1,878 results

1. pMDJ10 - pEXP[Peft-3 | tagBFP2 (NLS) | tbb-2 UTR]

   Plasmid

   #159798

   Purpose

   Expression construct with ubiquitous expression to test fluorescence level on microscope

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   pEXP[Peft-3 | tagBFP2 (NLS) | tbb-2 UTR]
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCFJ1532 - pEXP[Psmu-1 | mosase (PATCs) | smu-1 UTR]

   Plasmid

   #159807

   Purpose

   Optimized enzyme for genome editing in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   pEXP[Psmu-1 | mosase (PATCs) | smu-1 UTR]
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMLS597

   Plasmid

   #188884

   Purpose

   dpy-10 sgRNA expression vector

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

   Insert

   PU6(K09B11.12)::dpy-10 sgRNA (dpy-10 Nematode)
   

   Expression

   Worm

   Available Since

   Aug. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDD287 (mKate2^SEC^3xMyc

   Plasmid

   #70685

   Purpose

   mKate2^SEC^3xMyc vector with ccdB sites for cloning homology arms

   Depositor

   Bob Goldstein

   Article

   Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

   Insert

   mKate2-C1^SEC^3xFlag
   

   Use

   CRISPR and Cre/Lox

   Tags

   3xMyc and C. elegans codon-optimized mKate2

   Expression

   Worm

   Available Since

   Jan. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSEM233 - [Pmlc-1 | tagRFP-T | cbr-tbb-2 UTR]

   Plasmid

   #159899

   Purpose

   Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

   Insert

   Pmlc-1 | tagRFP | cbr-tbb-2 UTR
   

   Expression

   Worm

   Available Since

   Oct. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJW2098

   Plasmid

   #163094

   Purpose

   CRISPR repair template: add insertion site specific homology arms by PCR before insertion

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   30aa linker::mScarlet (dpi)::AID*::3xFLAG::10aa linker
   

   Use

   CRISPR

   Expression

   Worm

   Mutation

   Silent mutations to remove piRNA sites

   Available Since

   Jan. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. L4440_BioBrick-hif-1-sgRNA-B

   Plasmid

   #177785

   Purpose

   Overexpression of sgRNAs in E. coli HT115 (targeting C. elegans hif-1 promoter)

   Depositor

   Michael Ristow

   Article

   Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

   Insert

   hif-1 (hif-1 Nematode)
   

   Expression

   Bacterial

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJRK248

   Plasmid

   #173755

   Purpose

   co-injection plasmid

   Depositor

   Mike Boxem

   Article

   Kroll et al Genetics. 2021 Dec 10;219(4). pii: 63804

   Insert

   Prps-0::HygR::unc-54 3'UTR; Psqt-1::sqt-1::sqt-1 3' UTR
   

   Expression

   Worm

   Available Since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCFJ350 - MCS(ttTi5605, II)

   Plasmid

   #34866

   Depositor

   Erik Jorgensen

   Article

   Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

   Insert

   ttTi5605 targeting
   

   Use

   Worm targeting

   Available Since

   Feb. 1, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSEZ-1

    Plasmid

    #51868

    Purpose

    Expression of cpYFP with a mitochondrial-targeting sequence (mt-cpYFP) in C. elegans

    Depositor

    Meng-Qiu Dong

    Article

    Shen et al Nature. 2014 Feb 12. doi: 10.1038/nature

    Insert

    psdhb-1::mtLS-cpYFP
    

    Expression

    Worm

    Promoter

    sdhb-1

    Available Since

    March 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET_RGG-GFP-RGG

    Plasmid

    #124948

    Purpose

    Expression of RGG-GFP-RGG

    Depositor

    Matthew Good, Benjamin Schuster, Daniel Hammer

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
    

    Tags

    EGFP and His

    Expression

    Bacterial

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSEM246 - MCS mosTI unc-119 target

    Plasmid

    #159821

    Purpose

    Single copy or array insertion by MosTI using split unc-119 selection

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    MCS mosTI unc-119 target
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPK605

    Plasmid

    #38148

    Depositor

    Patricia Kuwabara

    Inserts

    unc-119 rescuing fragment (unc-119 promoter, unc-119 and unc-119 3'UTR) (unc-119 Nematode)
    pie-1 promoter - unique MluI/BamHI cloning site - pie-1 3'UTR
    

    Expression

    Worm

    Promoter

    pie-1 promoter and unc-119

    Available Since

    Oct. 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSF11-wmTagBFP2

    Plasmid

    #184934

    Purpose

    Codon optimized blue fluorescent protein mTagBFP2 in C.elegans expresison plasmid

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    mTagBFP2
    

    Expression

    Worm

    Promoter

    tag-168

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJW1310

    Plasmid

    #61253

    Purpose

    Template for cloning U6 promoter to generate new PU6::sgRNAs by PCR-fusion

    Depositor

    Jordan Ward

    Article

    Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

    Insert

    U6 promoter
    

    Use

    CRISPR; Cloning template

    Available Since

    Dec. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPICZA_CeTRIC-B1_Del

    Plasmid

    #87344

    Purpose

    Inducible expression of CeTRIC-B1 in P.Pichia strains

    Depositor

    Zhenfeng Liu

    Article

    Yang et al Nature. 2016 Oct 27;538(7626):537-541. d

    Insert

    TRIC-B1
    

    Tags

    polyhistidine

    Expression

    Yeast

    Mutation

    Δ248-297

    Promoter

    AOX1

    Available Since

    Feb. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCI_AFF-1-FLAG_H2B-RFP

    Plasmid

    #240506

    Purpose

    Cell fusion assays

    Depositor

    Benjamin Podbilewicz

    Article

    Moi et al Nat Commun. 2022 Jul 6;13(1):3880. doi:

    Insert

    AFF-1
    

    Tags

    H2B-RFP

    Expression

    Mammalian

    Available Since

    July 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDD286 (TagRFP^SEC^3xMyc)

    Plasmid

    #70684

    Purpose

    TagRFP-T^SEC^3xMyc vector with ccdB sites for cloning homology arms.

    Depositor

    Bob Goldstein

    Insert

    TagRFP-T-C1^SEC^3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xMyc and C. elegans codon-optimized TagRFP-T

    Expression

    Worm

    Available Since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAD43-daf-16 RNAi

    Plasmid

    #34833

    Depositor

    Cynthia Kenyon

    Article

    Dillin et al Science. 2002 Oct 25;298(5594):830-4.

    Insert

    daf-16 (daf-16 Nematode)
    

    Use

    RNAi

    Expression

    Bacterial

    Available Since

    June 13, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pJRK261

    Plasmid

    #173754

    Purpose

    CeLINC plasmid

    Depositor

    Mike Boxem

    Article

    Kroll et al Genetics. 2021 Dec 10;219(4). pii: 63804

    Insert

    (empty)::CRY2(olig)::VHH(GFP)::T2A::CIBN-3xFLAG::MP::unc-54 3' UTR
    

    Expression

    Worm

    Available Since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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