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Showing: 11981 - 12000 of 68611 results

  3. pMXS-GshF-blasticidin

    Plasmid
    #210313
    Purpose
    Expresses humanized Streptococcus thermophilus GshF in mammalian cells
    Depositor
    Kivanc Birsoy
    Publication
    Wang et al Nature. 2021 Nov;599(7883):136-140. doi:
    Insert
    Bifunctional glutamate-cysteine ligase/glutathione synthetase GshF (gshAB Streptococcus thermophilus)
    Use
    Retroviral
    Tags
    FLAG peptide
    Mutation
    Codon optimized for expression in human cells
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    210313-plasmid-map-sequence-id-418847

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  4. pCXLE-Sox2-17-T2A-Klf4-E2A-Myc

    Plasmid
    #210016
    Purpose
    episomal S*KM (expressing mouse super-Sox + Klf4 + Myc) for integration-free naïve reset
    Depositor
    Hans Schöler
    Publication
    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Sox2-17-t2a-Klf4-e2a-cMyc (Klf4, Sox2 Mouse)
    Expression
    Mammalian
    Mutation
    Sox2-17 is engineered highly cooperative chimeric…
    Promoter
    CAG
    Available since
    Dec. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    210016-plasmid-map-sequence-id-418270

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  5. piggyBac-rtTA (4th_Gen)-SNCA(A53T)-sfGFP-IRES-NGN2-puro (VK_1123)

    Plasmid
    #209080
    Purpose
    Ngn2 mediated cortical neuron induction, along with SNCA(A53T)-sfGFP over-expression
    Depositor
    Vikram Khurana
    Publication
    Lam et al Neuron. 2024 Sep 4;112(17):2886-2909.e16
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    SNCA (SNCA Human)
    NGN2 (Neurog2 Mouse)
    Tags
    sfGFP
    Expression
    Mammalian
    Mutation
    A53T
    Promoter
    TRE4G
    Available since
    Dec. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    209080-plasmid-map-sequence-id-416548

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  9. pS648∙CsR

    Plasmid
    #197860
    Purpose
    Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria
    Depositor
    Susanne Häußler
    Publication
    Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    gRNA scaffold - Gm resistance cassette - incP oriT
    Use
    CRISPR and Synthetic Biology
    Promoter
    PEM7
    Available since
    Oct. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    197860-plasmid-map-sequence-id-402537

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  10. KRAB-dSpCas9

    Plasmid
    #205248
    Purpose
    In vitro transcription plasmid for production of KOX1 KRAB-dSpCas9 mRNA for transcriptional repression (KRAB = Krüppel-associated box). Insert is derived from Addgene plasmid #85449.
    Depositor
    Rasmus Bak
    Publication
    Jensen et al Genome Res. 2021 Nov;31(11):2120-2130. d
    Insert
    KRAB-dSpCas9
    Use
    CRISPR
    Available since
    Sept. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    205248-plasmid-map-sequence-id-409108

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  12. TFORF3015

    Plasmid
    #144491
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Publication
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    PPARA (PPARA Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Aug. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    144491-plasmid-map-sequence-id-405320

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  13. TFORF3247

    Plasmid
    #144723
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Publication
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    LEF1 (LEF1 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144723-plasmid-map-sequence-id-404303

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  14. TFORF2908

    Plasmid
    #143663
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Publication
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NFIC (NFIC Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    143663-plasmid-map-sequence-id-400467

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  17. pKrox24(MapErk)Luc

    Plasmid
    #200112
    Purpose
    Luciferase reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity
    Depositor
    Pavel Krejčí
    Publication
    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MapErk sequence in minimal promoter
    Expression
    Mammalian
    Mutation
    highly modified sequence
    Promoter
    5 copies of designed MapErk sequence in minimal p…
    Available since
    June 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    200112-plasmid-map-sequence-id-399196

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Showing: 11981 - 12000 of 68611 results