We narrowed to 7,229 results for: gan
-
Plasmid#113083PurposeEnhanced C. elegans Cas9 vector. Ccas9LacZ contains a lacZ coding sequence, two Cas9 and two type-IIs restriction enzyme recognition sites next to the U6 promoter and gRNA sequences.DepositorTypeEmpty backboneUseCRISPRExpressionWormAvailable sinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only
-
pGC629
Plasmid#71364Purposeproximal somatic gonad expression of DAF-16 in C.elegansDepositorAvailable sinceDec. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIK208
Plasmid#65630PurposesgRNA targeting dpy-10 (Arribere et al., 2014) expressed from a C. elegans U6 promoter (Friedland et al., 2013), in a "flipped and extended" sgRNA backbone (Chen et al., 2013)"DepositorInsertU6 promoter::dpy-10 sgRNA with a "flipped and extended" sgRNA backbone
UseCRISPRExpressionWormAvailable sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SNF-5 pXOOM
Plasmid#48697PurposeExpressed SNF-5 in Xenopus oocytes or mammalian cellsDepositorInsertsnf-5 (snf-5 Nematode)
UseXenopus oocyte expressionExpressionMammalianMutationK42E (when compared to NP_496735.1)PromoterT7Available sinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
F5-bio-His
Plasmid#53426PurposeExpresses full-length Coagulation factor V precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertF5 (F5 Human)
TagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
NID1-bio-His
Plasmid#52068PurposeExpresses full-length Nidogen-1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertNID1 (NID1 Human)
TagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMB63
Plasmid#47945PurposeExpresses C. elegans optimized Cas9 from the eef-1A.1 (eft-3) promoterDepositorPublicationInsertCas9
UseCRISPRTags3xFLAG, SV40 NLS, and egl-13 NLSExpressionWormPromotereef-1A.1 (eft-3)Available sinceDec. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
-
pMDJ10 - pEXP[Peft-3 | tagBFP2 (NLS) | tbb-2 UTR]
Plasmid#159798PurposeExpression construct with ubiquitous expression to test fluorescence level on microscopeDepositorInsertpEXP[Peft-3 | tagBFP2 (NLS) | tbb-2 UTR]
ExpressionWormMutationNot applicableAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMDJ12 - pEXP[Peft-3 | mNeonGreen (NLS) | tbb-2 UTR]
Plasmid#159799PurposeExpression construct with ubiquitous expression to test fluorescence level on microscopeDepositorInsertpEXP[Peft-3 | mNeonGreen (NLS) | tbb-2 UTR]
ExpressionWormMutationNot applicableAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA_RGG-GFP-RGG_withTEVsite
Plasmid#124939PurposeExpresion of RGG_GFP-RGGDepositorInsertRGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
TagsGFPExpressionMammalianMutationTEV site between RGG and EGFPAvailable sinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET_MBP-RGG-GFP-RGG
Plasmid#124949PurposeExpression of MBP-RGG-GFP-RGGDepositorInsertMBP-RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
TagsEGFP, His, and MBPExpressionBacterialAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
KG#121
Plasmid#110879PurposeExpresses the C. elegans unc-31 cDNA pan-neuronallyDepositorInsertsrab-3 promoter
unc-31 cDNA
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
ExpressionBacterialMutationMissing first 408bpAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
L3786
Plasmid#1593DepositorTypeEmpty backboneTagslet858enh>>, let858enh<<, let858-455,…ExpressionWormAvailable sinceMarch 7, 2005AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLV 4xSTAR-mNeonGreen-NLS-blast
Plasmid#136258PurposeFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activityDepositorInsertstem cell Ascl2 reporter, 4 repeats
UseLentiviralTagsmNeonGreen-NLSExpressionMammalianAvailable sinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOX + kqt1
Plasmid#16136DepositorInsertCaenorhabditis elegans potassium channel (kqt-1 Nematode)
UseXenopus oocyte expressionMutationN/AAvailable sinceJan. 10, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJRoC30_VP8H
Plasmid#188062PurposePROSS-designed versatile peroxidase 8HDepositorInsertPROSS-designed versatile peroxidase from Ganoderma sp. 10597_SS1
TagsS. cerevisiae native α-factor prepro-leader signa…ExpressionYeastMutation38 PROSS mutations, described in publicationAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMDJ21 - pEXP [Psmu-1 | eFlp recombinase (PATCs) | smu-1 UTR]
Plasmid#159810PurposeOptimized enzyme for genome editing in C. elegansDepositorInsertpEXP [Psmu-1 | eFlp recombinase (PATCs) | smu-1 UTR]
ExpressionWormMutationNot applicableAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOD2045-intControl
Plasmid#100005PurposeMosSCI targeting vector expressing an ubiquitin ligase adaptor ZIF-1, serving as a control for pOD2044-intDEG. Expression is controlled by Pelt-2 (intestine specific).DepositorInsertsUseTargeting vector for mos1 transposon mediated sin…Available sinceSept. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGDP4 tet(A)
Plasmid#112887PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Plac promoter.DepositorInserttet(A)
Tags6xHisExpressionBacterialMutationSequence was codon optimized for E. coli K12 usin…PromoterPlacAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only