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2,058 results

Showing: 101 - 120 of 2058 results

  2. pLT 653

    Plasmid
    #59996
    Purpose
    Expresses a hybrid dsRNA corresponding to the Caenorhabditis elegans dhc-3/klp-16 genes in bacteria; ingestion of the bacteria by C elegans produces an RNAi response & leads to more male progeny
    Depositor
    Lisa Timmons
    Publication
    Timmons et al FEBS Lett. 2014 Jul 24. pii: S0014-5793(
    Inserts
    dhc-3 (dhc-3 Nematode)
    klp-16 (klp-16 Nematode)
    Expression
    Bacterial
    Mutation
    partial genomic
    Available since
    April 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    59996-plasmid-map-sequence-id-110769

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  6. SP6-hCas9-Ce-mRNA

    Plasmid
    #47911
    Purpose
    In vitro transcription of a mRNA encoding humanized Cas9 nuclease, with 3' UTRs suitable for germline expression in C. elegans, for doing CRISPR-Cas by RNA injection. Uses SP6 RNA polymerase.
    Depositor
    Paul Sternberg
    Publication
    Chiu et al Genetics. 2013 Aug 26.
    Insert
    hCas9 with C. elegans 5' and 3' UTRs
    Use
    CRISPR
    Promoter
    SP6
    Available since
    Sept. 13, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    47911-plasmid-map-sequence-id-68528

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  9. pJW2171

    Plasmid
    #163095
    Purpose
    CRISPR repair template: add insertion site specific homology arms by PCR before insertion
    Depositor
    Jordan Ward
    Publication
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    30aa linker::mNeonGreen (dpi)::AID*::3xFLAG::10aa linker
    Use
    CRISPR
    Expression
    Worm
    Mutation
    Silent mutations to remove piRNA sites
    Available since
    Jan. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    163095-plasmid-map-sequence-id-320590

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  11. pCFJ150-mCherry(dpiRNA)::ANI-1(AHPH)

    Plasmid
    #107939
    Purpose
    pie-1p::mCherry(dpiRNA)::ANI-1(AHPH)::pie-1 3'UTR construct used for MosSCI in nematode. mCherry is optimized by removing all piRNA targeting sites to allow germline expression. RhoA biosenser.
    Depositor
    Heng-Chi Lee
    Publication
    Zhang et al Science. 2018 Feb 2;359(6375):587-592. d
    Insert
    mCherry(dpiRNA)::ANI-1(AHPH)
    Expression
    Worm
    Promoter
    pie-1
    Available since
    April 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    107939-plasmid-map-sequence-id-207466

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  17. pLT 651

    Plasmid
    #59998
    Purpose
    Expresses a hybrid dsRNA corresponding to the Caenorhabditis elegans klp-16/him-8 genes in bacteria; ingestion of the bacteria by C elegans produces an RNAi response & leads to more male progeny
    Depositor
    Lisa Timmons
    Publication
    Timmons et al FEBS Lett. 2014 Jul 24. pii: S0014-5793(
    Inserts
    klp-16 (klp-16 Nematode)
    him-8 (him-8 Nematode)
    Expression
    Bacterial
    Mutation
    partial genomic
    Available since
    April 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    59998-plasmid-map-sequence-id-110771

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  20. pcDNA3.1 V5 His-TOPO-GluCl optalpha-mYFP L9'F (Opt α-GluClv2.0)

    Plasmid
    #47387
    Purpose
    allow pharmacologically induced silencing of electrical activity in CNS neurons upon exposure to the anthelmintic drug ivermectin
    Depositor
    Henry Lester
    Publication
    Frazier et al J Biol Chem. 2013 May 29.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    glutamate-gated chloride Channels
    Expression
    Mammalian
    Mutation
    Leu9 prime mutated to F (Phenylalanine in M3-M4 l…
    Promoter
    CMV
    Available since
    Aug. 28, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    47387-plasmid-map-sequence-id-67454

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Showing: 101 - 120 of 2058 results