We narrowed to 1,234 results for: cfp

1. pQE60-mPU.1-ETS

   Plasmid

   #85733

   Purpose

   Expresses DNA-binding (ETS) domain of murine PU.1, residues 167-272, in M15 cells already pre-transformed with 2nd plasmid, pREP-4

   Depositor

   Gregory Poon

   Article

   Poon et al J Biol Chem. 2012 May 25;287(22):18297-3

   Insert

   ETS domain of transcription factor PU.1 (Spi1 Mouse)
   

   Tags

   6xHis tag

   Expression

   Bacterial

   Available Since

   April 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. mCit-Snap25b D179K A199R

   Plasmid

   #53259

   Purpose

   SNAP25B double mutant insensitive to both Botulinum toxin C1 & E; N-terminal mCitrine tag connected by 4 amino acid linker. For mammalian cells, used with mCer-4aa-VAMP2 to measure FRET & dispersion.

   Depositor

   Robert Zucker

   Article

   Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

   Insert

   SNAP-25B (Snap25 Rat)
   

   Tags

   4aa-linker and mCitrine

   Expression

   Mammalian

   Mutation

   D179K and A199R

   Promoter

   CMV

   Available Since

   April 21, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pmTol-InsUAS-6MTnlstdTomato2A-Dusp6DM-CC

   Plasmid

   #67687

   Purpose

   UAS-driven 6xMyc nuclear localized tdTomato-2A-activated Dusp6 in minTol backbone; also with cry:cfp cassette

   Depositor

   Nathan Lawson

   Article

   Shin et al Development. 2016 Oct 15;143(20):3796-38

   Insert

   dusp6 (dusp6 Zebrafish)
   

   Tags

   6xmyc-NLS-tdTomato-2A

   Mutation

   S159A, S196A

   Available Since

   Oct. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAFNF-Venus-Actb

   Plasmid

   #169788

   Purpose

   An actin FRET sensor. Must be expressed with ECFP-actin for FRET.

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Actb (Actb Mouse)
   

   Tags

   Venus

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. OA-1045B

   Plasmid

   #125004

   Purpose

   Expresses gRNAs targeting hid, eve, and hedgehog

   Depositor

   Omar Akbari

   Article

   Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

   Insert

   eve gRNA (eve Fly)
   

   Expression

   Insect

   Promoter

   U6:3

   Available Since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. OA-1045E

   Plasmid

   #125007

   Purpose

   Expresses tRNA-flanked gRNAs targeting hid, eve, and hedgehog

   Depositor

   Omar Akbari

   Article

   Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

   Insert

   eve gRNA (eve Fly)
   

   Expression

   Insect

   Promoter

   U6:3

   Available Since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. OA-1045A

   Plasmid

   #125003

   Purpose

   Expresses gRNAs targeting hid and eve

   Depositor

   Omar Akbari

   Article

   Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

   Insert

   eve gRNA (eve Fly)
   

   Expression

   Insect

   Promoter

   U6:3

   Available Since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSLQ10878

   Plasmid

   #183964

   Purpose

   CAG-hyperCas12a(nuclease active)-HA-P2A-mCherry-T2A-HygroR

   Depositor

   Stanley Qi

   Article

   Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

   Insert

   HyperdCas12a
   

   Use

   CRISPR

   Tags

   HA, Hygromycin Resistance, and mCherry

   Expression

   Mammalian

   Mutation

   D156R, E292R, D235R, D350R

   Promoter

   CAG

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-Lyn-ICUE3

   Plasmid

   #61623

   Purpose

   Plasma membrane-targeted FRET biosensor for monitoring cAMP

   Depositor

   Jin Zhang

   Article

   DiPilato et al Mol Biosyst. 2009 Aug;5(8):832-7. doi: 1

   Insert

   Lyn-ICUE3 (RAPGEF3 Human)
   

   Tags

   ECFP, N-terminal targeting sequence from Lyn kina…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSLQ10873

    Plasmid

    #183961

    Purpose

    U6-SKSKSO(crRNA array)-CAG-hyperdCas12a-HA-miniVPR

    Depositor

    Stanley Qi

    Article

    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

    Inserts

    HyperdCas12a
    poly-crRNA array targeting Sox2-Klf4-Oct4
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D832A, D156R, E292R, D235R, D350R

    Promoter

    CAG and U6

    Available Since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. FAT FAK biosensor

    Plasmid

    #78303

    Purpose

    FRET biosensor. Visualization of FAK activity at focal adhesions in live cells

    Depositor

    Yingxiao Wang

    Article

    Seong et al Proc Natl Acad Sci U S A. 2013 Nov 26;11

    Insert

    FAT FAK biosensor
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 13, 2016

    Availability

    Academic Institutions and Nonprofits only

12. alpha catenin conformation sensor

    Plasmid

    #71709

    Purpose

    This alpha catenin biosensor undergoes a change in FRET upon mechanical perturbation of the alpha catenin conformation.

    Depositor

    Deborah Leckband

    Article

    Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi

    Insert

    alpha-catenin FRET Sensor
    

    Tags

    ECFP and YPET

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-FKBP-ICUE3-NLS

    Plasmid

    #138255

    Purpose

    FRET-based cAMP indicator fused to FKBP (rapamycin-binding domain); nuclear targeted. Used in conjuction with pcDNA3.1(+)-AKAP95-FRB to monitor cAMP dynamics within AKAP95 nanodomain.

    Depositor

    Jin Zhang

    Article

    Clister et al Cell Chem Biol. 2019 Jun 20;26(6):885-89

    Insert

    FKBP-ICUE3-NLS
    

    Tags

    FKBP, Nuclear localization signal (NLS), cpVenus[…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV CytoBAS

    Plasmid

    #62860

    Purpose

    Fluorescent bile acid sensor (BAS) that allows spatiotemporal monitoring of bile acid transport in single living cells using FRET

    Depositor

    Stan van de Graaf

    Article

    van der Velden et al Hepatology. 2013 Feb;57(2):740-52. doi:

    Insert

    CytoBAS
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV NucleoBAS

    Plasmid

    #62861

    Purpose

    Fluorescent bile acid sensor (BAS) targeted to nucleus that allows spatiotemporal monitoring of bile acid transport in single living cells using FRET

    Depositor

    Stan van de Graaf

    Article

    van der Velden et al Hepatology. 2013 Feb;57(2):740-52. doi:

    Insert

    NucleoBAS
    

    Tags

    SV40 NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCS-CG-WT-Cer-VWF

    Plasmid

    #124827

    Purpose

    Expresses multimeric human VWF-ECFP fusion protein. The cyan fluorescence protein coding region is inserted between the VWF-D'D3 and A1 domains.

    Depositor

    Sriram Neelamegham

    Article

    Zhang et al Blood Adv. 2019 Apr 9;3(7):957-968. doi:

    Insert

    VWF with cyan fluorescent protein fusion between D'D3 and A1 domains (VWF Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBullet-pm-c

    Plasmid

    #53078

    Purpose

    destination vector with PIP2a-ECFP (plasma membrane marker) for tagged fluorescent protein (C-ter-EYFP) of plant gene

    Depositor

    Joshua Heazlewood

    Article

    Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. HOXB1 (human) HIS-tag pET

    Plasmid

    #8520

    Depositor

    Corey Largman

    Article

    Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

    Insert

    HOXB1 (HOXB1 Human)
    

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    PCR from cDNA clone

    Available Since

    July 6, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pBullet-pm-n

    Plasmid

    #53077

    Purpose

    destination vector with PIP2a-ECFP (plasma membrane marker) for tagged fluorescent protein (N-ter-EYFP) of plant gene

    Depositor

    Joshua Heazlewood

    Article

    Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. H3K9me3 biosensor

    Plasmid

    #120802

    Purpose

    FRET biosensor. To monitor histone H3 lysine 9 tri-methylation in mammalian cells by FRET

    Depositor

    Yingxiao Wang

    Article

    Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

    Insert

    Truncated YPet-HP1-EV linker-ECFP-mouse histone H3
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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