We narrowed to 16,532 results for: puromycin

1. lenti-SpCas9 puro

   Plasmid

   #104994

   Purpose

   This 3rd generation lentiviral construct delivers hSpCas9 and puromycin resistance.

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Insert

   KpnI-XhoI-BsrGI-NheI
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLenti_P2A_Puro

   Plasmid

   #211364

   Purpose

   Lentiviral expression vector for an insert of interest linked to a P2A-T2A puromycin sequence. Produces virus very efficiently. Can also be used for regular transfections

   Depositor

   Jacques Neefjes

   Article

   Middelburg et al Cell Rep. 2023 Dec 26;42(12):113516. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Jan. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLVX-TetOne-Puro-PSMD4

   Plasmid

   #202666

   Purpose

   Tet-ON 3rd generation expression vector with inducible expression of PSMD4 and a puromycin selection cassette

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   proteasome 26S subunit ubiquitin receptor, non-ATPase 4 (PSMD4 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLX302_FOXA1-V5

   Plasmid

   #70090

   Purpose

   Expression of human FOXA1 with C-terminal V5 tag, puromycin selection marker, 3rd generation lentiviral vector

   Depositor

   William Hahn

   Article

   Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

   Insert

   FOXA1 (FOXA1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PLVX_SPTA1_IGF2BP1_IRES_PURO

   Plasmid

   #100016

   Purpose

   To over-express IGF2BP1 in Adult Erythroblasts using tissue specific promoter with IRES for puromycin selection.

   Depositor

   Jeffery Miller

   Article

   de Vasconcellos et al Proc Natl Acad Sci U S A. 2017 Jul 11;11

   Insert

   Insulin like growth factor 2 mRNA binding protein 1 (IGF2BP1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   SPTA1 erythroid specific promoter

   Available Since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. AAVS1 CAG rtTA3 TauWT 2N4R-EGFP

   Plasmid

   #132389

   Purpose

   Exchange cassette containing inducible TauWT 2N4R-EGFP fusion, CAG-driven rtTA3, Puromycin resistance marker, targets AAVS1 site

   Depositor

   Gerold Schmitt-Ulms

   Article

   Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

   Insert

   human TauWT 2N4R-EGFP (MAPT Human, Synthetic)
   

   Use

   CRISPR and Cre/Lox

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   TREtight

   Available Since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. LLP249 pGK-dCas9-DNMT3a V3

   Plasmid

   #100938

   Purpose

   dCas9 and DNMT WT driven by pGK promoter and Puromycin driven by EF1a

   Depositor

   Ryan Lister

   Article

   Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

   Insert

   dCas9, DNMT3a catalytic domain (DNMT3A S.pyogenes, Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA and 3xTy1

   Expression

   Mammalian

   Mutation

   D10A, H840A for dCas9

   Available Since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Lenti-RNF2-ngRNA+41_EF1a-puroR

   Plasmid

   #207358

   Purpose

   Lentiviral transfer plasmid encoding hU6-driven expression of a RNF2 nicking guide RNA and EF1a-driven puromycin resistance gene.

   Depositor

   Marianne Carlon

   Article

   Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

   Insert

   RNF2 +41 ngRNA
   

   Use

   Lentiviral

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pU6-sgCXCR4-2

   Plasmid

   #46917

   Purpose

   Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting endogenous CXCR4 gene

   Depositor

   Jonathan Weissman, Stanley Qi

   Article

   Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

   Inserts

   sgCXCR4 -2 (CXCR4 Human)
   Puromycin resistance and mCherry
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   CMV and U6

   Available Since

   Oct. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMx-puro-MGT

    Plasmid

    #111809

    Purpose

    Retroviral expression vector for direct cardiac reprogramming

    Depositor

    Li Qian

    Article

    Wang et al Circ Res. 2015 Jan 16;116(2):237-44. doi

    Insert

    Mef2c, Tbx5 and Gata4 (Gata4, Mef2c, Tbx5 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    viral LTR

    Available Since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pU6-sgCD71-2

    Plasmid

    #46918

    Purpose

    Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting endogenous CD71 gene

    Depositor

    Jonathan Weissman, Stanley Qi

    Article

    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

    Inserts

    sgCD71 -2 (TFRC Human)
    Puromycin resistance and mCherry
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    CMV and U6

    Available Since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. LCV2_EGFP

    Plasmid

    #155098

    Purpose

    Lentiviral backbone for cloning and expression of U6 driven gRNAs with Esp3I/BsmBI cloning sites, puromycin selection and EGFP expression

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSCAR_sgRNA_puro-mKate-lox5171

    Plasmid

    #162077

    Purpose

    3rd generation lentivirus sgRNA delivery backbone with Cre-removable mKate2 reporter and puromycin resistance

    Depositor

    Robert Manguso

    Article

    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

    Type

    Empty backbone

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Expression

    Mammalian

    Promoter

    U6, EF1a

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGAPTrap-mtagBFP2-IRESMuro

    Plasmid

    #82335

    Purpose

    Vector targeting the mtagBFP2 gene to the GAPDH locus of human cells. BFP is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.

    Depositor

    Ed Stanley

    Article

    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

    Insert

    mtagBFP2-IRESMuro
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PEA1-Puro-HEK3 CTT ins

    Plasmid

    #171996

    Purpose

    Delivers all prime editing (nickase) components targeting the HEK3 site for a CTT inserrtion, in a single, puromycin selectable plasmid

    Depositor

    Paul Thomas

    Article

    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

    Insert

    CbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA HEK3 CTT ins, hU6-sgRNA HEK3 +90
    

    Expression

    Mammalian

    Promoter

    CMV for Cas9, U6 for gRNAs

    Available Since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBRY-nuclear mCherry-IRES-PURO

    Plasmid

    #52409

    Purpose

    Constitutively expressed mammalian expression vector encoding nuclear mCherry fluorescent reporter. Puromycin resistance.

    Depositor

    Jacob Hanna

    Article

    Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

    Insert

    nuclear mCherry
    

    Expression

    Mammalian

    Available Since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CA-RIT-NFAT1 (PGK-Puro)

    Plasmid

    #63214

    Purpose

    Constitutively active NFAT1, mutated to interfere selectively with the NFAT:AP-1 interactio. Contains Puromycin selection marker

    Depositor

    Anjana Rao

    Article

    Martinez et al Immunity. 2015 Feb 6. pii: S1074-7613(15

    Insert

    CA-RIT-NFAT1 (Nfatc2 Mouse)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Amino acids 1-3 removed; CA: PASSGSSASF mutated t…

    Available Since

    July 22, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pQC MCS IRES Puro

    Plasmid

    #110343

    Purpose

    γ-Retroviral transfer vector for cloning and expressing your gene of interest. IRES-driven Puromycin selection.

    Depositor

    Sandra Martha Gomes Dias

    Article

    Adamoski et al Nat Struct Mol Biol. 2023 Dec;30(12):190

    Type

    Empty backbone

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pXPR_BRD003 sgKEAP1-1

    Plasmid

    #74985

    Purpose

    sgKEAP1-1, puromycin selection

    Depositor

    William Hahn

    Article

    Krall et al Elife. 2017 Feb 1;6:e18970. doi: 10.7554

    Insert

    KEAP1 (KEAP1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHL-EF1a-SphcCas9(D10A)-iP-A

    Plasmid

    #60600

    Purpose

    Expresses D10A mutant (nickase) of human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.

    Depositor

    Akitsu Hotta

    Article

    Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

    Inserts

    CRISPR Cas9 D10A
    puromycin resistance gene
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Changed Asp 10 to Ala, Codon usage optimized for …

    Available Since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge