We narrowed to 13,719 results for: RING

1. Integrin-β6 I domain I270C

   Plasmid

   #159639

   Purpose

   Contains integrin beta 6 residues 108–352 with I270C mutation

   Depositor

   Timothy Springer

   Article

   Dong et al Nature. 2017 Feb 2;542(7639):55-59. doi:

   Insert

   Integrin beta 6 I domain I270C
   

   Use

   Tags

   6x His

   Expression

   Mammalian

   Mutation

   I270C

   Promoter

   Available since

   Jan. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAct-Cas9

   Plasmid

   #62209

   Purpose

   Expresses Cas9 under control of act5C promoter and SV40 3'UTR. For ubiquitous genome engineering in Drosophila melanogaster.

   Depositor

   Simon Bullock

   Article

   Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

   Insert

   cas9
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   act5C

   Available since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. gRNA_AAVS1-T1

   Plasmid

   #41817

   Purpose

   Expresses a guide RNA (gRNA) to target human AAVS1 (T1 target sequence) for genome engineering

   Depositor

   George Church

   Article

   Mali et al Science. 2013 Feb 15;339(6121):823-6. do

   Insert

   gRNA_AAVS1-T1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Integrin-β6 Headpiece I270C

   Plasmid

   #159638

   Purpose

   Contains integrin beta 6 residues 1–474 with I270C mutation

   Depositor

   Timothy Springer

   Article

   Dong et al Nat Struct Mol Biol. 2014 Dec;21(12):109

   Insert

   Integrin beta 6 headpiece I270C
   

   Use

   Tags

   6x His

   Expression

   Mammalian

   Mutation

   I270C

   Promoter

   Available since

   Jan. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Integrin-αv Headpiece M400GC

   Plasmid

   #159637

   Purpose

   Contains integrin alpha v residues 1–594 with the M400C mutation and Gly inserted prior to residue 400

   Depositor

   Timothy Springer

   Article

   Dong et al Nat Struct Mol Biol. 2014 Dec;21(12):109

   Insert

   alpha v headpiece M400GC
   

   Use

   Tags

   6x His and Strep II

   Expression

   Mammalian

   Mutation

   M400C

   Promoter

   Available since

   Dec. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pnos-Cas9-nos

   Plasmid

   #62208

   Purpose

   Expresses Cas9 under control of nanos promoter and 3'UTR. For germ line restricted genome engineering in Drosophila melanogaster.

   Depositor

   Simon Bullock

   Article

   Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

   Insert

   cas9
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   nanos

   Available since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAJE-Ma pylRS nitroY/haloY-F5

   Plasmid

   #225684

   Purpose

   Expresses Methanomethylophilus alvus pyrrolysine tRNA synthetase engineered for 3-nitroY and 3-haloY under GlnS promoter. Used as a control during Ma Pyl tRNA-synthetase validation.

   Depositor

   Ryan Mehl

   Inserts

   M. alvus PylRS 3-NY/HaloY F5
   M. alvus Pyl-tRNA(6)
   

   Use

   Methanomethylophilus alvus

   Tags

   Expression

   Mutation

   CTG125CTT, N166A, V168S, A223C, W239R

   Promoter

   GlnS and Lpp

   Available since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSECRETS-A

   Plasmid

   #196986

   Purpose

   For SECRETS protocol to screen for gRNA activity and specificity: bacterial expression Cas9 in the presence of anhydrotetracycline (aTc).

   Depositor

   Eric Josephs

   Article

   Herring-Nicholas et al Commun Biol. 2024 Jan 12;7(1):86. doi: 1

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pltetO1

   Available since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. gRNA_GFP-T1

   Plasmid

   #41819

   Purpose

   Expresses a guide RNA (gRNA) to target GFP (T1 target sequence) for genome engineering

   Depositor

   George Church

   Article

   Mali et al Science. 2013 Feb 15;339(6121):823-6. do

   Insert

   gRNA_GFP-T1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFA6a-3HA-TRP1

    Plasmid

    #41599

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    TRP1 (TRP1 Budding Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    3xHA and yeast ADH1 terminator

    Expression

    Mutation

    Promoter

    Available since

    May 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLBH559_Tet-HisCas14a1Locus

    Plasmid

    #112502

    Purpose

    Expresses 10xHis-Cas14a1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter

    Depositor

    Jennifer Doudna

    Article

    Harrington et al Science. 2018 Oct 18. pii: science.aav42

    Insert

    Cas14a1
    

    Use

    CRISPR

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Tet

    Available since

    Oct. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. FnR9-10

    Plasmid

    #182567

    Purpose

    Human fibronectin fragment, type-III repeats 9-10, R1379A mutant

    Depositor

    Timothy Springer

    Article

    Su et al J Biol Chem. 2022 Sep;298(9):102323. doi

    Insert

    Human fibronectin fragment, type-III repeats 9-10
    

    Use

    Tags

    His and TEV cleavage site after His tag

    Expression

    Bacterial

    Mutation

    R1379A

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. gRNA_GFP-T2

    Plasmid

    #41820

    Purpose

    Expresses a guide RNA (gRNA) to target GFP (T2 target sequence) for genome engineering

    Depositor

    George Church

    Article

    Mali et al Science. 2013 Feb 15;339(6121):823-6. do

    Insert

    gRNA_GFP-T2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFA6a-GFP(S65T)-TRP1

    Plasmid

    #41597

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    TRP1 (TRP1 Budding Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    GFP (S65T) and yeast ADH1 terminator

    Expression

    Mutation

    Promoter

    Available since

    Jan. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFA6a-13Myc-TRP1

    Plasmid

    #41601

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    TRP1 (TRP1 Budding Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    13Myc and yeast ADH1 terminator

    Expression

    Mutation

    Promoter

    Available since

    Dec. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGEX-parkin WT

    Plasmid

    #45969

    Depositor

    Kalle Gehring

    Article

    Trempe et al Science. 2013 May 9.

    Insert

    Parkin (Prkn Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Codon usage optimized for E.coli expression

    Promoter

    tac

    Available since

    Sept. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLBH545_Tet-Cas14a1_Locus

    Plasmid

    #112501

    Purpose

    Expresses Cas14a1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter

    Depositor

    Jennifer Doudna

    Article

    Harrington et al Science. 2018 Oct 18. pii: science.aav42

    Insert

    Cas14a1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tet

    Available since

    Oct. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. ELK1 pcDNA3.1/HisC

    Plasmid

    #121093

    Purpose

    Expression of full length ELK1 in mammalian cells

    Depositor

    Paul Herring

    Article

    Zhou et al Mol Cell Biol. 2005 Nov . 25(22):9874-85

    Insert

    ELK1 (Elk1 Mouse)
    

    Use

    Tags

    6XHis-Omni-xpress

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTJV1ScKm-rpoB

    Plasmid

    #166980

    Purpose

    Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; npt2 for kanamycin resistance

    Depositor

    Christopher Reisch

    Article

    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

    Insert

    NeoR/KanR
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. Human p150,95

    Plasmid

    #8633

    Depositor

    Timothy Springer

    Article

    Corbi et al EMBO J 1987 Dec 20;6(13):4023-8.

    Insert

    Human p150,95 (ITGAX Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge