We narrowed to 1,005 results for: Psp

1. pSplit-OC-U5-40K

   Plasmid

   #51741

   Purpose

   Express U5-40K-LucC fusion protein in mammalian cells

   Depositor

   Hiroyoshi Ariga

   Article

   Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

   Insert

   U5-40K (SNRNP40 Human)
   

   Use

   Tags

   FLAG and Luciferase (394-550aa)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 22, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pSP72 GSK3beta

   Plasmid

   #60125

   Purpose

   for transcription of GSK3beta

   Depositor

   Andrew McMahon

   Insert

   Glycogen synthase kinase 3 beta (Gsk3b Mouse)
   

   Use

   Cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSpyTag-GlpG

   Plasmid

   #79054

   Purpose

   Expresses SpyTagged GlpG protein

   Depositor

   James Bowie

   Article

   Min et al Protein Sci. 2016 Aug;25(8):1535-44. doi

   Insert

   SpyTag-GlpG (glpG )
   

   Use

   Tags

   6xHis and SpyTag

   Expression

   Bacterial

   Mutation

   GlpG protein employed here corresponds to residue…

   Promoter

   Trc

   Available since

   June 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-PSPC1-CT

   Plasmid

   #124374

   Purpose

   expresses EGFP-PSPC1-CT131 in mammalian cell

   Depositor

   Yuh-Shan Jou

   Article

   Lang et al Nat Commun. 2019 Dec 16;10(1):5716. doi:

   Insert

   PSPC1 CT (PSPC1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. 2X_pX458_pSpCas9(BB)-2A-GFP_NANOG_bKO

   Plasmid

   #172224

   Purpose

   Cas9-2A-GFP expression vector bearing two sgRNAs targeting the telomeric human NANOG CTCF-boundary.

   Depositor

   Alexander Meissner

   Article

   Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

   Insert

   sgRNA
   

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPSP6+t-gfp

   Plasmid

   #48130

   Purpose

   Shuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-SP6-inducible promoter

   Depositor

   Dieter Jahn

   Article

   Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

   Insert

   gfp
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 21, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSP64T-dnWnt-11

   Plasmid

   #16857

   Depositor

   Jim Smith

   Article

   Tada et al Development. 2000 May . 127(10):2227-38.

   Insert

   dnWnt-11 (wnt11b.L Frog)
   

   Use

   Xenopus expression

   Tags

   Expression

   Mutation

   amino acids 1-282. Dominant Negative.

   Promoter

   Available since

   May 27, 2008

   Availability

   Academic Institutions and Nonprofits only

8. pSP64T acvr1ba*

   Plasmid

   #185409

   Purpose

   Constitutively active form of acvr1ba (Nodal receptor). For in vitro transcription (SP6)

   Depositor

   Nicolas David

   Article

   Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

   Insert

   acvr1ba (acvr1ba Zebrafish)
   

   Use

   In vitro synthesis of mrna

   Tags

   Expression

   Mammalian

   Mutation

   changed Threonine 206 to Aspartic acid

   Promoter

   Available since

   June 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. BII-CPspaH2B

   Plasmid

   #133385

   Purpose

   Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)

   Depositor

   Blair Madison

   Article

   Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. MEIS1A-pSP65

    Plasmid

    #21014

    Depositor

    Corey Largman

    Article

    Shen et al Mol Cell Biol. 1997 Nov;17(11):6448-58

    Insert

    MEIS1A (Meis1 Mouse)
    

    Use

    In vitro tnt

    Tags

    Flag

    Expression

    Mutation

    Promoter

    Available since

    Aug. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

11. pSPL3-ABCC8-27

    Plasmid

    #135919

    Purpose

    Encodes ABCC8 exon 27 wild type for the analysis of splicing variants

    Depositor

    Diana Valverde Pérez

    Article

    Lago-Docampo et al Sci Rep. 2020 Sep 15;10(1):15135. doi: 1

    Insert

    ATP-binding Cassette subfamily C member 8 (ABCC8 Human)
    

    Use

    Exon trapping

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. MEIS1B-pSP65

    Plasmid

    #21019

    Depositor

    Corey Largman

    Article

    Shen et al Mol Cell Biol. 1997 Nov;17(11):6448-58

    Insert

    MEIS1B (Meis1 Mouse)
    

    Use

    In vitro tnt

    Tags

    Flag

    Expression

    Mutation

    Promoter

    Available since

    Aug. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

13. pSP64R1 SHP2 WT

    Plasmid

    #8325

    Depositor

    Ben Neel

    Article

    O'Reilly et al Mol Cell Biol 2000 Jan;20(1):299-311.

    Insert

    SHP2 (PTPN11 Human)
    

    Use

    Xenopus expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 17, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSPDEF-donor

    Plasmid

    #113828

    Purpose

    CRISPR donor plasmid to tag human transcription factor SPDEF with GFP

    Depositor

    Kevin White

    Insert

    SPDEF homology arms flanking EGFP-IRES-Neo cassette (SPDEF Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. PBX2-pSP64

    Plasmid

    #21032

    Depositor

    Corey Largman

    Insert

    PBX2 (PBX2 Human)
    

    Use

    In vitro tnt

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

16. pEC2796 (pSpy0C4)

    Plasmid

    #220280

    Purpose

    integrative plasmid for heterologous gene expression in S. pyogenes SF370, integrates into the sagB locus via homologous recombination

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pscALPSpuro-MDA5delta2CARD

    Plasmid

    #174225

    Purpose

    Expresses human MDA5 that lacks 2 CARD domains

    Depositor

    Jeremy Luban

    Article

    Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

    Insert

    MDA5 (IFIH1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    deletion of 2CARD

    Promoter

    SFFV

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSpyA-GFP

    Plasmid

    #220189

    Purpose

    sgRNA expression vector - pEM7 promoter with GFP in sgRNA site

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    pEM7 promoter with GFP in sgRNA site
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    monomeric superfolder green fluorescent protein

    Promoter

    pEM7

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSpyB-GFP

    Plasmid

    #220190

    Purpose

    sgRNA expression vector - pBG35 promoter with GFP in sgRNA site

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    pBG35 promoter with GFP in sgRNA site
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    monomeric superfolder green fluorescent protein

    Promoter

    pBG35

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSpyD-GFP

    Plasmid

    #220192

    Purpose

    sgRNA expression vector - pBG28 promoter with GFP in sgRNA site

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    pBG28 promoter with GFP in sgRNA site
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    monomeric superfolder green fluorescent protein

    Promoter

    pBG28

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. pSPgRNA_-60_Enh_2

    Plasmid

    #210622

    Purpose

    gRNA target sequences for TAL1 -60 Enhancer

    Depositor

    Maayan Salton

    Article

    Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

    Insert

    TAL1 -60 Enhancer gRNA (TAL1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSPgRNA_-60_Enh_1

    Plasmid

    #210621

    Purpose

    gRNA target sequences for TAL1 -60 Enhancer

    Depositor

    Maayan Salton

    Article

    Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

    Insert

    TAL1 -60 Enhancer gRNA (TAL1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSPgRNA_-60_Enh_4

    Plasmid

    #210624

    Purpose

    gRNA target sequences for TAL1 -60 Enhancer

    Depositor

    Maayan Salton

    Article

    Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

    Insert

    TAL1 -60 Enhancer gRNA (TAL1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSPgRNA_-60_Enh_3

    Plasmid

    #210623

    Purpose

    gRNA target sequences for TAL1 -60 Enhancer

    Depositor

    Maayan Salton

    Article

    Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

    Insert

    TAL1 -60 Enhancer gRNA (TAL1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. 2X_pX458_pSpCas9(BB)-2A-GFP_eSOX17.2_KO

    Plasmid

    #195495

    Purpose

    Cas9-2A-GFP expression vector bearing two sgRNAs targeting the core endoderm distal enhancer of human SOX17

    Depositor

    Alexander Meissner

    Article

    Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

    Insert

    sgRNA (SOX17 Human)
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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26. pSP64-mCherry

    Plasmid

    #135597

    Purpose

    Expression of mCherry mRNA for microinjection into S. purpuratus

    Depositor

    Mamiko Yajima

    Article

    Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

    Insert

    mCherry
    

    Use

    In vitro transcription (mrna synthesis)

    Tags

    Expression

    Mutation

    Promoter

    Unknown

    Available since

    Jan. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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27. pSpCas9(BB)-2A-Blast_HP1beta_gRNA

    Plasmid

    #127908

    Purpose

    WT Cas9 Vector with Blasticidin Selection Marker targeting the 5' end of the human HP1beta gene

    Depositor

    Mark Groudine

    Insert

    gRNA for Human 5' HP1beta
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSP64T-BMP4

    Plasmid

    #117254

    Purpose

    mRNA synthesis

    Depositor

    Gerald Thomsen

    Article

    Nishimatsu et al Mech Dev. 1998 Jun;74(1-2):75-88.

    Insert

    BMP4
    

    Use

    Xenopus expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. XActB-pSP64T

    Plasmid

    #32994

    Depositor

    Sergei Sokol

    Article

    Sokol et al Cell. 1991 Nov 15;67(4):741-52.

    Insert

    activin B
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 5, 2012

    Availability

    Academic Institutions and Nonprofits only

30. pSpCas9_T2A_mCherry sg1-sg2

    Plasmid

    #192479

    Purpose

    All-in-one plasmid containing both guides for cutting BFP region in DSB Spectrum V1

    Depositor

    Michael Yaffe

    Article

    van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

    Insert

    SpCas9
    

    Use

    Tags

    T2A mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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