We narrowed to 9,223 results for: Coli

1. pCT02

   Plasmid

   #212152

   Purpose

   Plasmid expressing 6x His tagged FAST-PETase under inducible promoter PT7 control.

   Depositor

   Kang Zhou

   Insert

   FAST-PETase
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   S121E, D186H, R224Q, N233K, R280A

   Promoter

   PT7

   Available since

   March 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCas9CR5-NG

   Plasmid

   #120228

   Purpose

   pCas9CR5 with mutations to change PAM site specificity to NG from Nishimasu et al.

   Depositor

   Christopher Reisch

   Insert

   SpCas9-NG
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Mutations R1335V/L1111R/D1135V/G1218R/E1219F/A132…

   Promoter

   pTet

   Available since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKM154

   Plasmid

   #13036

   Depositor

   Kenan Murphy

   Article

   Murphy et al Gene. 2000 Apr 4. 246(1-2):321-30.

   Insert

   cat, sacB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 7, 2006

   Availability

   Academic Institutions and Nonprofits only

4. pExp-PpiB

   Plasmid

   #112572

   Purpose

   Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and PpiB (peptidyl-prolyl cis-trans isomerase B from E. coli) fusions with optional C-terminal StrepII-tag

   Depositor

   Marko Hyvönen

   Type

   Empty backbone

   Use

   Tags

   8xHis-PpiB and Strep tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCas9CR4-NG

   Plasmid

   #120225

   Purpose

   pCas9CR4 with mutations to change PAM site specificity to NG from Nishimasu et al.

   Depositor

   Christopher Reisch

   Insert

   SpCas9-NG
   

   Use

   Synthetic Biology

   Tags

   ssrA

   Expression

   Mutation

   Mutations R1335V/L1111R/D1135V/G1218R/E1219F/A132…

   Promoter

   pTet

   Available since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET mRuby2 LIC cloning vector (H6-mRuby2)

   Plasmid

   #86934

   Purpose

   pET LIC cloning vector for Biotin-His6-tev-yORF-mRuby2

   Depositor

   Chris Jeans

   Type

   Empty backbone

   Use

   Tags

   6xHis, AviTag (biotin), TEV cleavage site, and mR…

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available since

   March 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEB355

   Plasmid

   #22070

   Depositor

   Emmanuelle Bouveret

   Article

   Gully et al Proteomics. 2006 Jan . 6(1):282-93.

   Type

   Empty backbone

   Use

   Tags

   T18

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pExp-DsbA

   Plasmid

   #112562

   Purpose

   Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and DsbA (leaderless thiol:disulfide interchange protein DsbA from E. coli) fusions with optional C-terminal StrepII-tag

   Depositor

   Marko Hyvönen

   Type

   Empty backbone

   Use

   Tags

   8xHis-DsbA(AA20-208) and Strep tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pExp-DsbA(SS)

   Plasmid

   #112563

   Purpose

   Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and DsbA (leaderless thiol:disulfide interchange protein DsbA from E. coli) fusions with optional C-terminal StrepII-tag

   Depositor

   Marko Hyvönen

   Type

   Empty backbone

   Use

   Tags

   8xHis-DsbA(SS) and Strep tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCas9CR4-VQR

    Plasmid

    #120224

    Purpose

    pCas9CR4 with VQR mutations for changing PAM site specificity to NGAN or NGNG from Kleinstiver et al. 2015

    Depositor

    Christopher Reisch

    Insert

    SpCas9-VQR
    

    Use

    Synthetic Biology

    Tags

    ssrA

    Expression

    Mutation

    Mutations D1135V/R1335V/T1337R

    Promoter

    pTet

    Available since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET mClover LIC cloning vector (H6-mClover)

    Plasmid

    #86931

    Purpose

    pET LIC cloning vector for Biotin-His6-tev-yORF-mClover

    Depositor

    Chris Jeans

    Type

    Empty backbone

    Use

    Tags

    6xHis, AviTag (biotin), TEV cleavage site, and mC…

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLS1/-11

    Plasmid

    #31492

    Depositor

    Kathleen Matthews

    Insert

    Lactose repressor with 11 amino acid C-terminal deletion (lacI E. coli variant)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 21, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pet28a+_Yjjg

    Plasmid

    #220228

    Purpose

    Bacterial expression of Yjjgin E. coli BL21_DE3 (pLys) or similar

    Depositor

    Bernd Nidetzky

    Insert

    Pyrimidine 5'-nucleotidase YjjG (yjjG E.coli)
    

    Use

    Tags

    Histag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pet15b _yein

    Plasmid

    #220226

    Purpose

    Bacterial expression of YeiN in E. coli BL21_DE3 (pLys) or similar

    Depositor

    Bernd Nidetzky

    Insert

    psug (psuG E.coli)
    

    Use

    Tags

    Histag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. FtsZ-MEEVF

    Plasmid

    #74126

    Purpose

    Expresses FtsZ fused to the MEEVF peptide tag via a flexible linker

    Depositor

    Lynne Regan

    Insert

    FtsZ-MEEVF
    

    Use

    Tags

    C-terminal linker sequence with the MEEVF tag (se…

    Expression

    Bacterial

    Mutation

    Promoter

    T5-lac

    Available since

    Aug. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pF2-DTA

    Plasmid

    #42521

    Depositor

    Terry Magnuson

    Article

    Jang et al PLoS One. 2013;8(2):e57075. doi: 10.1371

    Insert

    EF1a-DTA
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTC-0X1L

    Plasmid

    #20315

    Depositor

    Dirk Schnappinger

    Article

    Klotzsche et al Nucleic Acids Res. 2009 Jan 27. ():.

    Insert

    Pmyc1tetO-lacZ (lacZ E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 4, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET15b His6-ecDHFR (WT)

    Plasmid

    #73188

    Purpose

    bacterial express ecDHFR protein for washing out TMP ligand for pBMN DHFR(DD)-YFP (Plasmid #29325) and pBMN YFP-DHFR(DD) (Plasmid #29326)

    Depositor

    Thomas Wandless

    Insert

    folA dihydrofolate reductase (folA E. coli)
    

    Use

    Tags

    His 6X

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    Feb. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pOKD4

    Plasmid

    #17214

    Depositor

    Hao Wu

    Article

    Dzivenu et al Protein Expr Purif. 2004 Nov . 38(1):1-8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pOKD5

    Plasmid

    #17215

    Depositor

    Hao Wu

    Article

    Dzivenu et al Protein Expr Purif. 2004 Nov . 38(1):1-8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pEB354

    Plasmid

    #22069

    Depositor

    Emmanuelle Bouveret

    Article

    Gully et al Proteomics. 2006 Jan . 6(1):282-93.

    Type

    Empty backbone

    Use

    Tags

    T25

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET MBP mClover LIC cloning vector (MBP-mClover)

    Plasmid

    #86930

    Purpose

    pET LIC cloning vector for Biotin-His6-MBP-N10-tev-yORF-mClover

    Depositor

    Chris Jeans

    Type

    Empty backbone

    Use

    Tags

    6xHis, AviTag (biotin), MBP, TEV cleavage site, a…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHL 636

    Plasmid

    #53015

    Purpose

    AmpR + PLtetO-1::RBS (st7) fimE::T1 terminator + PLlacO-1::RBS (st7) fimB::Asp terminator + ColE1

    Depositor

    Han Lim

    Inserts

    fimE
    fimB
    

    Use

    Synthetic Biology

    Tags

    Asp terminator, RBS(st7), and T1 terminator

    Expression

    Bacterial

    Mutation

    Promoter

    pLlacO-1 and pLtetO-1

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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24. pLC13

    Plasmid

    #78494

    Purpose

    Expression of Mu-gam under the pBAD promoter

    Depositor

    David Bikard

    Article

    Cui et al Nucleic Acids Res. 2016 Apr 8. pii: gkw2

    Insert

    Mu-gam (Mup10 )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET His6 High pI protein 1 TEV LIC cloning vector (2-HpI-1)

    Plasmid

    #55210

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    6xHis, High pI protein 1, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET MBP tdTomato LIC cloning vector (MBP-tdTomato)

    Plasmid

    #86927

    Purpose

    pET LIC cloning vector for Biotin-His6-MBP-N10-tev-yORF-tdTomato

    Depositor

    Chris Jeans

    Type

    Empty backbone

    Use

    Tags

    6xHis, AviTag (biotin), MBP, TEV cleavage site, a…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET His6 High pI protein 85 TEV LIC cloning vector (2-HpI-85)

    Plasmid

    #55213

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    6xHis, High pI protein 85, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET His6 High pI protein 68 TEV LIC cloning vector (2-HpI-68)

    Plasmid

    #55211

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    6xHis, High pI protein 68, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pVP16-tev-PRT1

    Plasmid

    #83405

    Purpose

    bacterial expression vector pVP16 containing Arabidopsis thaliana PROTEOLYSIS 1 (PRT1) N-end rule E3 ubiquitin ligase to be used in N-end rule in vitro ubiquitination studies

    Depositor

    Nico Dissmeyer

    Insert

    PROTEOLYSIS1 (PRT1) (PRT1 E. coli, Mustard Weed)
    

    Use

    Tags

    His8, MBP, and tev cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pEB602

    Plasmid

    #22071

    Depositor

    Emmanuelle Bouveret

    Article

    Gully et al Proteomics. 2006 Jan . 6(1):282-93.

    Type

    Empty backbone

    Use

    Tags

    CBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge