165,886 results

1. CRF1R-GFP

   Plasmid

   #208912

   Purpose

   encodes the human corticotropin releasing factor 1 receptors, tagged with YFP

   Depositor

   Christian Gruber

   Article

   Fahradpour et al Front Pharmacol. 2017 Sep 25;8:616. doi:

   Insert

   CRHR1 (CRHR1 Human)
   

   Tags

   EYFP

   Expression

   Mammalian

   Available Since

   Oct. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHYRSF53

   Plasmid

   #64696

   Purpose

   His-Smt3-NpuDnaEN102-CBD

   Depositor

   Hideo Iwai

   Article

   Guerrero et al Protein Expr Purif. 2015 Aug 20. pii: S1

   Type

   Empty backbone

   Tags

   CBD, HisTag, and NpuDnaE-N102

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. proinsulin-EYFP

   Plasmid

   #218952

   Purpose

   Expresses proinsulin fused to EYFP in bacteria

   Depositor

   Benjamin Glick

   Insert

   proinsulin-EYFP
   

   Tags

   proinsulin

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. U6-5' MS2-CircRNA

   Plasmid

   #213750

   Purpose

   For circular RNA-mediated prime editor using U6-5' MS2-CircRNA in HEK293T cells

   Depositor

   Caixia Gao

   Article

   Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

   Insert

   5' ribozyme, 5' ligation sequences, 5' MS2, 3' ligation sequences, 3' ribozyme
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR223-LCK

   Plasmid

   #23890

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   LCK (LCK Human)
   

   Use

   Gateway donor vector

   Mutation

   V93I

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-cofilin-S3D

   Plasmid

   #78297

   Purpose

   Mammalian expression of mouse cofilin S3D fused to EGFP

   Depositor

   Michael Frotscher

   Article

   Chai et al Development. 2016 Mar 15;143(6):1029-40.

   Insert

   cofilin 1 (Cfl1 Mouse)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S3D

   Promoter

   CAG

   Available Since

   May 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKA-142

   Plasmid

   #79835

   Purpose

   Expresses PpsR2-mVenus-CAAX under CMVd1 promoter

   Depositor

   Vladislav Verkhusha

   Article

   Kaberniuk et al Nat Methods. 2016 May 9. doi: 10.1038/nm

   Insert

   PpsR2-mVenus-CAAX
   

   Expression

   Mammalian

   Mutation

   RpPpsR2 cysteine 439 changed to serine

   Promoter

   CMVd1

   Available Since

   Aug. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAcBac1-BacMam-4x-PyOtR

   Plasmid

   #217364

   Purpose

   Expresses four copies pyrrolysyl tRNA variant "PyOtR" with U6 promoter; can be packaged in bacmid

   Depositor

   Abhishek Chatterjee

   Article

   Jewel et al Angew Chem Int Ed Engl. 2024 Jan 26:e202

   Insert

   4 copies of pyrrolysyl-tRNA mutant PyOtR
   

   Use

   Baculoviral

   Expression

   Insect and Mammalian

   Promoter

   U6

   Available Since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR221-SLC7A9_STOP

   Plasmid

   #161411

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC7A9 (SLC7A9 Human)
   

   Expression

   Mammalian

   Available Since

   Sept. 15, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pDONR221-SLC7A6_STOP

    Plasmid

    #161375

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC7A6 (SLC7A6 Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 15, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pL7Ae-myc-T2A-tagRFP

    Plasmid

    #140263

    Purpose

    Expresses L7Ae in mammalian cells

    Depositor

    Hirohide Saito

    Article

    Ono et al ACS Synth Biol. 2020 Jan 17;9(1):169-174

    Insert

    L7Ae
    

    Use

    Synthetic Biology

    Tags

    myc-T2A-tagRFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcS-mCherry-C1C2

    Plasmid

    #178425

    Purpose

    Fluorescence reporter fused to EV membrane binding domain for EV imaging and tracking

    Depositor

    Masako Harada

    Article

    Komuro et al Nanotheranostics. 2021 Apr 15;5(4):378-3

    Insert

    mCherry
    

    Expression

    Mammalian

    Available Since

    July 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. MG-155

    Plasmid

    #26529

    Depositor

    David Baltimore

    Article

    O'Connell et al J Exp Med. 2008 Mar 17. 205(3):585-94.

    Insert

    miR-155 (MIR155 Human)
    

    Use

    Retroviral

    Tags

    eGFP

    Expression

    Mammalian

    Available Since

    Oct. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

14. ANO10 (TMEM16KA-c001)

    Plasmid

    #110267

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    TMEM16K (ANO10 Human)
    

    Tags

    TEV-His10-Flag

    Expression

    Insect

    Promoter

    polyhedrin promoter

    Available Since

    April 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. NMBR-Tango

    Plasmid

    #66445

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    NMBR (NMBR Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

16. U6-3' MS2-CircRNA

    Plasmid

    #213751

    Purpose

    For circular RNA-mediated prime editor using U6-3' MS2-CircRNA in HEK293T cells

    Depositor

    Caixia Gao

    Article

    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Insert

    5' ribozyme, 5' ligation sequences, 3' MS2, 3' ligation sequences, 3' ribozyme
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR221-SLC35G6_STOP

    Plasmid

    #161262

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC35G6 (SLC35G6 Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 15, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pLX307/PTPN11

    Plasmid

    #140941

    Purpose

    expresses PTPN11

    Depositor

    Kimberly Stegmaier

    Article

    Cremer et al Cancer Discov. 2020 Feb;10(2):214-231. d

    Insert

    PTPN11 protein tyrosine phosphatase non-receptor type 11 [ Homo sapiens (human) ] (PTPN11 Human)
    

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. p18S.2 tag

    Plasmid

    #51731

    Purpose

    transcribes truncated transcript containing 18S and hybridization tag

    Depositor

    Vincent Mauro

    Article

    Burman et al Nucleic Acids Res. 2012 Sep;40(16):8085-

    Insert

    18S
    

    Available Since

    July 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28a-cMSPdH45

    Plasmid

    #199767

    Purpose

    Expresses split-intein fused MSPdH45 for circularization during expression (internal His-Tag)

    Depositor

    Franz Hagn

    Article

    Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

    Insert

    cMSPdH45
    

    Tags

    His6-Tag

    Expression

    Bacterial

    Mutation

    internal His-Tag

    Available Since

    July 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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