/FLAG Metap2

   Plasmid

   #209316

   Purpose

   Lentiviral construct expressing FLAG Metap2 and shRNA against Luciferase (control shRNA)

   Depositor

   Roberto Zoncu

   Article

   Shin et al Science. 2022 Sep 16;377(6612):1290-1298

   Insert

   METAP2 (METAP2 human, Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Dec. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLKO shLYCHOS/LYCHOS WT-FLAG

   Plasmid

   #209315

   Purpose

   Lentiviral construct expressing shRNA against LYCHOS and LYCHOS WT-FLAG (resistant to shRNA)

   Depositor

   Roberto Zoncu

   Article

   Shin et al Science. 2022 Sep 16;377(6612):1290-1298

   Insert

   LYCHOS (GPR155 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Dec. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLKO shLYCHOS/FLAG Metap2

   Plasmid

   #209314

   Purpose

   Lentiviral construct expressing FLAG Metap2 and shRNA against LYCHOS

   Depositor

   Roberto Zoncu

   Article

   Shin et al Science. 2022 Sep 16;377(6612):1290-1298

   Insert

   LYCHOS (GPR155 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Dec. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pOTTC1822 - pAAV SYN1 DIO hChR2(H134R)-V5-ER-LBD (ChRERa)

   Plasmid

   #201821

   Purpose

   An adeno-associated viral vector expressing Cre-dependent channelrhodopsin-2 fused to a V5 epitope and the ligand-binding domain of estrogen receptor (ChRERa) from a synapsin promoter, for use in PET

   Depositor

   Christopher Richie

   Article

   Bonaventura et al Sci Transl Med. 2023 Jul 26;15(706):eadd

   Insert

   hChR2(H134R)-V5-ER-LBD (ChRERa)
   

   Use

   AAV

   Promoter

   SYN1

   Available Since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pOTTC1845 - pAAV CaMKII hChR2(H134R)-V5-ER-LBD (ChRERa)

   Plasmid

   #201822

   Purpose

   An adeno-associated viral vector expressing channelrhodopsin-2 fused to a V5 epitope and the ligand-binding domain of estrogen receptor (ChRERa) from a CaMKii promoter, for use in PET imaging

   Depositor

   Christopher Richie

   Article

   Bonaventura et al Sci Transl Med. 2023 Jul 26;15(706):eadd

   Insert

   hChR2(H134R)-V5-ER-LBD (ChRERa)
   

   Use

   AAV

   Promoter

   CaMKii

   Available Since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHAGE2-TetO-hSOX2-C17

   Plasmid

   #206385

   Purpose

   Expresses human SOX2-C17 in mammalian cells, for lentivirus generation.

   Depositor

   Ralf Jauch

   Article

   Weng et al Sci Adv. 2023 Aug 25;9(34):eadh2501. doi

   Insert

   SOX2-C17 chimera (SOX2, SOX17 Human)
   

   Use

   Lentiviral

   Available Since

   Oct. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pQCXIZ GFP PODXL delta PBM

   Plasmid

   #202415

   Purpose

   Expression of GFP-tagged PODXL PDZ-binding motif mutation (PBM; doesn't bind NHERF1/2)

   Depositor

   David Bryant

   Article

   Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

   Insert

   PODXL (PODXL Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Mutation

   Lacking DTHL

   Available Since

   Aug. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pQCXIZ GFP PODXL FBM

    Plasmid

    #202417

    Purpose

    Expression of GFP-tagged PODXL FERM-binding motif mtuation (FBM; doesn't bind Ezrin)

    Depositor

    David Bryant

    Article

    Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

    Insert

    PODXL (PODXL Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Mutation

    H477/R479/S481>AAA mutation

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFB-CG-LYCHOS FP>IA-HA-GFP

    Plasmid

    #199688

    Purpose

    Expression of LYCHOS FP>IA mutant in insect cells

    Depositor

    Roberto Zoncu

    Article

    Shin et al Science. 2022 Sep 16;377(6612):1290-1298

    Insert

    LYCHOS (GPR155 Human)
    

    Tags

    10xHis, 6xHis, EGFP, HA, and HRV 3C

    Expression

    Insect

    Mutation

    F43 and P44 mutated to I and A respectively

    Available Since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28a LZ LED 4CA-FLAG

    Plasmid

    #199681

    Purpose

    Bacterial expression of Leucine Zipper (LZ) LYCHOS LED 4CA-Flag

    Depositor

    Roberto Zoncu

    Article

    Shin et al Science. 2022 Sep 16;377(6612):1290-1298

    Insert

    Leucine zipper LYCHOS LED 4CA (GPR155 Human)
    

    Tags

    6xHis and Flag

    Expression

    Bacterial

    Mutation

    C595.604.629.638 of LYCHOS mutated to A

    Promoter

    T7 promoter

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28a LZ LED Y551A-FLAG

    Plasmid

    #199680

    Purpose

    Bacterial expression of Leucine Zipper (LZ) LYCHOS LED Y551A-Flag

    Depositor

    Roberto Zoncu

    Article

    Shin et al Science. 2022 Sep 16;377(6612):1290-1298

    Insert

    Leucine zipper LYCHOS LED Y551A (GPR155 Human)
    

    Tags

    6xHis and Flag

    Expression

    Bacterial

    Mutation

    Y551 of LYCHOS mutated to A

    Promoter

    T7 promoter

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRK5-Myc-PSK2 (1-416) (K57A)

    Plasmid

    #197124

    Purpose

    Expression of kinase-deficient Myc-tagged PSK2 (aa1-416, K57A) / TAOK1 (aa1-416, K57A) in mammalian cells

    Depositor

    Jonathan Morris

    Article

    Tavares et al J Biol Chem. 2013 May 24;288(21):15418-2

    Insert

    TAOK1 (aa1-416, K57A) (TAOK1 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Changed K 57 to A and deleted C-terminus

    Promoter

    SV40

    Available Since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pD649-HAsp-LRRN3-COMP5AP-AviTag-9xHis

    Plasmid

    #157491

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    LRRN3 (LRRN3 Human)
    

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pB80-hsKIF5B(1-560)Rigor-L-mVenus-L-SspB(micro)

    Plasmid

    #193721

    Purpose

    Optogenetic coupling to dimeric human kinesin-1 (KIF5B aa1-807) via SSPB(micro) for anterograde transport

    Depositor

    Lukas Kapitein

    Article

    Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

    Insert

    hsKIF5B(1-560)Rigor-L-mVenus-L-SspB(micro) (KIF5B Synthetic, Human)
    

    Tags

    mVenus-SSPB(micro)

    Expression

    Mammalian

    Mutation

    hsKIF5B(aa1-560), RIGOR: G234A; mVenus: Met1Del, …

    Promoter

    Chicken beta-actin

    Available Since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MDM4_Deletion_Upstream_gRNA_2

    Plasmid

    #195135

    Purpose

    gRNA in a third generation Cas9 vector with GFP, targeting region immediately upstream of MDM4, to be used with MDM4_Deletion_Downstream_gRNA_1/2 for MDM4 deletion

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    MDM4 Deletion Upstream gRNA 2 (MDM4 Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MDM4_Deletion_Upstream_gRNA_1

    Plasmid

    #195134

    Purpose

    gRNA in a third generation Cas9 vector with GFP, targeting region immediately upstream of MDM4, to be used with MDM4_Deletion_Downstream_gRNA_1/2 for MDM4 deletion

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    MDM4 Deletion Upstream gRNA 1 (MDM4 Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pαH-S-GSAS-B.1.351.v4

    Plasmid

    #173788

    Purpose

    Mammalian expression of SARS-CoV-2 Spike protein South African variant version 4

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Science. 2021 Jun 24. pii: science.abi62

    Insert

    Spike (S-GSAS-B.1.351 variant version 4) (S Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Available Since

    Jan. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTW5126

    Plasmid

    #129732

    Purpose

    polyhistidine replacement of EatA passenger DUF corresponding to amino acids E541 -Q617 of the native EatA protein

    Depositor

    James Fleckenstein

    Article

    Sheikh et al Infect Immun. 2021 Nov 22:IAI0057221. do

    Insert

    eatA-DUF::His8
    

    Tags

    9His tag

    Expression

    Bacterial

    Mutation

    replaces the domain of unknown function correspon…

    Promoter

    araBAD

    Available Since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge