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pcDNA3.1(+)-HA-Flag-natT1R2

Plasmid

#113944

Purpose

mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin

Depositor

Erik Procko

Article

Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

Insert

T1R2 (TAS1R2 Human)

Tags

FLAG and Signal/leader sequence from influenza he…

Expression

Mammalian

Mutation

residues 22-839

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pDule2-Mb haloTyrRS C6

Plasmid

#160378

Purpose

C6 HaloTyrosine tRNA synthatase and cognate amber suppressing tRNA derived from M. barkeri.

Depositor

Ryan Mehl

Article

Jang et al ACS Chem Biol. 2020 Feb 21;15(2):562-574

Insert

C6 HaloTyrosine tRNA synthatase

Use

Synthetic Biology

Expression

Bacterial and Mammalian

Mutation

L270S, Y271L, N311G, C313T

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pBabe B-Raf V600E

Plasmid

#17544

Depositor

Channing Der

Article

Hao et al Mol Cancer Ther. 2007 Aug . 6(8):2220-9.

Insert

B-Raf (BRAF Human)

Use

Retroviral

Expression

Mammalian

Mutation

V600E

Available Since

March 21, 2008

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG94-15

Plasmid

#228971

Purpose

For bacterial expression of anti-GFP nanobody LaG94-15, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaG94-15

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-C-STAT1-mGFP-P2A-Puro

Plasmid

#199345

Purpose

express murine STAT1 protein fused with mGFP

Depositor

Massimo Mallardo

Article

Caiazza et al Int J Mol Sci. 2022 Nov 17;23(22):14232.

Insert

signal transducer and activator of transcription 1 (Stat1 Mouse)

Use

Lentiviral

Tags

mGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-rtTA-zeo

Plasmid

#183233

Purpose

Lentiviral vector expressing the rtTa gene

Depositor

Jorge Ferrer

Article

Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

Insert

rtTA-T2A-Zeo

Use

Lentiviral

Promoter

UBC

Available Since

Oct. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-N2-IFT20

Plasmid

#218719

Purpose

Expresses C-terminally EGFP-tagged IFT20 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

Insert

IFT20 (IFT20 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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pDM002-sgRNA-lenti-ZeoR

Plasmid

#200060

Purpose

Lentiviral vector for expression of sgRNA with mCherry, zeocin resistance, BlpI + BstXI cloning sites

Depositor

Britt Glaunsinger

Article

McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

Insert

Bleomycin resistance, mCherry

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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pAS_FLAG-G1 PylRS

Plasmid

#154763

Purpose

plasmid with G1 PylRS for amber suppression in transient or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

G1 PylRS (MJ_RS07805 methanogenic archaeon mixed culture ISO4-G1)

Tags

FLAG

Expression

Mammalian

Promoter

EF1

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pRK5 c-Fyn

Plasmid

#16032

Depositor

Filippo Giancotti

Article

Mariotti et al J Cell Biol. 2001 Oct 29. 155(3):447-58.

Insert

c-Fyn (FYN Human)

Expression

Mammalian

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

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pLenti Dest BlastR R1-R2

Plasmid

#84574

Purpose

Gateway destination vector expressing Blasticidin resistance under a PGK promoter

Depositor

Ghassan Mouneimne

Article

Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

Type

Empty backbone

Use

Lentiviral; Gateway

Expression

Mammalian

Promoter

PGK

Available Since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

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SGCD-His-bio

Plasmid

#52333

Purpose

Expresses full-length Delta-sarcoglycan ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

SGCD (SGCD Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pRSF-G1(5CNW)RS

Plasmid

#222872

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of 5-Cyano-L-Tryptophan (5-CN-Trp) into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Article

Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

Insert

tRNA synthetase

Expression

Bacterial

Promoter

T7

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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Brm WT-Flag pShuttle

Plasmid

#122105

Purpose

Expression of full length Brm in mammalian cells

Depositor

Paul Herring

Article

Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

Insert

BRM (SMARCA2 Human)

Tags

Flag

Expression

Mammalian

Available Since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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pMXs-IP spGFP-ERGIC53

Plasmid

#38270

Depositor

Noboru Mizushima

Article

Itakura et al Autophagy. 2010 Aug;6(6):764-76.

Insert

ERGIC-53 (LMAN1 Human)

Use

Retroviral

Tags

EGFP with Calreticulin signal peptide at N-termin…

Expression

Mammalian

Mutation

deleted signal peptide (1-30 amino acids)

Available Since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only

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myc-KIF5C

Plasmid

#127618

Purpose

N-terminal myc-tagged mouse KIF5C

Depositor

Josef Kittler

Article

Twelvetrees et al Neuron. 2010 Jan 14;65(1):53-65. doi: 10

Insert

KIF5C (Kif5c Mouse)

Tags

myc

Expression

Mammalian

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pHalo-MyosinIIB-C25

Plasmid

#128574

Purpose

Studies of cellular contractility.

Depositor

Dylan Burnette

Article

Fenix et al Elife. 2018 Dec 12;7. pii: 42144. doi: 1

Insert

Myosin IIB (MYH10 Human)

Tags

Halo

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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Lifeact-7-iRFP670

Plasmid

#103032

Purpose

Expression of iRFP670-tagged Lifeact with G418 selection in mammalian cells

Depositor

Ghassan Mouneimne

Article

Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

Insert

Lifeact

Tags

iRFP670

Expression

Mammalian

Promoter

CMV

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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FLAG-p54

Plasmid

#35379

Depositor

Benjamin Blencowe

Article

Rosonina et al Mol Cell Biol. 2005 Aug;25(15):6734-46.

Insert

p54 (NONO Human)

Tags

FLAG

Expression

Mammalian

Mutation

A464V

Promoter

CMV

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-Hs4E-BP1

Plasmid

#79438

Purpose

Expresses lambdaN-HA-tagged human 4E-BP1 in Mammalian cells

Depositor

Elisa Izaurralde

Article

Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

Insert

Hs4E-BP1 (EIF4EBP1 Human)

Tags

lambdaN-HA

Expression

Mammalian

Promoter

CMV

Available Since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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