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We narrowed to 548 results for: Nras

Showing: 201 - 240 of 548 results

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bdSUMO-5.3-GFP-HiBit

Plasmid

#171014

Purpose

For bacterial expression of bdSUMO tagged muGFP with 5.3 peptide and NanoLuc complementation peptide variant #86

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

14x-His-bdSUMO-5.3-muGFP-HiBiT

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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Opto-GPR148

Plasmid

#106048

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR148; Opto-GPR148; E2)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR148 (GPR148 Human, Bovine)

Use

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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Opto-GPR78

Plasmid

#106033

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR78; Opto-GPR78; C11)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR78 (GPR78 Human, Bovine)

Use

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Opto-GPR42

Plasmid

#106022

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR42; Opto-GPR42; B12)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR42 (GPR42 Human, Bovine)

Use

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Opto-GPR32

Plasmid

#106015

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR32; Opto-GPR32; B5)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR32 (GPR32 Human, Bovine)

Use

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
IFN-Beta_pGL3

Plasmid

#102597

Purpose

IFN-beta promoter driving luciferase. Use in IFN reporter assay.

Depositor

Nicolas Manel

Article

Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

Insert

Human IFN-Beta promoter (IFNB1 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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6His-MBP-TEV-huAsCpf1

Plasmid

#90095

Purpose

Bacterial expression plasmid for protein purification

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Insert

huAsCpf1

Use

Tags

3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…

Expression

Bacterial

Mutation

Promoter

Available since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDH-EF1-Large BiT - SNAP-tag - Beta-actin

Plasmid

#171019

Purpose

Lentiviral expression of a Large BiT-SNAP-tag-Beta-actin fusion in mammalian cells

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

LargeBit-SNAPtag- Beta-actin

Use

Lentiviral and Luciferase

Tags

Beta actin and LargeBiT

Expression

Mammalian

Mutation

Promoter

EF1

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-mCherry-Cre-blast

Plasmid

#179390

Purpose

Lentiviral expression of Cre recombinase and mCherry reporter from CMV promoter (separated by P2A peptide), and blasticidin resistance gene for selection in mammalian cells.

Depositor

Moshe Szyf

Article

Sapozhnikov et al Nat Commun. 2021 Sep 29;12(1):5711. doi:

Insert

mCherry (P2A) Cre recombinase

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Hygro(+)-mscGAS

Plasmid

#102607

Purpose

Mammalian expression of mouse cGAS

Depositor

Nicolas Manel

Article

Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

Insert

cGAS (Cgas Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCRISPRv2 sgIRF3#5

Plasmid

#127642

Purpose

Knock-out of human IRF3

Depositor

Nicolas Manel

Article

Gratia et al J Exp Med. 2019 May 6;216(5):1199-1213.

Insert

IRF3 sgRNA (IRF3 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
6His-MBP-TEV-huLbCpf1

Plasmid

#90096

Purpose

Bacterial expression plasmid for protein purification

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Insert

huLbCpf1

Use

Tags

3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…

Expression

Bacterial

Mutation

Promoter

Available since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pY109 (lenti-LbCpf1)

Plasmid

#84740

Purpose

Lenti virus delivery of LbCpf1 and crRNA guide

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Inserts

huLbCpf1
puromycin resistance gene

Use

Lentiviral

Tags

3xHA, NLS, and P2A

Expression

Mutation

Promoter

EFS

Available since

Nov. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pTRIP-CMV-Puro-2A-cGAS

Plasmid

#102612

Purpose

Lentivector expressing cGAS

Depositor

Nicolas Manel

Article

Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

Insert

cGAS (CGAS Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Hygro(+)-PSTCD-cGAS

Plasmid

#102605

Purpose

Mammalian expression of cGAS fused to PSTCD tag

Depositor

Nicolas Manel

Article

Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

Insert

cGAS (CGAS Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pNAD1

Plasmid

#154347

Purpose

pUC19 backbone with editing template for homologous recombination mediated deletion of Nitrate reductase gene of N. oceanica and selection based on zeocin resistance

Depositor

John van der Oost

Article

Naduthodi et al Biotechnol Biofuels. 2019 Mar 25;12:66.

Insert

ShBle

Use

Microalgae

Tags

Expression

Bacterial

Mutation

none

Promoter

Violaxanthin Chlorophyll a Binding Protein Precur…

Available since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCRISPRv2 Neo sgTREX1

Plasmid

#127645

Purpose

Knock-out of human TREX1 with NeoR

Depositor

Nicolas Manel

Article

Gratia et al J Exp Med. 2019 May 6;216(5):1199-1213.

Insert

TREX1 sgRNA (TREX1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1-puro-irf3-shrna1

Plasmid

#127648

Purpose

Knock-down of human IRF3

Depositor

Nicolas Manel

Article

Gratia et al J Exp Med. 2019 May 6;216(5):1199-1213.

Insert

IRF3 shRNA (IRF3 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Opto-GPR55

Plasmid

#106026

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR55; Opto-GPR55; C4)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR55 (GPR55 Human, Bovine)

Use

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCRISPRv2 sgBLM10

Plasmid

#127643

Purpose

Knock-out of human BLM

Depositor

Nicolas Manel

Article

Gratia et al J Exp Med. 2019 May 6;216(5):1199-1213.

Insert

IRF3 sgRNA (IRF3 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Opto-GPR4

Plasmid

#106001

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR4; Opto-GPR4; A3)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR4 (GPR4 Human, Bovine)

Use

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1-puro-irf3-shrna5

Plasmid

#127649

Purpose

Knock-down of human IRF3

Depositor

Nicolas Manel

Article

Gratia et al J Exp Med. 2019 May 6;216(5):1199-1213.

Insert

IRF3 shRNA (IRF3 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Opto-GPR3

Plasmid

#106000

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR3; Opto-GPR3; A2)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR3 (GPR3 Human, Bovine)

Use

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTarget-RFP-GFP operon

Plasmid

#192279

Purpose

RFP GFP fusion protein expressed under a constitutive promoter

Depositor

John van der Oost

Article

Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

Insert

RFP GFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

PlacIq

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMB41

Plasmid

#73087

Purpose

N-terminal Avi-2xTEV-GFP vector, for fosmid recombineering

Depositor

Mike Boxem

Article

Waaijers et al BMC Biol. 2016 Aug 9;14:66. doi: 10.1186

Insert

Avi-GFP tag

Use

Tags

Avi and GFP

Expression

Worm

Mutation

Promoter

Available since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMB72

Plasmid

#73088

Purpose

C-terminal GFP-2xTEV-Avi vector, for fosmid recombineering

Depositor

Mike Boxem

Article

Waaijers et al BMC Biol. 2016 Aug 9;14:66. doi: 10.1186

Insert

GFP-Avi tag

Use

Tags

GFP and Avi

Expression

Worm

Mutation

Promoter

Available since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMB71

Plasmid

#73085

Purpose

Expresses C. elegans optimized BirA under control of the intestinal elt-2 promoter

Depositor

Mike Boxem

Article

Waaijers et al BMC Biol. 2016 Aug 9;14:66. doi: 10.1186

Insert

BirA

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMB73

Plasmid

#73086

Purpose

Expresses C. elegans optimized BirA under control of the seam and hypodermis wrt-2 promoter

Depositor

Mike Boxem

Article

Waaijers et al BMC Biol. 2016 Aug 9;14:66. doi: 10.1186

Insert

BirA

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Opto-GPR27

Plasmid

#106013

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR27; Opto-GPR27; B3)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR27 (GPR27 Human, Bovine)

Use

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMB37

Plasmid

#73084

Purpose

Expresses C. elegans optimized BirA under control of the ubiquitous rps-27 promoter

Depositor

Mike Boxem

Article

Waaijers et al BMC Biol. 2016 Aug 9;14:66. doi: 10.1186

Insert

BirA

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Opto-GPR150

Plasmid

#106050

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR150; Opto-GPR150; E4)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR150 (GPR150 Human, Bovine)

Use

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Opto-GPR173

Plasmid

#106058

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR173; Opto-GPR173; E12)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR173 (GPR173 Human, Bovine)

Use

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Opto-GPR31

Plasmid

#106014

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR31; Opto-GPR31; B4)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR31 (GPR31 Human, Bovine)

Use

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Opto-GPR25

Plasmid

#106011

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR25; Opto-GPR25; B1)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR25 (GPR25 Human, Bovine)

Use

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Opto-GPR21

Plasmid

#106009

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR21; Opto-GPR21; A11)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR21 (GPR21 Human, Bovine)

Use

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Opto-GPR1

Plasmid

#105999

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR1; Opto-GPR1; A1)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR1 (GPR1 Human, Bovine)

Use

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBSII-PGK-FLPo-IRES-Puro

Plasmid

#205989

Purpose

Expresses Flp recombinase and puromycin resistance

Depositor

Sharon Torigoe

Article

Waite et al PLoS One. 2024 Sep 30;19(9):e0311120. do

Inserts

PGK promoter
FLPo (flp )
IRES-PuroR cassette

Use

Tags

Expression

Mammalian

Mutation

Promoter

PGK promoter

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Opto-GPR152

Plasmid

#106052

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR152; Opto-GPR152; E6)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR152 (GPR152 Human, Bovine)

Use

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Opto-GPR176

Plasmid

#106060

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR176; Opto-GPR176; F2)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR176 (GPR176 Human, Bovine)

Use

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Opto-GPR183

Plasmid

#106062

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR183; Opto-GPR183; F4)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR183 (GPR183 Human, Bovine)

Use

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

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