We narrowed to 8,924 results for: pcDNA3

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pcDNA3-ER-ExRai-CKAR2

Plasmid

#236099

Purpose

Enhanced excitation-ratiometric biosensor for monitoring Protein Kinase C activity in living cells, targeted to ER surface.

Depositor

Jin Zhang

Article

Su et al Nat Chem Biol. 2024 Oct 11. doi: 10.1038

Insert

CYP450-ExRai-CKAR2

Tags

N-terminal 27 amino acids from CYP450

Expression

Mammalian

Promoter

CMV

Available Since

May 23, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyso-Digda

Plasmid

#236102

Purpose

Lysosome-targeted FRET-based biosensor for monitoring diacylglycerol (DAG) dynamics in living cells.

Depositor

Jin Zhang

Article

Su et al Nat Chem Biol. 2024 Oct 11. doi: 10.1038

Insert

Lyso-Digda (LAMP1 Human, Synthetic)

Tags

Full-length LAMP1

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-Siah2

Plasmid

#122041

Purpose

express Siah2

Depositor

Ze'ev Ronai

Insert

Siah2 (Siah2 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

April 22, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyso-INPP5E

Plasmid

#236004

Purpose

Lysosome-targeted inositol polyphosphate 5 phosphatase (INPP5E); hydrolyzes 5-phosphate in PI(3,4,5)P3 and PI(4,5)P2.

Depositor

Jin Zhang

Article

Sahan et al Proc Natl Acad Sci U S A. 2025 Apr;122(1

Insert

Lyso-INPP5E (LAMP1, INPP5E Human, Synthetic)

Tags

Full-length LAMP1 and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hMOR-rLuc8

Plasmid

#254716

Purpose

Expresses human wildtype mu opioid receptor (MOR) with C-terminal rLuc8. Vector used for G protein recruitment or 'nuc-BRET' assay.

Depositor

Cornelius Gati

Article

Khan et al Nature. 2025 Dec;648(8094):755-763. doi:

Insert

human mu opioid receptor (OPRM1 Human)

Tags

FLAG and rLuc8

Expression

Mammalian

Promoter

CMV

Available Since

April 27, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TLR8-YFP

Plasmid

#13024

Depositor

Doug Golenbock

Insert

Toll-Like Receptor 8 (TLR8 Human)

Tags

YFP

Expression

Mammalian

Available Since

Nov. 3, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+-Tornado-nLuc

Plasmid

#212611

Purpose

For expression of a circular nLuc mRNA. To express your gene of interest clone into the BsiWI, SacII sites and add the GCCATGTcTATGTGG 5' of the SacII site.

Depositor

Samie Jaffrey

Article

Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2

Insert

Tornado-CVB3-nLuc

Use

Luciferase

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-Myc3-Nrf2

Plasmid

#21555

Purpose

Mammalian expression of human Nrf2 with Myc tag

Depositor

Yue Xiong

Article

Furukawa et al Mol Cell Biol. 2005 Jan . 25(1):162-71.

Insert

nuclear factor (erythroid-derived 2)-like 2 (NFE2L2 Human)

Tags

myc3

Expression

Mammalian

Available Since

July 1, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PM-ExRai-AktAR2

Plasmid

#184050

Purpose

Plasma membrane-targeted ExRai-AktAR2

Depositor

Jin Zhang

Article

Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

Insert

PM-ExRai-AktAR2

Tags

N-terminal targeting sequence from Lyn kinase

Expression

Mammalian

Promoter

CMV

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pY010 (pcDNA3.1-hAsCpf1)

Plasmid

#69982

Purpose

Expresses humanized AsCpf1

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

hAsCpf1

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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Human V5-IRF3-pcDNA3

Plasmid

#32713

Depositor

Saumen Sarkar

Article

Zhu et al J Immunol. 2010 May 15;184(10):5768-76.

Insert

IRF3 (IRF3 Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3-BaLgp120-8his

Plasmid

#100925

Purpose

Mammalian expression plasmid for gp120 subunit from the BaL HIV-1 strain

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (BaL) Env

Tags

CD5 leader sequence and Purification tag after K5…

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available Since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3/Flag-METTL3

Plasmid

#53739

Purpose

For expression of METTL3 (methyltransferase-like 3) in human cells

Depositor

Chuan He

Article

Liu et al Nat Chem Biol. 2014 Feb;10(2):93-5. doi:

Insert

Methyltransferase-like 3 (METTL3 Human)

Tags

FLAG tag

Expression

Mammalian

Promoter

T7

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1.Gαi2-LgB91

Plasmid

#134358

Purpose

Nanoluc complementation assay. Expression of Gαi2 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi2. Addition of the HA epitope at N terminus of Gαi2.

Depositor

Julien Hanson

Article

Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

Insert

Gαi2-LgB91 (GNAI2 Human)

Tags

HA

Expression

Bacterial and Mammalian

Promoter

T7

Available Since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc-CUL3

Plasmid

#19893

Depositor

Yue Xiong

Article

Ohta et al Mol Cell. 1999 Apr . 3(4):535-41.

Insert

cullin 3 (CUL3 Human)

Tags

3xmyc

Expression

Mammalian

Available Since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Q842gp120-8his

Plasmid

#100932

Purpose

Mammalian expression plasmid for gp120 subunit from the Q842.d12 HIV-1 strain

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (Q842.d12) Env

Tags

CD5 leader sequence and Purification tag after K5…

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available Since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-INSR-V5/HIS

Plasmid

#201980

Purpose

expression of human INSR receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

INSR receptor tyrosine kinase (INSR Human)

Tags

V5, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Gβγ-BERKY3

Plasmid

#158219

Purpose

BRET biosensor for detection of free Gβγ in mammalian expression vector pcDNA3.1(+)

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

Lyn11-Nluc-ER/K linker-3xYFP-GRK3ct

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3-sACE2(WT)-Fc(IgG1)

Plasmid

#145163

Purpose

Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1)

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Tags

Fc region of human IgG1

Expression

Mammalian

Mutation

Extracellular, soluble protease domain (a.a. M1-D…

Promoter

CMV

Available Since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3 GFP-h-PKD2

Plasmid

#83451

Purpose

GFP-tagged PC2 for Eukaryotic expression (883)

Depositor

Thomas Weimbs

Article

Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

Insert

Polycystin-2 (PKD2 Human)

Tags

GFP

Expression

Mammalian

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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