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FUGW-Mito-TurboID

Plasmid

#200963

Purpose

3rd gen lentiviral plasmid for expressing mitochondria localized TurboID with EGFP

Depositor

Keriann Backus

Article

Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

Insert

Mito-TurboID

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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His-Tev-UbE1

Plasmid

#186804

Purpose

Codon-optimized bacterial expression plasmid. Expresses ubiquitin activating enzyme His-Tev-UbE1.

Depositor

Lalit Deshmukh

Article

Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

Insert

His-Tev-UbE1 (UBA1 Human)

Tags

His6-Tev

Expression

Bacterial

Available Since

March 23, 2023

Availability

Academic Institutions and Nonprofits only

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pET 28 full-length human PARP1

Plasmid

#192635

Purpose

Expresses human PARP1 with an N-terminal His-tag

Depositor

John Pascal

Article

Langelier et al J Biol Chem. 2008 Feb 15;283(7):4105-14.

Insert

Human PARP1 (PARP1 Human)

Tags

histidine-tag

Expression

Bacterial

Mutation

Polymorphism A762

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pSBbi G418 Hu CD19

Plasmid

#183247

Purpose

Expression of human CD19 antigen in a Sleeping Beauty transposon vector

Depositor

Alexander McLellan

Article

Dobson et al Immunol Cell Biol. 2023 Aug 16. doi: 10.

Insert

CD19 (CD19 Human)

Expression

Mammalian

Available Since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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Halo-Rab6A

Plasmid

#190171

Purpose

Expresses HaloTag-Rab6A in mammalian cells.

Depositor

Anna Akhmanova

Article

Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

Insert

HaloTag-Rab6A

Tags

HaloTag

Expression

Mammalian

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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iRFP-H2A

Plasmid

#158691

Purpose

This plasmid encodes an iRFP fused to H2A histone nuclear marker. It has CMV promoter driving the expression of iRFP fused to H2A. It has no selection markers

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

iRFP670

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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ZsGreen1-cMYC/pLVX-Puromycin

Plasmid

#180278

Purpose

Plasmid encoding human c-MYC for reporter assays. Polycistronic puromycin selectable lentiviral vector (unmodified, from TAKARA BIO).

Depositor

Andre Catic

Article

Moree et al Cancers (Basel). 2022 Feb 4;14(3). pii:

Insert

ZsGreen1-cMYC fusion protein MYC (MYC Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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MAC_N_IRF1

Plasmid

#167760

Purpose

Expresses IRF1 in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

IRF1 (IRF1 Human)

Tags

MAC

Expression

Mammalian

Mutation

no mutations

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pCMMP-MCS-IRES-eGFP-P2A-iCas9

Plasmid

#178533

Purpose

For genome editing: EGFP-inducible Caspase9 knock-in cassette which allows ablation of target cells

Depositor

Ryoji Yao

Article

Okamoto et al STAR Protoc. 2021 Sep 16;2(4):100780. do

Insert

iCaspase9 (CASP9 Human)

Expression

Mammalian

Mutation

FKBP12 F36 to V Truncated Caspase9 (135-416; lack…

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_IgKchL_HA_HaloTag9_myc_PDGFRtmb

Plasmid

#175544

Purpose

Expression of HaloTag9 at the outer leaflet of the plasma membrane of mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

IgKchL_HA-HaloTag9-myc_PDGFRtmb

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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