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WT GLUT1-GFP

Plasmid

#200093

Purpose

WT human GLUT1 with a C-terminal GFP tag in pQCXIP vector

Depositor

Jason MacGurn

Article

Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

Insert

GLUT1 (SLC2A1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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pLJM1-Flag-Rheb

Plasmid

#165031

Purpose

expression of Flag tagged Rheb protein in mammalian cells

Depositor

Ken Inoki

Article

Yao et al Mol Cell. 2020 Nov 5;80(3):437-451.e6. d

Insert

Rheb (RHEB Human)

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

Promoter

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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PXPR007 sgGFP-1

Plasmid

#74960

Purpose

Cas9 + sgGFP-1 with blasticidin selection

Depositor

William Hahn

Article

Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

Insert

GFP

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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PXPR007 sgGFP-2

Plasmid

#74961

Purpose

Cas9 + sgGFP-2 with blasticidin selection

Depositor

William Hahn

Article

Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

Insert

GFP

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003 sgLacZ-1

Plasmid

#74968

Purpose

sgLacZ-1, puromycin selection

Depositor

William Hahn

Article

Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

Insert

LacZ

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003 sgLacZ-2

Plasmid

#74969

Purpose

sgLacZ-2, puromycin selection

Depositor

William Hahn

Article

Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

Insert

LacZ

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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AAV-RO1.7-dnHIF

Plasmid

#206343

Purpose

AAV plasmid expressing dominant negative HIF1A in photoreceptors

Depositor

Connie Cepko

Article

Xue et al Elife. 2021 Apr 13;10. pii: 66240. doi:

Insert

dominant negative hif1a

Use

AAV

Tags

Expression

Mutation

dominant negative HIF1A

Promoter

1.7kb human red opsin

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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1K245-->R GLUT1-GFP

Plasmid

#200095

Purpose

1K245--> GLUT1 mutant with C-terminal GFP tag in pQCXIP vector

Depositor

Jason MacGurn

Article

Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

Insert

GLUT1 (SLC2A1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

K245R

Promoter

CMV

Available since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
5K-tails-->R GLUT1-GFP

Plasmid

#200099

Purpose

human GLUT1 with K-->R mutation of 5 lysines on the N- and C-terminal tails with a C-terminal tag in pQCXIP vector

Depositor

Jason MacGurn

Article

Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

Insert

GLUT1 (SLC2A1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

K-->R mutations of amino acids 6, 7, 451, 456,…

Promoter

CMV

Available since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
TXD13-CD4d3+4-bio

Plasmid

#73108

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding TXD13 with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

TXD13 (TMX4 Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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6K-loop-->R GLUT1-GFP

Plasmid

#200098

Purpose

human GLUT1 with K-->R mutation of 6 lysines in the major cytoplasmic loop and C-terminal GFP tag in pQCXIP vector

Depositor

Jason MacGurn

Article

Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

Insert

GLUT1 (SLC2A1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

K-->R mutations of amino acids 225, 229, 230, …

Promoter

CMV

Available since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
11K-cyto-->R GLUT1-GFP

Plasmid

#200096

Purpose

human GLUT1 with K-->R mutations for all 11 cytosolic lysines and a C-terminal GFP tag in pQCXIP vector

Depositor

Jason MacGurn

Article

Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

Insert

GLUT1 (SLC2A1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

K-->R mutations of amino acids 6, 7, 225, 229,…

Promoter

CMV

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
1K245 GLUT1-GFP

Plasmid

#200097

Purpose

human GLUT1 with a single cytosolic lysine (K245) and C-terminal GFP tag in pQCXIP vector

Depositor

Jason MacGurn

Article

Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

Insert

GLUT1 (SLC2A1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

K-->R mutations of amino acids 6, 7, 225, 229,…

Promoter

CMV

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-RO1.7-Hsp90ab1-FLAG

Plasmid

#206355

Purpose

AAV plasmid expressing FLAG-tagged HSP90AB1 in photoreceptors

Depositor

Connie Cepko

Article

Xue et al Elife. 2024 May 10;12:RP90749. doi: 10.7

Insert

Hsp90ab1 (Hsp90ab1 Mouse)

Use

AAV

Tags

3xFLAG

Expression

Mutation

Promoter

1.7kb human red opsin

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pLX_TRC311 ETV4-L

Plasmid

#74982

Purpose

Constitutive ETV4 ORF, blasticidin selection

Depositor

William Hahn

Article

Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

Insert

ETV4 (ETV4 Human)

Use

Tags

Expression

Mammalian

Mutation

S396N

Promoter

EF1a

Available since

July 7, 2016

Availability

Academic Institutions and Nonprofits only

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pLX_TRC311 ETV4-S

Plasmid

#74983

Purpose

Constitutive ETV4 ORF, blasticidin selection

Depositor

William Hahn

Article

Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

Insert

ETV4 (ETV4 )

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLJM1-Flag-Rheb-4Rs

Plasmid

#165022

Purpose

expression of Flag-Rheb-4Rs mutant protein in mammalian cells (the lysine residues on the position 109, 135, 151 and 178 on Rheb protein are mutated to arginines)

Depositor

Ken Inoki

Article

Yao et al Mol Cell. 2020 Nov 5;80(3):437-451.e6. d

Insert

Rheb-4Rs (RHEB Human)

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

change lysine residues on position 109, 135, 151,…

Promoter

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-TRSter

Plasmid

#78865

Purpose

Expresses rat Thioredoxin Reductase using an open reading frame in fusion with a mutated E. coli-type SECIS element

Depositor

Elias Arnér

Article

Arner et al J Mol Biol. 1999 Oct 8;292(5):1003-16.

Insert

Thioredoxin Reductase (Txnrd1 Rat)

Use

Tags

Mutated E. coli-type SECIS element (3’ end of ins…

Expression

Bacterial

Mutation

Promoter

T7lac

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pLX_TRC311 ETV1

Plasmid

#74981

Purpose

Constitutive ETV1 ORF, blasticidin selection

Depositor

William Hahn

Article

Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

Insert

ETV1 (ETV1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
PXPR007 sgCIC-2

Plasmid

#74959

Purpose

Cas9 + sgCIC-2 with blasticidin selection

Depositor

William Hahn

Article

Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

Insert

CIC (CIC Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge