Addgene

We narrowed to 9,510 results for: Gnas

Showing: 3051 - 3100 of 9510 results

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pVEGFR2_TEV(N)_NES_TCS(Q'L)_FKBP_HA-dCas9(N)_P2A_Puro-WPRE

Plasmid

#101106

Purpose

Encodes FKBP-dCas9(N) fused to VEGFR2

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

VEGFR2-FKBP-dCas9(N)

Use

CRISPR and Synthetic Biology

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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px330BRG1 gRNA

Plasmid

#165585

Purpose

Insertion of BRG1 degron

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

BRG1 (Smarca4 Mouse)

Use

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pFlox c-SPT5_EYFP_FKBPF36V_Neo

Plasmid

#163872

Purpose

Acute Depletion of SPT5-EYFP

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

Spt5 (Supt5 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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CRY2(delta2-6)-mCherry

Plasmid

#104182

Purpose

Expresses fusion of CRY2(delta aa 2-6) with mCherry

Depositor

Bianxiao Cui

Article

Duan et al Nat Commun. 2017 Sep 15;8(1):547. doi: 1

Insert

CRY2(delta2-6)-mCherry

Use

Tags

Expression

Mammalian

Mutation

CRY2PHR with deletion of aa 2-6

Promoter

CMV

Available since

Dec. 19, 2017

Availability

Academic Institutions and Nonprofits only

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CRY2(neutral2-6)-mCherry

Plasmid

#104181

Purpose

Expresses fusion of CRY2(neutral aa 2-6) with mCherry

Depositor

Bianxiao Cui

Article

Duan et al Nat Commun. 2017 Sep 15;8(1):547. doi: 1

Insert

CRY2(neutral2-6)-mCherry

Use

Tags

Expression

Mammalian

Mutation

CRY2PHR K2S M3S D4G K5S K6S

Promoter

CMV

Available since

Dec. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pRRG159-foxD

Plasmid

#99068

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea foxD

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

foxD in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Tags

Expression

Mutation

Promoter

Available since

Aug. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pSP571

Plasmid

#139498

Purpose

CRISPR-Cas9 plasmid to generate double strand break in STL1 locus in S. cerevisiae. Expresses both Cas9 and STL1 sgRNA

Depositor

Serge Pelet

Article

Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

Insert

pGPD Cas9 / sgRNA (STL162)

Use

Tags

Expression

Yeast

Mutation

WT

Promoter

pGPD

Available since

June 12, 2020

Availability

Academic Institutions and Nonprofits only

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pFlox_N-BRG1_BSD_FKBPF36V_EYFP

Plasmid

#163874

Purpose

Acute Depletion of BRG1-EYFP

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

Brg1 (Smarca4 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-FLAG-Fos Y345A

Plasmid

#8970

Depositor

John Blenis

Article

Murphy et al Nat Cell Biol. 2002 Aug . 4(8):556-64.

Insert

c-Fos (Fos Rat)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Y345A

Promoter

Available since

Sept. 2, 2005

Availability

Academic Institutions and Nonprofits only
pBS mouse IRS4 promoter

Plasmid

#11445

Depositor

Ronald Kahn

Article

Tsuruzoe et al Mol Cell Biol. 2001 Jan . 21(1):26-38.

Insert

IRS-4 promoter (Irs4 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 3, 2006

Availability

Academic Institutions and Nonprofits only
EGFP-bSV-171-1792

Plasmid

#41661

Depositor

Elizabeth Luna

Article

Wulfkuhle et al J Cell Sci. 1999 Jul:112 (Pt 13):2125-36

Insert

supervillin (SVIL Bovine)

Use

Tags

EGFP

Expression

Mammalian

Mutation

amino acids 171-1792

Promoter

Available since

Dec. 12, 2012

Availability

Academic Institutions and Nonprofits only
pAAV_2pabPAC

Plasmid

#158969

Purpose

2P-activatable bPAC for AAV

Depositor

Michiyuki Matsuda

Article

Kinjo et al ACS Chem Biol. 2020 Oct 19. doi: 10.1021

Insert

2pabPAC

Use

AAV

Tags

Expression

Mutation

Promoter

CMV

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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EGFP-bSV-831-1281

Plasmid

#40962

Depositor

Elizabeth Luna

Article

Wulfkuhle et al J Cell Sci. 1999 Jul:112 (Pt 13):2125-36

Insert

supervillin (SVIL Bovine)

Use

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 1-830 and 1282-1792

Promoter

Available since

Dec. 12, 2012

Availability

Academic Institutions and Nonprofits only
EGFP-bSV-1282-1792

Plasmid

#40960

Depositor

Elizabeth Luna

Article

Wulfkuhle et al J Cell Sci. 1999 Jul:112 (Pt 13):2125-36

Insert

supervillin (SVIL Bovine)

Use

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 1-1281

Promoter

Available since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only
pZF-ADD-L

Plasmid

#127477

Purpose

coding sequence for synthetic zinc finger ZF-ADD-L

Depositor

Sebastian Maerkl

Article

Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

Insert

ZF-ADD synthetic transcription factor fused with PDZ cognate peptide ligand (VKESLV)

Use

Cell-free transcription–translation

Tags

PDZ cognate peptide ligand (VKESLV)

Expression

Mutation

Promoter

T7

Available since

Nov. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pmFgf7

Plasmid

#31237

Depositor

Cliff Ragsdale

Article

Assimacopoulos et al J Neurosci. 2003 Jul 23. 23(16):6399-403

Insert

Fgf7 (Fgf7 Mouse)

Use

Cdna for in situ hybridization

Tags

Expression

Mutation

Promoter

Available since

Sept. 15, 2011

Availability

Academic Institutions and Nonprofits only
pNLRP11minor.MYC

Plasmid

#131195

Purpose

Mammalian expression of human NLRP11 188A

Depositor

Thomas Kufer

Article

Ellwanger et al J Biol Chem. 2018 Jan 4. pii: RA117.0001

Insert

NLRP11 188A (NLRP11 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

188A

Promoter

pCMV

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pBDKRB2_TCS(Q’G)_NLS-HA-dCas9(C)_T2A_DHFR-PCP-VP64

Plasmid

#101110

Purpose

Encodes dCas9(C) with DHFR-PP7-VP64 fused to BDKRB2

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

BDKRB2-dCas9(C)_DHFR-PCP-VP64

Use

CRISPR and Synthetic Biology

Tags

Flag and HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pSP64TS human TGFbeta-IIR (DM#169)

Plasmid

#15012

Depositor

Douglas Melton

Article

Reilly et al Cell. 1996 Sep 6. 86(5):743-54.

Insert

TGF beta type II receptor (TGFBR2 Human)

Use

Sp6 expression for use in xenopus

Tags

Expression

Mutation

Promoter

Available since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only
pMX Vav1 Y3F/DH* (L213Q) uGFP

Plasmid

#14559

Depositor

Joan Brugge

Article

Wilsbacher et al Cell Commun Signal. 2006 . 4():5.

Insert

Vav1 (VAV1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Y3F (Y142, Y160, Y174)/DH* (L213Q)

Promoter

Available since

April 12, 2007

Availability

Academic Institutions and Nonprofits only
SPDEF_pet28a

Plasmid

#131643

Purpose

Bacterial expression of ETS gene SPDEF

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

SPDEF (SPDEF Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pJH2933

Plasmid

#132363

Purpose

Prgef-1 INS-3::GFP unc-54 3' UTR C.elegans pan-neural expression of INS-3::GFP

Depositor

Mei Zhen

Article

Hung et al Development. 2014 Apr;141(8):1767-79. do

Insert

INS-3 (ins-3 Nematode)

Use

Tags

GFP

Expression

Worm

Mutation

Promoter

Prgef-1

Available since

Dec. 11, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pcDNA3-FLAG-Fos F343A

Plasmid

#8969

Depositor

John Blenis

Article

Murphy et al Nat Cell Biol. 2002 Aug . 4(8):556-64.

Insert

c-Fos (Fos Rat)

Use

Tags

FLAG

Expression

Mammalian

Mutation

F343A

Promoter

Available since

Sept. 2, 2005

Availability

Academic Institutions and Nonprofits only
pMX Vav1 Y3F/NSH3* (P651L) uGFP

Plasmid

#14562

Depositor

Joan Brugge

Article

Wilsbacher et al Cell Commun Signal. 2006 . 4():5.

Insert

Vav1 (VAV1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Y3F/NSH3* (P651L)

Promoter

Available since

April 12, 2007

Availability

Academic Institutions and Nonprofits only
pCI Flag Smad3 NL WT

Plasmid

#27040

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

aa 1-226

Promoter

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pGCaMP5

Plasmid

#78705

Purpose

GCaMP5 under the Toxoplasma SAG1 5' UTR. No Toxoplasma selectable marker

Depositor

Sebastian Lourido

Article

Sidik et al J Biol Chem. 2016 Apr 29;291(18):9566-80

Insert

GCaMP5

Use

Tags

Expression

Mutation

Promoter

SAG1 5' UTR

Available since

May 27, 2016

Availability

Academic Institutions and Nonprofits only

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Wnt1 Non-STOP

Plasmid

#35887

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt1 (WNT1 Human)

Use

Entry vector

Tags

Expression

Mutation

None

Promoter

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT Draper-ITAM only-GFP

Plasmid

#126656

Purpose

Inducible expression of Draper-ITAM only-GFP mutant in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Draper with Cytoplasmic ITAM residues Tyr all others mutated to Phe

Use

Tags

Expression

Insect

Mutation

Cytoplasmic ITAM residues Tyr all others mutated …

Promoter

Available since

June 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Mad WT

Plasmid

#22996

Depositor

Edward De Robertis

Article

Eivers et al PLoS One. 2009 . 4(8):e6543.

Insert

Mad (Mad Fly)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
pBW214J115-Amp32C

Plasmid

#78649

Purpose

pSB3K3 carrying J115-30hrpR-32hrpS-t-hrpL-30gfp-t

Depositor

Baojun Wang

Article

Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

Insert

J115-30hrpR-32hrpS-t-hrpL-30gfp-t

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

see insert

Available since

July 27, 2016

Availability

Academic Institutions and Nonprofits only
pES1002

Plasmid

#21789

Depositor

Richard Yu

Article

Yu et al Nature. 2008 Dec 11. 456(7223):755-61.

Insert

Fus3-as2 (FUS3 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

contains 5' 1kb upstream UTR/promoter Q93…

Promoter

Available since

Oct. 1, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
Wnt10A Non-STOP

Plasmid

#35902

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt10A (WNT10A Human)

Use

Entry vector

Tags

Expression

Mutation

None

Promoter

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pJH2256

Plasmid

#132365

Purpose

Prgef-1 INS-6::GFP unc-54 3' UTR C.elegans pan-neural expression of INS-6::GFP

Depositor

Mei Zhen

Article

Hung et al Development. 2014 Apr;141(8):1767-79. do

Insert

INS-6 (ins-6 Nematode)

Use

Tags

GFP

Expression

Worm

Mutation

Promoter

Prgef-1

Available since

Jan. 8, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pJH2255

Plasmid

#132364

Purpose

Prgef-1 INS-4::GFP unc-54 3' UTR C.elegans pan-neural expression of INS-4::GFP

Depositor

Mei Zhen

Article

Hung et al Development. 2014 Apr;141(8):1767-79. do

Insert

INS-4 (ins-4 Nematode)

Use

Tags

GFP

Expression

Worm

Mutation

Promoter

Prgef-1

Available since

Oct. 7, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pUC57-Kan_AVR1-CO39

Plasmid

#123654

Purpose

Level 0 Golden-Gate compatible vector

Depositor

Sophien Kamoun

Article

Petit-Houdenot et al Mol Plant Microbe Interact. 2020 Aug;33(

Insert

AVR1-CO39

Use

Golden gate

Tags

Expression

Mutation

Promoter

Available since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-M2-TLR2-FW/AA

Plasmid

#12293

Depositor

Bruce Beutler

Article

Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

TLR2 (Tlr2 Mouse)

Use

Tags

FLAG (FLAG-M2)

Expression

Mammalian

Mutation

M82I, H202Y, F774A and W775A; signal peptide (aa1…

Promoter

Available since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only
pMX Vav1 Y3F/SH2* (R696K) uGFP

Plasmid

#14565

Depositor

Joan Brugge

Article

Wilsbacher et al Cell Commun Signal. 2006 . 4():5.

Insert

Vav1 (VAV1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Y3F/SH2* (R696K)

Promoter

Available since

April 12, 2007

Availability

Academic Institutions and Nonprofits only
HL 3722 (=HL 716 + pHL 404)

Plasmid

#30084

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Insert

pLtetO-1:MicC, pLlacO-1:hfq, pLtetO-1:ompC::gfp

Use

Synthetic Biology

Tags

GFP

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 28, 2011

Availability

Academic Institutions and Nonprofits only
pGEMT-NRTN

Plasmid

#119896

Purpose

Mouse NRTN In situ hybridization probe

Depositor

Wenqin Luo

Article

Fleming et al Elife. 2015 Apr 2;4:e06828. doi: 10.7554

Insert

neurturin probe (Nrtn Mouse)

Use

In situ hybridization probe

Tags

Expression

Mutation

Promoter

Available since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
beta4-nAChR (DPM negative control)

Plasmid

#86651

Purpose

expression of control shRNA

Depositor

Jaime Modiano

Article

Oloris et al Toxicol Appl Pharmacol. 2010 Feb 1;242(3

Insert

CHRNB4 (control)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMX Vav1 Y3F/CSH3* (P833L) uGFP

Plasmid

#14566

Depositor

Joan Brugge

Article

Wilsbacher et al Cell Commun Signal. 2006 . 4():5.

Insert

Vav1 (VAV1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Y3F/CSH3* (P833L)

Promoter

Available since

April 12, 2007

Availability

Academic Institutions and Nonprofits only
pBW211J109-Amp32C

Plasmid

#78646

Purpose

pSB3K3 carrying J109-30hrpR-32hrpS-t-hrpL-30gfp-t

Depositor

Baojun Wang

Article

Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

Insert

J109-30hrpR-32hrpS-t-hrpL-30gfp-t

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

see insert

Available since

July 27, 2016

Availability

Academic Institutions and Nonprofits only
pNB690

Plasmid

#60134

Purpose

Yellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by MET17 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

YeLuc

Use

Tags

ClpXP degron and Nuclear localization signal (NLS…

Expression

Yeast

Mutation

Promoter

MET17pr

Available since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pClneoFLAG mouse RASSF2

Plasmid

#37017

Depositor

Yutaka Hata

Article

Ikeda et al Sci Signal. 2009 Sep 29;2(90):ra59.

Insert

RASSF2 (Rassf2 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

D72G and S127F

Promoter

CMV

Available since

Aug. 13, 2012

Availability

Academic Institutions and Nonprofits only
pFastBac-LacI-LAP

Plasmid

#114034

Purpose

Production of baculovirus with LacI-LAP

Depositor

Geert Kops

Article

Hiruma et al Science. 2015 Jun 12;348(6240):1264-7. d

Insert

LacI-LAP

Use

Tags

Expression

Insect

Mutation

none

Promoter

CMV

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pRRG88-follistatin

Plasmid

#99066

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea follistatin

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

follistatin in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Tags

Expression

Mutation

Promoter

Available since

Aug. 22, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLJM1-NtermRGS2-GFP-CIBN

Plasmid

#112258

Purpose

Expresses alpha-helix component of opto-RGS2 in mammalian cells

Depositor

Brian Chow

Article

Hannanta-Anan et al ACS Synth Biol. 2018 Jun 15;7(6):1488-14

Insert

NtermRGS2-GFP-CIBN

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
XE132 XFz7-CS2P+

Plasmid

#16792

Depositor

Randall Moon

Article

Brown et al Dev Growth Differ. 2000 Aug. 42(4):347-5

Insert

XFz7-CS2P+ (fzd7.L Frog)

Use

Xenopus expression

Tags

Expression

Mutation

Promoter

Available since

March 14, 2008

Availability

Academic Institutions and Nonprofits only
EGFP-bSV-831-1792

Plasmid

#40961

Depositor

Elizabeth Luna

Article

Wulfkuhle et al J Cell Sci. 1999 Jul:112 (Pt 13):2125-36

Insert

supervillin (SVIL Bovine)

Use

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 1-830

Promoter

Available since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only
EGFP-bSV-1010-1792

Plasmid

#40959

Depositor

Elizabeth Luna

Article

Wulfkuhle et al J Cell Sci. 1999 Jul:112 (Pt 13):2125-36

Insert

supervillin (SVIL Bovine)

Use

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 1-1009

Promoter

Available since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only

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