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We narrowed to 1,640 results for: plasmid for e coli

Showing: 251 - 300 of 1640 results

  1. pGBW-m4046801

    Plasmid
    #145741
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;Myc
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145741-plasmid-map-sequence-id-298377

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  2. pGBW-m4136454

    Plasmid
    #149529
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 M (membrane)
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    6xHIS;Linker_GGSG;SUMO tag;Linker_GGSG;Cleavable …
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    May 13, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149529-plasmid-map-sequence-id-297077

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  3. pGBW-m4046931

    Plasmid
    #145706
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;FLAG_2
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145706-plasmid-map-sequence-id-307566

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  4. pGBW-m4136462

    Plasmid
    #149533
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 M (membrane)
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    6xHIS;Linker_GGSG;MBP;Linker_GGSG;Cleavable TEV
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149533-plasmid-map-sequence-id-307560

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  5. pGBW-m4046863

    Plasmid
    #145682
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;StrepII
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145682-plasmid-map-sequence-id-298370

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  6. pGBW-m4136457

    Plasmid
    #149537
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 M (membrane)
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    6xHIS;Linker_GGSG;GST;Linker_GGSG;Cleavable TEV
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149537-plasmid-map-sequence-id-314686

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  7. pHL1914

    Plasmid
    #53042
    Purpose
    PconNoHindM2:hfqlead::gfp, PconNoHind:RBS(st3):tetR
    Depositor
    Han Lim
    Inserts
    GFP
    tetR
    Use
    Synthetic Biology
    Tags
    RBS(st3) and hfqlead
    Expression
    Bacterial
    Mutation
    Promoter
    PconNoHindM2 and pLlacO-1
    Available since
    May 13, 2014
    Availability
    Academic Institutions and Nonprofits only

  9. pGLU_67

    Plasmid
    #225770
    Purpose
    This is the native promoter from the aph(3')-I kanamycin resistance cassette.
    Depositor
    Nili Ostrov
    Insert
    Paph(3')-I_[abr]
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    N/A
    Promoter
    Available since
    Sept. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    225770-plasmid-map-sequence-id-446924

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  10. pTJK368

    Plasmid
    #138006
    Purpose
    BirA* expression. Vector also expresses GFP under control of hUBC promoter
    Depositor
    Tami Kingsbury
    Insert
    BirA*
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    May 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    138006-plasmid-map-sequence-id-275000

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  11. pCryptDel4.8

    Plasmid
    #141293
    Purpose
    Plasmid for curing pMUT2 in one step based on pFREE. Contains RelB antitoxin, as well as gRNA targetting pMUT2 plasmid as well as pCryptDel4.8 itself.
    Depositor
    Neel Joshi
    Article
    Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102
    Inserts
    RelB
    gRNA targetting pMUT2
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Native promoter from pMUT2 relB/relE operon and P…
    Available since
    Jan. 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    141293-plasmid-map-sequence-id-322822

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  12. pMis1_1B

    Plasmid
    #192079
    Purpose
    A pBBR-1 based vector with IncP group plasmid compatibility for expression in Methylorubrum extorquens using rhamnose dependent induction
    Depositor
    Markus Buchhaupt
    Article
    Poschel et al Microbiologyopen. 2022 Oct;11(5):e1325.
    Inserts
    KanR
    rhaR
    rhaS
    pBBR1 rep
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    192079-plasmid-map-sequence-id-380008

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  13. pHL1746

    Plasmid
    #53034
    Purpose
    PLtetO-1:ycdTlead::mCherry, PLlacO-1:RBS(st7):csrA
    Depositor
    Han Lim
    Inserts
    mCherry
    csrA
    Use
    Synthetic Biology
    Tags
    RBS(st7) and ycdTlead
    Expression
    Bacterial
    Mutation
    Promoter
    pLlacO-1 and pLtetO-1
    Available since
    May 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  14. pHL1940

    Plasmid
    #53044
    Purpose
    PLtetO-1:cstA‡, PLlacO-1:RBS(st7):csrA
    Depositor
    Han Lim
    Inserts
    cstA
    csrA
    Use
    Synthetic Biology
    Tags
    RBS(st7)
    Expression
    Bacterial
    Mutation
    Promoter
    pLlacO-1 and pLtetO-1
    Available since
    Aug. 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  15. pHL1747

    Plasmid
    #53035
    Purpose
    PLtetO-1:ydeHlead::mCherry, PLlacO-1:RBS(st7):csrA
    Depositor
    Han Lim
    Inserts
    mCherry
    csrA
    Use
    Synthetic Biology
    Tags
    RBS(st7) and ydeHlead
    Expression
    Bacterial
    Mutation
    Promoter
    pLlacO-1 and pLtetO-1
    Available since
    May 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  16. pHL1790

    Plasmid
    #53038
    Purpose
    PLtetO-1:glgClead::mCherry, PLlacO-1:csrB
    Depositor
    Han Lim
    Inserts
    mCherry
    csrB
    Use
    Synthetic Biology
    Tags
    glgC leader
    Expression
    Bacterial
    Mutation
    Promoter
    pLlacO-1 and pLtetO-1
    Available since
    May 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  17. pHL1745

    Plasmid
    #53033
    Purpose
    PLtetO-1:cstAlead::mCherry, PLlacO-1:RBS(st7):csrA
    Depositor
    Han Lim
    Inserts
    mCherry
    csrA
    Use
    Synthetic Biology
    Tags
    RBS(st7) and cstAlead
    Expression
    Bacterial
    Mutation
    Promoter
    pLlacO-1 and pLtetO-1
    Available since
    May 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  18. pHL1724

    Plasmid
    #53031
    Purpose
    PLtetO-1:pgaAleadFull::mCherry, PLlacO-1:RBS(st7):csrA
    Depositor
    Han Lim
    Inserts
    mCherry
    csrA
    Use
    Synthetic Biology
    Tags
    RBS(st7) and pgaAleadFull
    Expression
    Bacterial
    Mutation
    Promoter
    pLlacO-1 and pLtetO-1
    Available since
    May 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  19. pHL1928

    Plasmid
    #53043
    Purpose
    PLtetO-1:Agn43RBS:mCherry, PLlacO-1:RBS(st7):csrA
    Depositor
    Han Lim
    Inserts
    mCherry
    csrA
    Use
    Synthetic Biology
    Tags
    Agn43RBS and RBS(st7)
    Expression
    Bacterial
    Mutation
    Promoter
    pLlacO-1 and pLtetO-1
    Available since
    May 13, 2014
    Availability
    Academic Institutions and Nonprofits only

  21. pGBW-m4136458

    Plasmid
    #149531
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    6xHIS;Linker_GGSG;SUMO tag;Linker_GGSG;Cleavable …
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    May 13, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149531-plasmid-map-sequence-id-297078

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  22. L5-LexAp65-L2

    Plasmid
    #41437
    Depositor
    Steve Stowers
    Insert
    LexAp65
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 6, 2012
    Availability
    Academic Institutions and Nonprofits only
    41437-plasmid-map-sequence-id-55247

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  23. R4-LexAp65-R3

    Plasmid
    #41438
    Depositor
    Steve Stowers
    Insert
    LexAp65
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 29, 2012
    Availability
    Academic Institutions and Nonprofits only
    41438-plasmid-map-sequence-id-55248

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  24. pGLU_103

    Plasmid
    #225785
    Purpose
    This is the promoter region taken from upstream of Tn5 transposase in pBAM1. It can be considered the "default" promoter to use.
    Depositor
    Nili Ostrov
    Insert
    Ptn5_[Tn5]
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    N/A
    Promoter
    Available since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    225785-plasmid-map-sequence-id-446997

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  25. pGLU_85

    Plasmid
    #225800
    Purpose
    This is the promoter region taken from upstream of Himar transposase in pMarC9-Kan. It can be considered the "default" promoter to use.
    Depositor
    Nili Ostrov
    Insert
    PmarC9_[Himar]
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    N/A
    Promoter
    Available since
    Sept. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    225800-plasmid-map-sequence-id-446917

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  26. pSIMcpf1

    Plasmid
    #153034
    Purpose
    For CRISPR-Cas12a genome editing in E. coli. Contains; constituitively expressed Ascpf1 gene, a heat-inducible lambda red recombination system, an arabinose-inducible CRISPR array targeting pMB1.
    Depositor
    Nigel Scrutton
    Article
    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.
    Insert
    Ascpf1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    153034-plasmid-map-sequence-id-320039

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  27. pTF-lacZ

    Plasmid
    #153036
    Purpose
    For CRISPR-Cas12a genome editing in E. coli for the insertion of large DNA fragments at the lacZ locus. Contains a constitutively expressed CRISPR array targeting the lacZ gene . No cargo DNA.
    Depositor
    Nigel Scrutton
    Article
    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.
    Insert
    CRISPR array
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    153036-plasmid-map-sequence-id-318836

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  45. pMK110

    Plasmid
    #99535
    Purpose
    contains bgl operon sequence for E. coli in vitro transcription template
    Depositor
    Robert Landick
    Article
    Kotlajich et al Elife. 2015 Jan 16;4. doi: 10.7554/eLife
    Insert
    E. coli bgl antisense region containing bglG
    Use
    Tags
    None
    Expression
    Mutation
    Promoter
    lambda Pr promoter used for in vitro transcriptio…
    Available since
    Aug. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    99535-plasmid-map-sequence-id-190923

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  46. pCMV-TALEN-Rep

    Plasmid
    #45964
    Depositor
    Ralf Kuehn
    Insert
    beta-Galactosidase
    Use
    Tags
    Expression
    Mammalian
    Mutation
    duplicated aa 1-135
    Promoter
    CMV
    Available since
    Sept. 13, 2013
    Availability
    Academic Institutions and Nonprofits only
    45964-plasmid-map-sequence-id-64559

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  47. pCMV-Rab-Rep

    Plasmid
    #45967
    Depositor
    Ralf Kuehn
    Insert
    beta-Galactosidase
    Use
    Tags
    Expression
    Mammalian
    Mutation
    duplicated aa 1-135
    Promoter
    CMV
    Available since
    Sept. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    45967-plasmid-map-sequence-id-64561

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  50. PHB1

    Plasmid
    #212153
    Purpose
    For PHB production in E. coli (PT7-pMB1).
    Depositor
    Kang Zhou
    Insert
    poly(3-hydroxyalkanoate) polymerase subunit PhaC, PHA-specific beta-ketothiolase, acetoacetyl-CoA reductase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PT7
    Available since
    Jan. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    212153-plasmid-map-sequence-id-420764

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Showing: 251 - 300 of 1640 results