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pET151-HIS-TEV-GST-PP-RPN13

Plasmid

#73741

Purpose

Expression of hRPN13 in E.coli

Depositor

Chris Hill

Article

VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

Insert

RPN13 (ADRM1 Human)

Use

Tags

6xHIS and GST

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 27, 2017

Availability

Academic Institutions and Nonprofits only
pCAG-OSF-PP-human-CHMP3(150)

Plasmid

#154181

Purpose

expresses human CHMP3(150) in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

CHMP3 (CHMP3 Human)

Use

Tags

FLAG and Strep-Tag II

Expression

Mammalian

Mutation

C-terminal truncation of CHMP3 at amino acid 150

Promoter

CAG

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pET28-Mff(1-61)-PP-GST

Plasmid

#73042

Purpose

Bacterial expression of mouse Mff residues 1-61 with C-terminal GST-tag and PreScission protease cleavage site

Depositor

David Chan

Article

Liu et al Mol Biol Cell. 2015 Dec 1;26(24):4466-77

Insert

Mff(1-61) (Mff Mouse)

Use

Tags

GST-tag and PreScission protease site

Expression

Bacterial

Mutation

Truncation containing amino acids 1-61

Promoter

T7

Available since

Feb. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pF(UG) hSyn PP-HaloTag-SYT7alpha

Plasmid

#179714

Purpose

Lentiviral expression of mouse Syt7 with N terminal HaloTag

Depositor

Edwin Chapman

Article

Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

Insert

SYT7 (Syt7 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-PP-Spastin(87-616)DeltaExon4

Plasmid

#128794

Purpose

Bacteiral expression of Spastin M87 varient without exon 4

Depositor

Chris Hill

Article

Han et al J Biol Chem. 2019 Nov 25. pii: AC119.009

Insert

Spastin (SPAST Human)

Use

`

Tags

GST

Expression

Bacterial

Mutation

residues 87-616 without exon 4.

Promoter

T7

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pET151-GST-PP-RPN13 (19-132)

Plasmid

#73742

Purpose

Expression of human RPN13 PRU domain in E.coli

Depositor

Chris Hill

Article

VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

Insert

RPN13 (ADRM1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

PRU domain only (residues 19-132)

Promoter

T7

Available since

April 27, 2017

Availability

Academic Institutions and Nonprofits only
pET151-GFP-PP-RPN2 (925-953)

Plasmid

#73744

Purpose

Expression of GFP-PP-RPN2 (925-953) fusion in E.coli

Depositor

Chris Hill

Article

VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

Insert

RPN2 (RPN2 Human)

Use

Tags

6x His and GFP

Expression

Bacterial

Mutation

Construct possesses only residues 932-953 of RPN2

Promoter

T7

Available since

April 27, 2017

Availability

Academic Institutions and Nonprofits only
pET151-GFP-PP-RPN2 (940-953)

Plasmid

#73746

Purpose

Expression of GFP-PP-RPN2 (940-953) fusion in E.coli

Depositor

Chris Hill

Article

VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

Insert

RPN2 (RPN2 Human)

Use

Tags

6x His and GFP

Expression

Bacterial

Mutation

Construct possesses only residues 940-953 of RPN2

Promoter

T7

Available since

April 27, 2017

Availability

Academic Institutions and Nonprofits only
pET151-GFP-PP-RPN2 (918-932)

Plasmid

#73748

Purpose

Expression of GFP-PP-RPN2 (918-932) fusion in E.coli

Depositor

Chris Hill

Article

VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

Insert

RPN2 (RPN2 Human)

Use

Tags

6x His and GFP

Expression

Bacterial

Mutation

Construct possesses only residues 918-932 of RPN2

Promoter

T7

Available since

April 27, 2017

Availability

Academic Institutions and Nonprofits only
pCAG-OSF-PP-squirrel-monkey-CHMP3

Plasmid

#154182

Purpose

expresses squirrel monkey CHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

CHMP3

Use

Tags

FLAG, Strep-Tag II, and preScission site

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-OSF-PP-squirrel-monkey-retroCHMP3

Plasmid

#154184

Purpose

expresses squirrel monkey retroCHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

retroCHMP3

Use

Tags

FLAG, Strep-Tag II, and preScission site

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-PP-NEDD4L-WW1(193-226)

Plasmid

#166701

Purpose

expresses NEDD4L WW1 in bacterial cells

Depositor

Wesley Sundquist

Article

Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

Insert

NEDD4L WW1 domain

Use

Tags

GST, HRV 3C site, and Thrombin site

Expression

Bacterial

Mutation

Promoter

tac promoter

Available since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-PP-NEDD4L-WW2(385-418)

Plasmid

#166702

Purpose

expresses NEDD4L WW2 in bacterial cells

Depositor

Wesley Sundquist

Article

Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

Insert

NEDD4L WW2 domain

Use

Tags

GST, HRV 3C site, and Thrombin site

Expression

Bacterial

Mutation

Promoter

tac promoter

Available since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-PP-NEDD4L-WW3(497-530)

Plasmid

#166703

Purpose

expresses NEDD4L WW3 in bacterial cells

Depositor

Wesley Sundquist

Article

Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

Insert

NEDD4L WW3 domain

Use

Tags

GST, HRV 3C site, and Thrombin site

Expression

Bacterial

Mutation

Promoter

tac promoter

Available since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-PP-NEDD4L-WW4(548-581)

Plasmid

#166704

Purpose

expresses NEDD4L WW4 in bacterial cells

Depositor

Wesley Sundquist

Article

Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

Insert

NEDD4L WW4 domain

Use

Tags

GST, HRV 3C site, and Thrombin site

Expression

Bacterial

Mutation

Promoter

tac promoter

Available since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET151-GFP-PP-RPN2 (918-953)

Plasmid

#73743

Purpose

Expression of GFP-PP-RPN2 (918-53) fusion in E.coli

Depositor

Chris Hill

Article

VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

Insert

RPN2 (RPN2 Human)

Use

Tags

6x His and GFP

Expression

Bacterial

Mutation

Construct possesses only residues 918-953 of RPN2

Promoter

T7

Available since

April 27, 2017

Availability

Academic Institutions and Nonprofits only
pET151-GFP-PP-RPN2 (932-953)

Plasmid

#73745

Purpose

Expression of GFP-PP-RPN2 (932-953) fusion in E.coli

Depositor

Chris Hill

Article

VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

Insert

RPN2 (RPN2 Human)

Use

Tags

6x His and GFP

Expression

Bacterial

Mutation

Construct possesses only residues 932-953 of RPN2

Promoter

T7

Available since

April 27, 2017

Availability

Academic Institutions and Nonprofits only
pET151-GFP-PP-RPN2 (918-947)

Plasmid

#73747

Purpose

Expression of GFP-PP-RPN2 (918-947) fusion in E.coli

Depositor

Chris Hill

Article

VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

Insert

RPN2 (RPN2 Human)

Use

Tags

6x His and GFP

Expression

Bacterial

Mutation

Construct possesses only residues 918-947 of RPN2

Promoter

T7

Available since

April 27, 2017

Availability

Academic Institutions and Nonprofits only
pET151-GFP-PP-RPN2 (797-953)

Plasmid

#74228

Purpose

[Edit] Expression of GFP-PP-RPN2 (797-953) fusion in E.coli

Depositor

Chris Hill

Article

VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

Insert

RPN2 (RPN2 Human)

Use

Tags

6x His and GFP

Expression

Bacterial

Mutation

Construct possesses only residues 918-953 of RPN2

Promoter

T7

Available since

April 27, 2017

Availability

Academic Institutions and Nonprofits only
pCAG-OSF-PP-squirrel-monkey-CHMP3(155)

Plasmid

#154183

Purpose

expresses squirrel monkey CHMP3(155) in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

CHMP3(155)

Use

Tags

FLAG, Strep-Tag II, and preScission site

Expression

Mammalian

Mutation

C-terminal truncation of CHMP3 at amino acid 155

Promoter

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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