We narrowed to 4,396 results for: erf

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pGEX-4T-1-p19-T7-PLK1

Plasmid

#46312

Depositor

Judy Lieberman

Article

Huang et al Nat Biotechnol. 2013 Apr;31(4):350-6. do

Inserts

p19
PLK1 DNA-1
linker
PLK1 DNA-2

Use

Pro-sirna

Tags

6XHis and GST

Expression

Bacterial

Available Since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only
Pr-deGFP-MGapt

Plasmid

#67734

Purpose

OR2-OR1-Pr promoter expressing deGFP-MG aptamer fusion

Depositor

Richard Murray

Article

Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

Insert

UTR1-deGFP-MGapt-T500

Use

Synthetic Biology

Expression

Bacterial

Promoter

OR2-OR1-Pr

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pHW-CIBN-MP-HRW-CRY2-VHH(GFP)

Plasmid

#126024

Purpose

express RFP-tagged LARIAT for optogenetic clustering

Depositor

Eurico Morais-de-Sá

Article

Osswald et al Biomolecules. 2019 Feb 12;9(2). pii: bio

Insert

CIBN-MP; CRY2 PHR-VHH(GFP)

Tags

RFP

Expression

Insect

Mutation

WT

Promoter

Hsp70

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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GTT-g

Plasmid

#81109

Purpose

Gene Tagging vector TRP/GFP - Plasmid for superfolder GFP (sfGFP) labeling of endogenous proteins with a TRP selection marker, originating from pSIVt (ID:81092)

Depositor

Serge Pelet

Article

Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

Insert

sfGFP

Use

Cre/Lox

Tags

sfGFP

Expression

Yeast

Available Since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pSLC-239

Plasmid

#73193

Purpose

Template plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (yhaV) under the control of rhamnose induceable promoter (PrhaB) for negative selection.

Depositor

Swaine Chen

Article

Khetrapal et al Nucleic Acids Res. 2015 Jul 27;43(13):e8

Insert

PrhaB-yhaV (yhaV )

Expression

Bacterial

Promoter

PrhaB

Available Since

July 15, 2016

Availability

Academic Institutions and Nonprofits only

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mNG-mTq2

Plasmid

#124217

Purpose

Cytoplasmic mNeongreen mTurquoise2 tandem, ~60 % energy transfer. pNM043 - pSAV057-mNG-mTq2

Depositor

Tanneke den Blaauwen

Article

Meiresonne et al Mol Microbiol. 2019 Apr;111(4):1025-1038

Insert

mNG-mTq2

Expression

Bacterial

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pGL3-U6-sgRNA-PGK-puromycin-AflII

Plasmid

#106404

Purpose

Empty guide RNA cloning vector with an AflII site added from Addgene 41824

Depositor

Jason Gertz

Article

Carleton et al Cell Syst. 2017 Oct 25;5(4):333-344.e5.

Type

Empty backbone

Expression

Mammalian

Promoter

U6

Available Since

April 25, 2018

Availability

Academic Institutions and Nonprofits only

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pEN_TGmiR_Luc-A

Plasmid

#25765

Purpose

Entry vector withTRE promoter driving control Luciferase miR30-based shRNA with GFP coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

Luciferase miR-shRNA

Use

Entry vector

Tags

GFP

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pCol-TGM-luc.1309

Plasmid

#32720

Depositor

Scott Lowe

Article

Premsrirut et al Cell. 2011 Apr 1;145(1):145-58.

Insert

luciferase

Use

Mouse Targeting and RNAi

Promoter

TRE and Tre

Available Since

March 2, 2012

Availability

Academic Institutions and Nonprofits only
pAct5C-Hh

Plasmid

#37382

Depositor

Norbert Perrimon

Article

Nybakken et al Nat Genet. 2005 Dec;37(12):1323-32. Epub

Insert

hedgehog (hh Fly)

Expression

Insect

Promoter

actin5C

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
CROP_C9_Puro_VEGFB

Plasmid

#183328

Purpose

All-in-One CRISPRko system with a guide RNA that targets VEGFB gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

VEGFB

Use

Lentiviral

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_VEGFA

Plasmid

#183327

Purpose

All-in-One CRISPRko system with a guide RNA that targets VEGFA gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

VEGFA

Use

Lentiviral

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pPRIME-TET-GIN-recipient

Plasmid

#11656

Purpose

3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with a tetracycline-responsive promoter (TET) controlling GFP-IRES-Neo expression

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Type

Empty backbone

Use

Lentiviral

Tags

GFP, IRES, and Neo

Expression

Mammalian

Available Since

March 1, 2007

Availability

Academic Institutions and Nonprofits only

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pTRCHisB-TRF2∆B

Plasmid

#53208

Purpose

Expressed N-terminally Hexahistine tagged TRF2 Delta Basic protein

Depositor

Jack Griffith

Article

Bower et al Biochemistry. 2014 Sep 2;53(34):5485-95.

Insert

TRF2 Delta Basic (TERF2 Human)

Tags

6x His

Expression

Bacterial

Mutation

contains amino acids 47-500

Promoter

Trc

Available Since

June 19, 2014

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 PDGFRB

Plasmid

#67130

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

PDGFRB (PDGFRB Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pALPS_3'LTR GFP@gag start SFFV-

Plasmid

#101337

Purpose

Repaired U3 allows LTR-based transcription by the provirus with GFP as a marker for expression. WPRE was deleted.

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

GFP

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_GART

Plasmid

#183296

Purpose

All-in-One CRISPRko system with a guide RNA that targets GART gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

GART

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pBXMCS-2 Pconstitutive-sgRNA(Sth3)_gcrA1

Plasmid

#133340

Purpose

for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets gcrA gene's promoter on the non-template strand in Caulobacter crescentus

Depositor

Michael Laub

Article

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

sgRNA_gcrA

Use

CRISPR

Promoter

constitutive

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pHW-CIBN-MP-HFW-CRY2-VHH(GFP)

Plasmid

#126027

Purpose

express Flag-tagged LARIAT for optogenetic clustering

Depositor

Eurico Morais-de-Sá

Article

Osswald et al Biomolecules. 2019 Feb 12;9(2). pii: bio

Insert

CIBN-MP; CRY2 PHR-VHH(GFP)

Tags

Flag

Expression

Insect

Mutation

WT

Promoter

Hsp70

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pEN_TRmiRc2

Plasmid

#25750

Purpose

Entry vector for cloning miR30-based shRNA driven by TRE promoter with dsRed2 coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Entry vector

Tags

dsRed2

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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