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Plasmid#233871PurposeExpresses His-tagged Eggerthella lenta DSM 2243 El Vadh with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purificationDepositorInsertEl Vadh (Elen_0497 Eggerthella lenta)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD80ENSF12-Pct3-(–)-Eldh1(cHis6)-Accs(ElA2)
Plasmid#233870PurposeExpresses His-tagged Eggerthella lenta A2 (–)-Eldh1 with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purificationDepositorInsert(–)-Eldh1 (Elen_0511 Eggerthella lenta)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD80ENSF10-Pct3-(+)-Eddh(cHis6)-Accs(ElA2)
Plasmid#233869PurposeExpresses His-tagged Eggerthella lenta A2 (+)-Eddh with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purificationDepositorInsert(+)-Eddh (Elen_0501 Eggerthella lenta)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT1856
Plasmid#228328PurposeHomoserine lactone (HSL)-regulatable EGFP expressionDepositorInsertEGFP
UseSynthetic BiologyTagsExpressionYeastMutationPromoterHomoserine lactone (HSL)-regulatable synthetic pr…Available sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT1857
Plasmid#228329PurposeHomoserine lactone (HSL)-regulatable EGFP expressionDepositorInsertEGFP
UseSynthetic BiologyTagsExpressionYeastMutationPromoterHomoserine lactone (HSL)-regulatable synthetic pr…Available sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT1858
Plasmid#228330PurposeHomoserine lactone (HSL)-regulatable EGFP expressionDepositorInsertEGFP
UseSynthetic BiologyTagsExpressionYeastMutationPromoterHomoserine lactone (HSL)-regulatable synthetic pr…Available sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT1876
Plasmid#228331PurposeHomoserine lactone (HSL)-regulatable EGFP expressionDepositorInsertEGFP
UseSynthetic BiologyTagsExpressionYeastMutationPromoterHomoserine lactone (HSL)-regulatable synthetic pr…Available sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT1877
Plasmid#228332PurposeHomoserine lactone (HSL)-regulatable EGFP expressionDepositorInsertEGFP
UseSynthetic BiologyTagsExpressionYeastMutationPromoterHomoserine lactone (HSL)-regulatable synthetic pr…Available sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT1883
Plasmid#228333PurposeHomoserine lactone (HSL)-regulatable EGFP expressionDepositorInsertEGFP
UseSynthetic BiologyTagsExpressionYeastMutationPromoterHomoserine lactone (HSL)-regulatable synthetic pr…Available sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT1014
Plasmid#228298PurposeHomoserine lactone (HSL)-regulatable EGFP expressionDepositorInsertEGFP
UseSynthetic BiologyTagsExpressionYeastMutationPromoterHomoserine lactone (HSL)-regulatable synthetic pr…Available sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD70EN19-Pct5-GPA_25810/25800-(+)-Eldh1/2(GpDSM19378)
Plasmid#225305PurposeExpresses Gordonibacter pamelaeae DSM 19378 (+)-Eldh1 and (+)-Eldh2 in Eggerthella lenta DSM 2243 with a cumate inducible promoterDepositorInsert(+)-Eldh1, (+)-Eldh2
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD80ENSF27-Pct3-(+)-Eldh2(nHis6)(GpDSM19378)
Plasmid#233875PurposeExpresses His-tagged Gordonibacter pamelaeae DSM 19378 (+)-Eldh2 with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purificationDepositorInsert(+)-Eldh2 (GPA_25800 Gordonibacter pamelaeae DSM 19378)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pB-Puro-DEST (JDW 925)
Plasmid#229808PurposeA puromycin selectable, PiggyBac destination vector compatible with 4-way multi-site gateway cloning systemDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN-WPRE-bGH-DEST (JDW 1309)
Plasmid#229815PurposeA CAGGS driven, gateway compatible destination vector that contains a WPRE downstream of the destination cassette to stabilize transcriptsDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGLAST-DEST (JDW 1009)
Plasmid#229822PurposeA single site gateway compatible destination vector with an upstream GLAST promotor to drive expression in glial cells.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBeta-MHC-promoter DV (JDW 26)
Plasmid#229835PurposeA gateway compatible destination vector containing the murine beta-MHC promoter for embryonic and postnatal cardiomyocyte expression.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXMLC2-DEST (JDW 7)
Plasmid#229833PurposeA gateway compatible destination vector containing the Xenopous myosin light chain 2 promoter for embryonic and adult cardiomyocyte expression.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pME-MCS (JDW 455)
Plasmid#229832PurposeA gateway compatible middle entry clone containing an MCS (mutiple cloning site).DepositorInsertattL1/L2 flanked MCS
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceFeb. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN-DV-IRES-myr-BFP (JDW 491)
Plasmid#229834PurposeA CAGGS driven gateway compatible destination vector that contains an IRES-myristoylated-TagBFP for labeling the cell membraneDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pME-StayGoldc4-Giantin (JDW 1355)
Plasmid#229844PurposeA gateway compatible middle entry clone containing a V5 tagged n2StayGoldc4 flourescent proteinDepositorInsertn2StayGoldc4
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceFeb. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
p5E-CAGGS (JDW 912)
Plasmid#224472PurposeGateway compatible 5' entry clone containing the CAG promoter for constitutive expression of downstream constructs.DepositorInsertCAGGS Promoter
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
p3E-EF1a-pA (JDW 1222)
Plasmid#224526PurposeA Gateway compatible 3' entry clone containing rat EF-1a polyADepositorInsertEF-1a polyA
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
p5E_hs_Glast (JDW 1182)
Plasmid#224483PurposeGateway compatible 5' entry clone containing the Human GLAST promoterDepositorInsertGLAST promoter
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
p5E-EF1a (JDW 1164)
Plasmid#224473PurposeGateway compatible 5' entry clone containing the human EF1a promoterDepositorInsertEF1a promoter
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
p5E-hs_Cldn5 (JDW 1120)
Plasmid#224481PurposeGateway compatible 5' entry clone containing the Human Claudin 5 promoterDepositorInsertClaudin-5 promoter
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME AmCyan (JDW 1151)
Plasmid#224485PurposeGateway compatible middle entry clone containing AmCyan cytosolic blue flourescent reporterDepositorInsertAmCyan
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME V5 mScarlet-I (JDW 1323)
Plasmid#224487PurposeGateway compatible middle entry clone containing V5 tagged m-Scarlet-I (Cytosolic far red fluorescent reporter)DepositorInsertV5-mScarlet-I
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-mCerulean3-TUBB1A (JDW 1367)
Plasmid#224503PurposeGateway compatible middle entry clone containing mCerulean3 fused to TUBB1A for visualizing tubulin in cellsDepositorInsertmCerulean3-TUBB1A
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-LexA-mODC (no LexOp) (JDW 937)
Plasmid#224504PurposeGateway compatible middle entry clone containing a destabilized LexA transactivatorDepositorInsertLexA-mODC
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-myc-BirA (JDW 1210)
Plasmid#224509PurposeGateway compatible middle entry clone containing a Myc tagged BirADepositorInsertMyc-BirA
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-Myc-BioID2-MCS1 (JDW 1095)
Plasmid#224510PurposeGateway compatible middle entry clone containing a Myc-tagged BIOID2 constructDepositorInsertMyc-BioID2
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
p3E-H2A_mCherry_SV40pA (JDW 967)
Plasmid#224528PurposeA Gateway compatible 3' entry clone containing an H2A mCherry fusion followed by SV40 late polyADepositorInsertH2A-mCherry-SV40pA
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
p3E-V5-mClover3-pA (JDW 1318)
Plasmid#224530PurposeA Gateway compatible 3' entry clone containing a V5 tagged mClover3 followed by a polyADepositorInsertV5-mClover3-pA
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
p3E-IRES-mTagBFP2-pA (JDW 1361)
Plasmid#224535PurposeA Gateway compatible 3' entry clone containing an IRES-FLAG-NLS-mTagBFP2-pADepositorInsertIRES-FLAG-NLS-mTagBFP2-pA
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
p3E-IRES-(n2)StayGold(c4)-pA (JDW 1364)
Plasmid#224537PurposeA Gateway compatible 3' entry clone containing IRES-(n2)StayGold(c4)-pADepositorInsertIRES-(n2)StayGold(c4)-pA
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-Lifeact- mScarlet-HA (JDW 1247)
Plasmid#224538PurposeA Gateway compatible middle entry clone containing Lifeact-mScarlet-I (far red f-actin reporter)DepositorInsertLifeact-mScarlet-HA
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME H2B mCerulean (JDW 1150)
Plasmid#224543PurposeA Gateway compatible middle entry clone containing a histone H2B fusion to mCerulean to label the nucleusDepositorInsertH2B-mCerulean
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28Man
Plasmid#203399Purposeexo-beta mannosidase useful in enzymatic biotransformations of glycans and in coupled assays of various carbohydrate processing enzymesDepositorInsertman2A
UseTagsH6ExpressionBacterialMutationNonePromoterT7Available sinceAug. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEN-X-mPGK-NeoR-SV40 polyA-Z
Plasmid#218909Purposegolden gate cloningDepositorInsertmPGK-Neomycine-SV40 polyA
UseTagsExpressionMammalianMutation395T>CPromoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEN-X-mPGK-PuroR-SV40 polyA-Z
Plasmid#218908Purposegolden gate cloningDepositorInsertmPGK-PuroR-SV40 polyA
UseTagsExpressionMammalianMutation297T>CPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only