We narrowed to 17,203 results for: puro

1. pLentipuro3/TO/V5-GW/EGFP-Firefly Luciferase

   Plasmid

   #119816

   Purpose

   3rd generation lentiviral plasmid for expression of EGFP Firefly Luciferase fusion protein in mammalian cells

   Depositor

   Ethan Abel

   Article

   Lorenzatti Hiles et al Nat Protoc. 2019 Mar;14(3):738-755. doi:

   Inserts

   Luciferase
   Puromycin
   

   Use

   Lentiviral and Luciferase

   Tags

   EGFP and V5

   Expression

   Mammalian

   Mutation

   Stop codon removed in the frame of the V5 frame v…

   Promoter

   CMV/TO and Human Phosphoglycerate kinase

   Available since

   Jan. 2, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pLenti CMV MCL1 Puro

   Plasmid

   #140746

   Purpose

   Expresses MCL1 in mammalian cells

   Depositor

   Scott Dixon

   Article

   Inde et al Cell Rep. 2020 Jul 7;32(1):107845. doi:

   Insert

   MCL1 (MCL1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLD-puro-MTS-EGFP

   Plasmid

   #190270

   Purpose

   Lentiviral conditional expression of mitochondrial-targeted eGFP in mammalian cells

   Depositor

   Christian Münch

   Article

   Michaelis et al Bio Protoc. 2022 Dec 20;12(24):e4578. do

   Insert

   MTS-EGFP
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Tight TRE promoter

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBABE 3xHA-MKK6-EE puro

   Plasmid

   #216791

   Purpose

   Mammalian expression of constitutively active human MKK6

   Depositor

   Kevin Janes

   Insert

   MKK6 (MAP2K6 Human)
   

   Use

   Retroviral

   Tags

   3x-HA

   Expression

   Mammalian

   Mutation

   S207E and T211E, constitutively active

   Promoter

   LTR

   Available since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. FUW-Luc-mCherry-puro

   Plasmid

   #183685

   Purpose

   Lentiviral vector expressing luciferase and mCherry.

   Depositor

   Andrew Kung

   Article

   Kimbrel et al Mol Imaging. 2009 May-Jun;8(3):140-7.

   Insert

   Luc-E2A-mCherry-T2A-PuroR
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Human Ubiquitin C (UbC) promoter

   Available since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLVX-Puro-TDP-43-A315T

   Plasmid

   #133755

   Purpose

   Lentiviral expression of human TDP-43 A315T disease mutant in mammalian cells.

   Depositor

   Shawn Ferguson

   Article

   Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

   Insert

   TARDBP (TARDBP Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Changed alanine 315 to threonine

   Promoter

   CMV

   Available since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLVX-Puro-TDP-43-Q331K

   Plasmid

   #133761

   Purpose

   Lentiviral expression of human TDP-43 Q331K disease mutant in mammalian cells.

   Depositor

   Shawn Ferguson

   Article

   Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Changed glutamine 331 to lysine.

   Promoter

   CMV

   Available since

   Dec. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pPBT/EFS-Cas9-PuroTk

   Plasmid

   #170542

   Purpose

   Expresses spCas9 with a P2A junction to the puromycin resistance gene directly fused to the thymidine kinase

   Depositor

   Jacob Giehm Mikkelsen

   Article

   Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

   Insert

   PuroTK
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLD-puro-Cc-TARDBP-WT_VA

   Plasmid

   #141327

   Purpose

   Lentiviral vector for expression of C-terminally VA-tagged human TARDBP-WT in mammalian cells

   Depositor

   Mohan Babu

   Article

   Pourhaghighi et al Cell Syst. 2020 Apr 22;10(4):333-350.e14

   Insert

   TARDBP-WT (TARDBP Human)
   

   Use

   Lentiviral

   Tags

   VA tag (Beacon-2XStrep-6XHis-2XTEV-3XFLAG)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. lenti-pegRNA-puro backbone

    Plasmid

    #214085

    Purpose

    Lentiviral backbone plasmid to insert pegRNAs of interest. The cloning site is BsmbI.

    Depositor

    Ting Zhou

    Article

    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6, EF1a

    Available since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHDR_DDX4-T2A-tdTomato-puroTK

    Plasmid

    #222519

    Purpose

    Homology directed repair template for knocking in tdTomato reporter to DDX4.

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2024.05.31.596483

    Insert

    tdTomato
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLKO.1 Puro shRNA Scramble

    Plasmid

    #162011

    Purpose

    Lentiviral negative control vector containing scramble shRNA

    Depositor

    David Bryant

    Article

    Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

    Insert

    scramble shRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBOB-EF1-FastFUCCI-Puro

    Plasmid

    #86849

    Purpose

    3rd generation lentiviral vector encoding FUCCI reporters for cell cycle analysis and puro for selection

    Depositor

    Duncan Jodrell, Kevin Brindle

    Article

    Koh et al J Cell Sci. 2016 Nov 25. pii: jcs.195164

    Inserts

    mKO2-hCDT1(30-120)
    mAG-hGEM(1-110)
    pac (puromycin N-acetyl-transferase)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1 and PGK

    Available since

    Nov. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLVX-TetOne-Puro-GFP

    Plasmid

    #171123

    Purpose

    Doxycycline inducible expression of GFP

    Depositor

    Jason Sheltzer

    Article

    Lukow et al Dev Cell. 2021 Sep 13;56(17):2427-2439.e

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSpCas9n(BB)-2A-Puro (PX462) V2.0

    Plasmid

    #62987

    Purpose

    Cas9n (D10A nickase mutant) from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Insert

    hSpCas9n-2A-Puro
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Cas9 D10A nickase mutant

    Promoter

    Available since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV-PuroR-T2A-eGFP

    Plasmid

    #234734

    Purpose

    Puromycin resistance/eGFP cassette in mammalian cells (transfection marker)

    Depositor

    David Liu

    Article

    Witte et al Science. 2025 May 15;388(6748):eadt5199.

    Insert

    PuroR-T2A-eGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. FASTHDR-Cterm-mClover3-Puromycin

    Plasmid

    #167205

    Purpose

    Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mClover3 and selection with Puromycin

    Depositor

    Oscar Perez

    Article

    Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    mClover3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLentiCMV Puro DEST p38KTRmCerulean3

    Plasmid

    #59155

    Purpose

    Lentiviral vector to express p38 KTR mCerulean3 under CMV promoter (With Puromycin Resistance)

    Depositor

    Markus Covert

    Article

    Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

    Insert

    p38 Kinase Translocation Reporter (MAPK14 Human, Mouse)
    

    Use

    Lentiviral

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ZsGreen1-cMYC/pLVX-Puromycin

    Plasmid

    #180278

    Purpose

    Plasmid encoding human c-MYC for reporter assays. Polycistronic puromycin selectable lentiviral vector (unmodified, from TAKARA BIO).

    Depositor

    Andre Catic

    Article

    Moree et al Cancers (Basel). 2022 Feb 4;14(3). pii:

    Insert

    ZsGreen1-cMYC fusion protein MYC (MYC Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMSCV-U6sgRNA(BbsI)-PGKpuro2ABFP

    Plasmid

    #102796

    Purpose

    Retrovirus for delivery of one sgRNA (empty, bbsI)

    Depositor

    Sarah Teichmann

    Article

    Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

    Insert

    U6 sgRNA
    

    Use

    CRISPR and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge