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Showing: 101 - 120 of 2010 results

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pEntry_HCoV-HKU1-nsp6

Plasmid

#168895

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-HKU1-nsp6 (ORF1ab )

Use

Gateway-compatible entry vector

Tags

Expression

Mutation

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEntry_SARS-CoV-1-nsp6

Plasmid

#168848

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

SARS-CoV-1-nsp6 (ORF1ab )

Use

Gateway-compatible entry vector

Tags

Expression

Mutation

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEntry_MERS-CoV-nsp6

Plasmid

#168819

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

MERS-CoV-nsp6 (orf1ab )

Use

Gateway-compatible entry vector

Tags

Expression

Mutation

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pSP64T-ythdf1-3xflag

Plasmid

#164488

Purpose

Expresses zebrafish ythdf1 with 3x flag tag, for mRNA injection

Depositor

Antonio Giraldez

Article

Kontur et al Cell Rep. 2020 Dec 29;33(13):108598. doi

Insert

ythdf1 (ythdf1 Zebrafish)

Use

Production of mrna for zebrafish injection.

Tags

3x-flagtag

Expression

Mutation

Promoter

SP6

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSP64T-ythdf3-3xflag

Plasmid

#164490

Purpose

Expresses zebrafish ythdf3 with 3x flag tag, for mRNA injection

Depositor

Antonio Giraldez

Article

Kontur et al Cell Rep. 2020 Dec 29;33(13):108598. doi

Insert

ythdf3 (ythdf3 Zebrafish)

Use

Production of mrna for zebrafish injection.

Tags

3x-flagtag

Expression

Mutation

Promoter

SP6

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSP64-hERG1a D540K

Plasmid

#53057

Purpose

Expresses alpha subunit of D540K hERG1a K channel in Xenopus oocytes

Depositor

Michael Sanguinetti

Article

Sanguinetti et al J Physiol. 1999 Feb 1;514 ( Pt 3):667-75

Insert

Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)

Use

Xenopus oocyte expression

Tags

Expression

Mutation

changed Aspartate 540 to Lysine

Promoter

SP6

Available since

Oct. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pSP64-hERG1a S620T

Plasmid

#53059

Purpose

Expresses alpha subunit of S620T hERG1a K channel in Xenopus oocytes

Depositor

Michael Sanguinetti

Article

Casis et al Mol Pharmacol. 2006 Feb;69(2):658-65. Ep

Insert

Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)

Use

Xenopus oocyte expression

Tags

Expression

Mutation

changed Serine 620 to Threonine

Promoter

SP6

Available since

June 9, 2014

Availability

Academic Institutions and Nonprofits only

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pSP64-hERG1a S631A

Plasmid

#53058

Purpose

Expresses alpha subunit of S631A hERG1a K channel in Xenopus oocytes

Depositor

Michael Sanguinetti

Article

Zou et al J Physiol. 1998 May 15;509 ( Pt 1):129-3

Insert

Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)

Use

Xenopus oocyte expression

Tags

Expression

Mutation

changed Serine 631 to Alanine

Promoter

SP6

Available since

June 9, 2014

Availability

Academic Institutions and Nonprofits only

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HOXB4-FLAG-pSP65

Plasmid

#20990

Depositor

Corey Largman

Article

Chang et al Genes Dev. 1995 Mar 15. 9(6):663-74.

Insert

HOXB4 (HOXB4 Human)

Use

In vitro trans/trans

Tags

Expression

Mutation

Missing first 14 amino acids

Promoter

Available since

Nov. 10, 2009

Availability

Academic Institutions and Nonprofits only
HOXB6-FLAGA-pSP65

Plasmid

#20994

Depositor

Corey Largman

Article

Shen et al Nucleic Acids Res. 1996 Mar 1;24(5):898-

Insert

HOXB6 (HOXB6 Human)

Use

In vitro trans/trans

Tags

FLAG

Expression

Mutation

Promoter

Available since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
pSP64T XAR1 (DM#105)

Plasmid

#15044

Depositor

Douglas Melton

Article

Hemmati-Brivanlou et al Nature. 1992 Oct 15. 359(6396):609-14.

Insert

Xenopus activin receptor 1 (xstk2 Frog)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only
pSP64TNE Xnr4 (DM#174)

Plasmid

#15078

Depositor

Douglas Melton

Article

Joseph et al Dev Biol. 1997 Apr 15. 184(2):367-72.

Insert

Xnr4 (xnr4 Frog)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only
pBR9-genomic_RSP6-HAX3

Plasmid

#176703

Purpose

Rescue of RSP6 Chlamydomonas mutant with tagged RSP6

Depositor

Ron Vale

Article

Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

Insert

RSP6

Use

Tags

HAX3

Expression

Plant

Mutation

None

Promoter

rbcs2

Available since

Availability

Academic Institutions and Nonprofits only

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PCR4-Hsp68::lacZ-H11

Plasmid

#139099

Purpose

enSERT LacZ reporter vector for site-specific integration into the H11 locus (contains Hsp68 minimal promoter)

Depositor

Len Pennacchio

Article

Kvon et al Cell. 2020 Mar 6. pii: S0092-8674(20)302

Type

Empty backbone

Use

CRISPR and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

Hsp68

Available since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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PCR4-Hsp68::eGFP-H11

Plasmid

#211941

Purpose

dual-enSERT-1 eGFP vector for site-specific integration into the H11 locus (contains hsp68 minimal promoter)

Depositor

Evgeny Kvon

Article

Hollingsworth et al bioRxiv 2023.12.10.570890

Type

Empty backbone

Use

CRISPR and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

HSP68

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pSP64-GFP-ePDZ-CAAX

Plasmid

#135603

Purpose

Expression of GFP-ePDZ-CAAX mRNA for microinjection to be used for optogenetics in S. purpuratus

Depositor

Mamiko Yajima

Article

Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

Insert

Lifeact-GFP-ePDZ-CAAX

Use

In vitro transcription (mrna synthesis)

Tags

CAAX, EGFP, and ePDZ

Expression

Mutation

Promoter

Unknown

Available since

Jan. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pSP64-membrane-mCherry-LOV

Plasmid

#135599

Purpose

Expression of membrane-mCherry-LOV mRNA for microinjection for use of optogenetics in S. purpuratus

Depositor

Mamiko Yajima

Article

Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

Insert

membrane-mCherry-LOV

Use

In vitro transcription (mrna synthesis)

Tags

LOV and mCherry

Expression

Mutation

Promoter

Unknown

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV-2 NSP6

Plasmid

#141260

Purpose

Gateway-compatible Entry vector, with insert of NSP6 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP6 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway-compatible entry vector

Tags

Expression

Mutation

Many synonymous changes due to codon optimization

Promoter

Available since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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aav-hsp68-mCherry-MCS-pA

Plasmid

#195412

Purpose

AAV9 based MPRA assay

Depositor

William Pu

Article

Akerberg et al Nat Commun. 2019 Oct 28;10(1):4907. doi:

Type

Empty backbone

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

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HOXB7 (human) FLAG pSP65

Plasmid

#8537

Depositor

Corey Largman

Article

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB7 (HOXB7 Human)

Use

In vitro transcription/translation

Tags

FLAG

Expression

Bacterial

Mutation

starts at AA#1 preceeded by FLAG and Ser-Arg-Ile…

Promoter

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only