We narrowed to 17,090 results for: URE

1. pX330 Ctnnb1.2

   Plasmid

   #59912

   Purpose

   pX330 backbone expressing sgRNA targeting Ctnnb1 to edit mouse beta-Catenin. Expresses Cas9 from CBh promoter

   Depositor

   Tyler Jacks

   Article

   Xue et al Nature. 2014 Aug 6. doi: 10.1038/nature1

   Insert

   Ctnnb1 (Ctnnb1 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRSV-CaMKIV(1-313)

   Plasmid

   #45063

   Purpose

   Expression of constit active CaMKIV

   Depositor

   Richard Maurer

   Article

   Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

   Insert

   Cam Kinase IV(1-313) (Camk4 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   truncated at amino acid 313*

   Promoter

   RSV

   Available since

   Aug. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDEST-mCherry-LacR-BRCA1 1366-1437

   Plasmid

   #71118

   Purpose

   mammalian expession of mCherry-LacR-BRCA1 1366-1437

   Depositor

   Daniel Durocher

   Article

   Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

   Insert

   BRCA1 (BRCA1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   contains amino acids 1366-1437 of BRCA1

   Promoter

   CMV/TO

   Available since

   April 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGEX4T3-HA-ERK2 D321N

   Plasmid

   #53202

   Purpose

   Expresses GST and HA tagged human ERK2 D321N from bacterial expression vector

   Depositor

   Christopher Counter

   Article

   Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Insert

   ERK2 (MAPK1 Human)
   

   Use

   Tags

   GST and HA

   Expression

   Bacterial

   Mutation

   D321N

   Promoter

   Available since

   June 24, 2014

   Availability

   Academic Institutions and Nonprofits only

5. LITE2.0_pAAV_hSyn_TALE(Grm2)-GS-CIB1(NLS*,∆318-334)_WPRE_bGHpA

   Plasmid

   #47456

   Purpose

   LITE2.0 TALE-CIB1. Changes from LITE1.0: glycine-serine linker, mutated endogenous NLS, aa318-334 deletion. This particular TALE targeted to Grm2. Synapsin promoter for neuronal expression.

   Depositor

   Feng Zhang

   Article

   Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

   Insert

   TALE(Grm2)-GS-CIB1(NLS*,∆318-334)
   

   Use

   AAV; Tale

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn human synapsin (mammalian neuronal expressio…

   Available since

   Nov. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1/NDUFS8/myc-His

   Plasmid

   #127489

   Purpose

   Expresses myc-tagged NDUFS8 in mammalian cells

   Depositor

   Shashi Jain, Dieter Wolf

   Article

   Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

   Insert

   NDUFS8 (NDUFS8 Human)
   

   Use

   Tags

   myc-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. sgRNA BRCA1 C64Y

   Plasmid

   #139326

   Purpose

   Plasmid expressing a sgRNA to introduce BRCA1 C64Y using base editing

   Depositor

   Alberto Ciccia

   Article

   Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

   Insert

   sgRNA to insert BRCA1 C64Y using base editing
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pWZLblasti-HA-MEK1 C121S

   Plasmid

   #53165

   Purpose

   Expresses HA tagged human MEK1 C121S from blasticidin resistance retroviral vector

   Depositor

   Christopher Counter

   Article

   Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Insert

   MEK1 (MAP2K1 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   C121S

   Promoter

   Available since

   June 24, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pLVX neo ACTN1-GFP, Y246E

   Plasmid

   #138294

   Purpose

   Mammalian Expression, Lentiviral. Expression of human alpha-actinin-1-GFP, Y246E fusion protein

   Depositor

   Gaudenz Danuser

   Article

   Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

   Insert

   ACTN1 (ACTN1 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Y246E

   Promoter

   CMV

   Available since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-GABARα1N-HA

    Plasmid

    #128110

    Purpose

    Express GABARα1 N terminal domain

    Depositor

    Huda Zoghbi

    Article

    Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

    Insert

    Gabra1 (Gabra1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. sgRNA BRCA1 Q1779Stop

    Plasmid

    #139331

    Purpose

    Plasmid expressing a sgRNA to introduce BRCA1 Q1779Stop using base editing

    Depositor

    Alberto Ciccia

    Article

    Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

    Insert

    sgRNA to insert BRCA1 Q1779Stop using base editing
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pUC19-U6-BsmBI_cassette-CjeCas9-sgRNA (KAC482)

    Plasmid

    #133793

    Purpose

    U6 promoter sgRNA entry vector used for all CjeCas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to V2-TGAA linker sgRNA architecture from Kim et al. Nature Communications 2017

    Depositor

    Benjamin Kleinstiver, Joseph Bondy-Denomy

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    CjeCas9 sgRNA entry vector
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    V2-TGAA linker sgRNA architecture from Kim et al.…

    Promoter

    U6

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. sgRNA BRCA1 T922I

    Plasmid

    #139328

    Purpose

    Plasmid expressing a sgRNA to introduce BRCA1 T922I using base editing

    Depositor

    Alberto Ciccia

    Article

    Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

    Insert

    sgRNA to insert BRCA1 T922I using base editing
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDEST-FRT/T0-GFP-BRCA1 1364-1437

    Plasmid

    #71119

    Purpose

    Mammalian expression of GFP-BRCA1 1364-1437

    Depositor

    Daniel Durocher

    Article

    Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    contains amino acids 1364-1437 of BRCA1

    Promoter

    CMV/TO

    Available since

    Dec. 9, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pSN-A112C-GpA-G83I

    Plasmid

    #191239

    Purpose

    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of GpA-G83I and a His-tag, for fluorescent studies.

    Depositor

    Alessandro Senes

    Article

    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do

    Insert

    nuc (nuclease A), GYPA (GPA) (E(transmembrane)R)
    

    Use

    Tags

    nuclease A from S. aureus fused to the TM domain …

    Expression

    Bacterial

    Mutation

    Changed Alanine 112 to Cysteine in SN, and Glycin…

    Promoter

    T7 promoter

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGEX6P1-HA-MEK1 C121S

    Plasmid

    #53164

    Purpose

    Expresses GST-HA tagged human MEK1 C121S from bacterial expression vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    MEK1 (MAP2K1 Human)
    

    Use

    Tags

    GST and HA

    Expression

    Bacterial

    Mutation

    C121S

    Promoter

    Available since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pSUPER-retro-puro-tetO-Mek1-shRNA

    Plasmid

    #53179

    Purpose

    Expresses shRNA against mouse Mek1 from puromycin resistance retroviral vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    MEK1 (Map2k1 Mouse)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 19, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pGEX6P1-HA-MEK1 CBM

    Plasmid

    #53163

    Purpose

    Expresses GST-HA tagged human MEK1 M187AH188AM230AH239A from bacterial expression vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    MEK1 (MAP2K1 Human)
    

    Use

    Tags

    GST and HA

    Expression

    Bacterial

    Mutation

    M187A/H188A/M230A/H239A

    Promoter

    Available since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pGEX6P1-HA-MEK5 CBM-E

    Plasmid

    #53168

    Purpose

    Expresses GST-HA tagged human MEK5 L280AH281AM323AQ332A from bacterial expression vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    MEK5 (MAP2K5 Human)
    

    Use

    Tags

    GST and HA

    Expression

    Bacterial

    Mutation

    L280A/H281A/M323A/Q332A

    Promoter

    Available since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pET21a Sa-iPGM-bio

    Plasmid

    #179523

    Purpose

    Expresses S. aureus iPGM containing a C-terminal biotinylation site and His tag in E. coli

    Depositor

    James Inglese

    Article

    van Neer et al ACS Chem Biol. 2022 Aug 19;17(8):2284-22

    Insert

    S. aureus iPGM bio-6His
    

    Use

    Tags

    His tag and biotinylation sequence

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge