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pCMV_mGL_PP7

Plasmid

#232440

Purpose

expression plasmid to produce mGreenLantern mRNA with a PP7 aptamer in the 3' UTR

Depositor

Gil Westmeyer

Article

Geilenkeuser et al Cell, April 2025. doi: 10.1016/j.cell.20

Insert

mGreenLantern

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pVAX1-BMP2

Plasmid

#137909

Purpose

Constitutive mammalian overexpression vector for human bone morphogenetic protein 2 cDNA

Depositor

Georg Feichtinger

Article

Feichtinger et al Eur Cell Mater. 2014 Feb 19;27:166-84; d

Insert

BMP2 (BMP2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pT077

Plasmid

#137879

Purpose

Donor vector for integration into the human AAVS1 safe harbor locus of Doxicycline-inducible KRAB-dCas9-IRES-EGFP

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

TRE3G-KRAB-dCas9-IRES-EGFP-CAG-TetON-NeoR-SA

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-6XHis-human SUMO 1

Plasmid

#133770

Purpose

express 6Xhis- SUMO 1 protein

Depositor

Martine Roussel

Article

Tago et al Proc Natl Acad Sci U S A. 2005 May 24. 1

Insert

SUMO 1 (SUMO1 Human)

Use

Tags

6XHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pET29a-hNABP1

Plasmid

#128306

Purpose

Express hNABP1 (a.k.a hSSB2 or SOSSB2) in E. coli with a C-terminal His-tag

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

Insert

Human nucleic acid-binding protein 1 (hNABP1) (NABP1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_TNF-SBP-RpHLuorin2

Plasmid

#171722

Purpose

Synchronize trafficking of TNFa-RpHLuorin2

Depositor

Geert van den Bogaart

Article

Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

Insert

TNF (TNF Human)

Use

Tags

Streptavidin Binding Protein (SBP) and ratiometri…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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CMV-GEM-GECO1

Plasmid

#32442

Purpose

Mammalian expression of blue-green emission ratiometric genetically encoded Ca2+ indicator for optical imaging

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

GEM-GECO1

Use

Tags

Expression

Mammalian

Mutation

GCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…

Promoter

CMV

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pLIX403_Capture1_APOBEC_HA_P2A_mRuby

Plasmid

#183901

Purpose

Inducible lentiviral expression, TRE-gateway-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTA

Depositor

Eugene Yeo

Article

Brannan et al Nat Methods. 2021 May;18(5):507-519. doi

Type

Empty backbone

Use

Lentiviral; Gateway destination vector, doxycycli…

Tags

Capture1_APOBEC_HA_P2A_mRuby

Expression

Mammalian

Mutation

Promoter

TRE promoter, Tet ON

Available since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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TRE3G-CTCF-dRBR-HA-MND-zeo

Plasmid

#232802

Purpose

Inducible over expression of RNA-binding domain deficient form of CTCF.

Depositor

Chunliang Li

Article

Hyle et al Genome Biol. 2023 Jan 26;24(1):14. doi:

Insert

Ctcf-dRBR (Ctcf Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-Rheb-N153T

Plasmid

#19997

Depositor

Fuyuhiko Tamanoi

Article

Urano et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

Insert

Ras homolog enriched in brain 1 - N153T (RHEB Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Changed Asparagine 153 to Threonine

Promoter

Available since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only
pT076

Plasmid

#137880

Purpose

Donor vector for integration at the human AAVS1 safe harbor locus of Doxicycline-inducible dCas9-VPR-T2A-EGFP

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

TRE3G-dCas9-VPR-T2A-EGFP-CAG-TetON-NeoR-SA

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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lenti-EGFR-L858R-tmpknot-epegRNA

Plasmid

#214092

Purpose

Lentiviral vector expressing epegRNA to induce EGFR L858R mutation

Depositor

Ting Zhou

Article

Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

Insert

EGFR L858R epegRNA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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ECFP-IKB

Plasmid

#111193

Purpose

fluorescent fusion protein

Depositor

Johannes A. Schmid

Article

Schmid et al J Biol Chem. 2000 Jun 2;275(22):17035-42

Insert

IKB-alpha

Use

Tags

ECFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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lenti-KRAS-G12C-tmpknot-epegRNA

Plasmid

#214094

Purpose

Lentiviral vector expressing epegRNA to induce KRAS G12C mutation

Depositor

Ting Zhou

Article

Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

Insert

KRAS G12C epegRNA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TRAIL-R2

Plasmid

#61383

Purpose

Expresses TRAIL receptor 2

Depositor

Francis Chan

Article

Clancy et al Proc Natl Acad Sci U S A. 2005 Dec 13;10

Insert

TRAIL receptor 2 (TNFRSF10B Human)

Use

Tags

none

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only

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CMV-FlicR1

Plasmid

#74142

Purpose

Mammalian expression of FlicR1: A bright red fluorescent genetically encoded voltage indicator

Depositor

Robert Campbell

Article

Abdelfattah et al J Neurosci. 2016 Feb 24;36(8):2458-72. d

Insert

FlicR1

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

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lenti-KRAS-G13A-tmpknot-epegRNA

Plasmid

#214095

Purpose

Lentiviral vector expressing epegRNA to induce KRAS G13A mutation

Depositor

Ting Zhou

Article

Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

Insert

KRAS G13A epegRNA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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PB-TRE-GNL

Plasmid

#204499

Purpose

Expresses Glis1, Nanog and lin28a by tet-on promoter in mammalian cells

Depositor

Wataru Fujii

Article

Matsuya et al Sci Rep. 2024 Feb 8;14(1):3204. doi: 10.

Insert

Glis1 (Glis1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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lenti-GBA-N370S-nick sgRNA

Plasmid

#214096

Purpose

Lentiviral vector expressing nicking sgRNA to induce GBA N370S mutation using PE3

Depositor

Ting Zhou

Article

Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

Insert

KRAS G13A nicking sgRNA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-GG-hUBC-EBFP2

Plasmid

#212314

Purpose

Lentiviral backbone with EBFP2 reporter

Depositor

Andrew Wilson

Article

Werder et al Sci Adv. 2022 Jul 15;8(28):eabo6566. doi

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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