Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 1,740 results for: plasmid for e coli

Clear keywords
Showing: 251 - 300 of 1740 results

  1. pUC57-BBclone1

    Plasmid
    #137069
    Purpose
    E. coli plasmid that contains the expression optimized genes for BB0405, BB0406, P66, BBA57, HtrA, RevA and BB0238
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Inserts
    BB0405 (BB_0405 Borrelia burgdorferi B31)
    BB0406
    P66
    BBA57
    HtrA
    RevA (revA Borrelia burgdorferi B31)
    BB0238
    Expression
    Bacterial
    Mutation
    wild-type, full-length (signal-peptide-containing…
    Available Since
    March 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    137069-plasmid-map-sequence-id-275695

    Enlarge

  2. pGBW-m4046887

    Plasmid
    #145730
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    April 28, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145730-plasmid-map-sequence-id-292497

    Enlarge

  3. pGBW-m4136464

    Plasmid
    #149534
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    6xHIS;Linker_GGSG;GST;Linker_GGSG;Cleavable TEV
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149534-plasmid-map-sequence-id-307562

    Enlarge

  4. pGBW-m4136461

    Plasmid
    #149538
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    6xHIS;Linker_GGSG;MBP;Linker_GGSG;Cleavable TEV
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149538-plasmid-map-sequence-id-307561

    Enlarge

  5. pGBW-m4046758

    Plasmid
    #145670
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;HA
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145670-plasmid-map-sequence-id-297837

    Enlarge

  6. pGBW-m4046801

    Plasmid
    #145741
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;Myc
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145741-plasmid-map-sequence-id-298377

    Enlarge

  7. pGBW-m4136454

    Plasmid
    #149529
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 M (membrane)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    6xHIS;Linker_GGSG;SUMO tag;Linker_GGSG;Cleavable …
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    May 13, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149529-plasmid-map-sequence-id-297077

    Enlarge

  8. pGBW-m4046931

    Plasmid
    #145706
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;FLAG_2
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145706-plasmid-map-sequence-id-307566

    Enlarge

  9. pGBW-m4136462

    Plasmid
    #149533
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 M (membrane)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    6xHIS;Linker_GGSG;MBP;Linker_GGSG;Cleavable TEV
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149533-plasmid-map-sequence-id-307560

    Enlarge

  10. pGBW-m4046863

    Plasmid
    #145682
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;StrepII
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145682-plasmid-map-sequence-id-298370

    Enlarge

  11. pGBW-m4136457

    Plasmid
    #149537
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 M (membrane)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    6xHIS;Linker_GGSG;GST;Linker_GGSG;Cleavable TEV
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149537-plasmid-map-sequence-id-314686

    Enlarge

  12. pCODE-3

    Plasmid
    #247475
    Purpose
    Plasmid for inducible expression of six tRNA encoding genes (argX, glyT, leuW, proL, argU, and ileX) in Escherichia coli, based on pSEVA121 backbone
    Depositor
    Morten Norholm
    Insert
    argX, glyT, leuW, proL, argU, and ileX
    Expression
    Bacterial
    Mutation
    Rearrangement of tRNA operon, tRNA flanking regio…
    Promoter
    pTet
    Available Since
    Nov. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    247475-plasmid-map-sequence-id-491679

    Enlarge

  13. pHL1914

    Plasmid
    #53042
    Purpose
    PconNoHindM2:hfqlead::gfp, PconNoHind:RBS(st3):tetR
    Depositor
    Han Lim
    Inserts
    GFP
    tetR
    Use
    Synthetic Biology
    Tags
    RBS(st3) and hfqlead
    Expression
    Bacterial
    Promoter
    PconNoHindM2 and pLlacO-1
    Available Since
    May 13, 2014
    Availability
    Academic Institutions and Nonprofits only

  15. pGLU_67

    Plasmid
    #225770
    Purpose
    This is the native promoter from the aph(3')-I kanamycin resistance cassette.
    Depositor
    Nili Ostrov
    Insert
    Paph(3')-I_[abr]
    Use
    Synthetic Biology
    Mutation
    N/A
    Available Since
    Sept. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    225770-plasmid-map-sequence-id-446924

    Enlarge

  16. pTJK368

    Plasmid
    #138006
    Purpose
    BirA* expression. Vector also expresses GFP under control of hUBC promoter
    Depositor
    Tami Kingsbury
    Insert
    BirA*
    Use
    Lentiviral
    Mutation
    None
    Available Since
    May 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    138006-plasmid-map-sequence-id-275000

    Enlarge

  17. pCryptDel4.8

    Plasmid
    #141293
    Purpose
    Plasmid for curing pMUT2 in one step based on pFREE. Contains RelB antitoxin, as well as gRNA targetting pMUT2 plasmid as well as pCryptDel4.8 itself.
    Depositor
    Neel Joshi
    Article
    Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102
    Inserts
    RelB
    gRNA targetting pMUT2
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    Native promoter from pMUT2 relB/relE operon and P…
    Available Since
    Jan. 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    141293-plasmid-map-sequence-id-322822

    Enlarge

  19. pHL1746

    Plasmid
    #53034
    Purpose
    PLtetO-1:ycdTlead::mCherry, PLlacO-1:RBS(st7):csrA
    Depositor
    Han Lim
    Inserts
    mCherry
    csrA
    Use
    Synthetic Biology
    Tags
    RBS(st7) and ycdTlead
    Expression
    Bacterial
    Promoter
    pLlacO-1 and pLtetO-1
    Available Since
    May 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  20. pHL1940

    Plasmid
    #53044
    Purpose
    PLtetO-1:cstA‡, PLlacO-1:RBS(st7):csrA
    Depositor
    Han Lim
    Inserts
    cstA
    csrA
    Use
    Synthetic Biology
    Tags
    RBS(st7)
    Expression
    Bacterial
    Promoter
    pLlacO-1 and pLtetO-1
    Available Since
    Aug. 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  21. pHL1747

    Plasmid
    #53035
    Purpose
    PLtetO-1:ydeHlead::mCherry, PLlacO-1:RBS(st7):csrA
    Depositor
    Han Lim
    Inserts
    mCherry
    csrA
    Use
    Synthetic Biology
    Tags
    RBS(st7) and ydeHlead
    Expression
    Bacterial
    Promoter
    pLlacO-1 and pLtetO-1
    Available Since
    May 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  22. pHL1790

    Plasmid
    #53038
    Purpose
    PLtetO-1:glgClead::mCherry, PLlacO-1:csrB
    Depositor
    Han Lim
    Inserts
    mCherry
    csrB
    Use
    Synthetic Biology
    Tags
    glgC leader
    Expression
    Bacterial
    Promoter
    pLlacO-1 and pLtetO-1
    Available Since
    May 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  23. pHL1745

    Plasmid
    #53033
    Purpose
    PLtetO-1:cstAlead::mCherry, PLlacO-1:RBS(st7):csrA
    Depositor
    Han Lim
    Inserts
    mCherry
    csrA
    Use
    Synthetic Biology
    Tags
    RBS(st7) and cstAlead
    Expression
    Bacterial
    Promoter
    pLlacO-1 and pLtetO-1
    Available Since
    May 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  24. pHL1724

    Plasmid
    #53031
    Purpose
    PLtetO-1:pgaAleadFull::mCherry, PLlacO-1:RBS(st7):csrA
    Depositor
    Han Lim
    Inserts
    mCherry
    csrA
    Use
    Synthetic Biology
    Tags
    RBS(st7) and pgaAleadFull
    Expression
    Bacterial
    Promoter
    pLlacO-1 and pLtetO-1
    Available Since
    May 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  25. pHL1928

    Plasmid
    #53043
    Purpose
    PLtetO-1:Agn43RBS:mCherry, PLlacO-1:RBS(st7):csrA
    Depositor
    Han Lim
    Inserts
    mCherry
    csrA
    Use
    Synthetic Biology
    Tags
    Agn43RBS and RBS(st7)
    Expression
    Bacterial
    Promoter
    pLlacO-1 and pLtetO-1
    Available Since
    May 13, 2014
    Availability
    Academic Institutions and Nonprofits only

  27. pGBW-m4136458

    Plasmid
    #149531
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    6xHIS;Linker_GGSG;SUMO tag;Linker_GGSG;Cleavable …
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    May 13, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149531-plasmid-map-sequence-id-297078

    Enlarge

  28. L5-LexAp65-L2

    Plasmid
    #41437
    Depositor
    Steve Stowers
    Insert
    LexAp65
    Expression
    Bacterial
    Available Since
    Dec. 6, 2012
    Availability
    Academic Institutions and Nonprofits only
    41437-plasmid-map-sequence-id-55247

    Enlarge

  29. R4-LexAp65-R3

    Plasmid
    #41438
    Depositor
    Steve Stowers
    Insert
    LexAp65
    Expression
    Bacterial
    Available Since
    Nov. 29, 2012
    Availability
    Academic Institutions and Nonprofits only
    41438-plasmid-map-sequence-id-55248

    Enlarge

  30. pGLU_103

    Plasmid
    #225785
    Purpose
    This is the promoter region taken from upstream of Tn5 transposase in pBAM1. It can be considered the "default" promoter to use.
    Depositor
    Nili Ostrov
    Insert
    Ptn5_[Tn5]
    Use
    Synthetic Biology
    Mutation
    N/A
    Available Since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    225785-plasmid-map-sequence-id-446997

    Enlarge

  31. pGLU_85

    Plasmid
    #225800
    Purpose
    This is the promoter region taken from upstream of Himar transposase in pMarC9-Kan. It can be considered the "default" promoter to use.
    Depositor
    Nili Ostrov
    Insert
    PmarC9_[Himar]
    Use
    Synthetic Biology
    Mutation
    N/A
    Available Since
    Sept. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    225800-plasmid-map-sequence-id-446917

    Enlarge

  32. pSIMcpf1

    Plasmid
    #153034
    Purpose
    For CRISPR-Cas12a genome editing in E. coli. Contains; constituitively expressed Ascpf1 gene, a heat-inducible lambda red recombination system, an arabinose-inducible CRISPR array targeting pMB1.
    Depositor
    Nigel Scrutton
    Article
    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.
    Insert
    Ascpf1
    Expression
    Bacterial
    Available Since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    153034-plasmid-map-sequence-id-320039

    Enlarge

  33. pTF-lacZ

    Plasmid
    #153036
    Purpose
    For CRISPR-Cas12a genome editing in E. coli for the insertion of large DNA fragments at the lacZ locus. Contains a constitutively expressed CRISPR array targeting the lacZ gene . No cargo DNA.
    Depositor
    Nigel Scrutton
    Article
    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.
    Insert
    CRISPR array
    Expression
    Bacterial
    Available Since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    153036-plasmid-map-sequence-id-318836

    Enlarge

Showing: 251 - 300 of 1740 results