We narrowed to 6,084 results for: nls

1. AAV-Synapsin-miRFP670-3XNLS-WPRE

   Plasmid

   #159952

   Purpose

   nucleus marker

   Depositor

   Michael Courtney

   Article

   Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

   Insert

   miRFP670-3XNLS
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   synthetic

   Promoter

   hSyn1

   Available since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-T7-AcrIIA17_Efa-NLS(SV40) (KAC91)

   Plasmid

   #134333

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized AcrIIA17_Efa with C-terminal NLS (SV40)

   Depositor

   Joseph Bondy-Denomy, Benjamin Kleinstiver

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   human codon optimized AcrIIA17_Efa
   

   Use

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNTKV- Elf5-KI-3'UTR-2A-EYFP-NLS

   Plasmid

   #128833

   Purpose

   Knock-in of nuclear EYFP into mouse Elf5 locus

   Depositor

   Yossi Buganim

   Article

   Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

   Insert

   Nuclear EYFP into Elf5 locus (Elf5 Mouse)
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. 3xnls-cpmTq2-Calcium-lifetime-sensor

   Plasmid

   #129626

   Purpose

   Genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes

   Depositor

   Dorus Gadella

   Article

   van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

   Insert

   3xnls-cpmTq2-Calcium-lifetime-sensor
   

   Use

   Tags

   3xNLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pC4EN-F1-mGAF709 (NLS-FKBP-mGAF709)

   Plasmid

   #80004

   Purpose

   miRFP709 GAF domain fused to FKBP and NLS

   Depositor

   Vladislav Verkhusha

   Article

   Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

   Insert

   FKBP
   

   Use

   Tags

   GAF domain of miRFP709 and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCA15-Ubc-NLS- scFV-tdTomato

   Plasmid

   #199446

   Purpose

   Expression of scFV-tdTomato that binds to the GCN4 peptide from the SunTag System in mammalian cells

   Depositor

   Baohui Chen

   Article

   Xu et al J Virol. 2022 Dec 21;96(24):e0092022. do

   Insert

   scFV-tdTomato
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human ubiquitin C (UBC)

   Available since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAG413 GAL ORF1 Shuffle1 NLS

   Plasmid

   #145794

   Purpose

   mPing Yeast Transposition Assay

   Depositor

   Charles Nathan Hancock

   Article

   Payero et al Journal of the South Carolina Academy of

   Insert

   ORF1 Shuffle1 NLS
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Enhanced Nucleur Localization Signal

   Promoter

   Available since

   Jan. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEMHE-NLS-Beta-catenin-S33Y

   Plasmid

   #138537

   Purpose

   For making Beta-catenin-S33Y RNA

   Depositor

   Carmen Williams

   Article

   Gambini et al Dev Cell. 2020 Jun 8;53(5):545-560.e7. d

   Insert

   Beta-catenin
   

   Use

   In vitro transcription

   Tags

   2XFLAG and 2xNLS

   Expression

   Mutation

   changed serine 33 to tyrosine

   Promoter

   T7

   Available since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. FokI(inactive)-mCherry-LacI-NLS

   Plasmid

   #215100

   Purpose

   Expresses FokI(inactive)-mCherry-LacI-NLS in mammalian cells.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   FokI(inactive)-mCherry-LacI-NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   FokI(inactive) mutant D69A.

   Promoter

   CMV

   Available since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEJS1004-pCSDest2-AcrIIA5-NLS-FLAG

    Plasmid

    #136513

    Purpose

    Expresses type II-A anti-CRISPR protein AcrIIA5 in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Garcia et al Cell Rep. 2019 Nov 12;29(7):1739-1746.e5

    Insert

    codon-optimized AcrIIA5
    

    Use

    Tags

    FLAG/NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. EYFP-NLS-actin-K68/284R

    Plasmid

    #60616

    Purpose

    Encodes YFP and NLS tagged beta-actin with sumoylation resistant mutations

    Depositor

    Primal de Lanerolle

    Article

    Hofmann et al J Cell Biol. 2009 Jul 27;186(2):193-200.

    Insert

    Beta-actin (ACTB Human)
    

    Use

    Tags

    EYFP and NLS

    Expression

    Mammalian

    Mutation

    K68R, 284R

    Promoter

    Available since

    Nov. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pT2ADW-lox-mCherryNLS-pA-lox-4493NES

    Plasmid

    #138374

    Purpose

    Booster-PKA for the generation of mice

    Depositor

    Michiyuki Matsuda

    Article

    Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acss

    Insert

    lox-mCherry-lox-Booster-PKA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCSD_N-SpCas9-1-573-FRB-1xNLS

    Plasmid

    #107298

    Purpose

    Expresses N terminal SpCas9 (1-573 aa) fused to FRB in mammalian cells

    Depositor

    Scot Wolfe

    Insert

    Truncated SpCas9 (aa 1-573) fused to FRB
    

    Use

    CRISPR

    Tags

    FRB and SV40 NLS

    Expression

    Mammalian

    Mutation

    Truncated SpCas9 (aa 1-573)

    Promoter

    CMV IE94

    Available since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. A3Ai E72A-Cas9n-UGI-NLS

    Plasmid

    #109430

    Purpose

    Expresses catalytically inactive human APOBEC3A with an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.

    Depositor

    Reuben Harris

    Article

    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

    Insert

    Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3A E72A Catalytic Mutant (APOBEC3A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Insertion of an L1 intron into the coding sequenc…

    Promoter

    CMV

    Available since

    July 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pIRES-mRFP-NLS-PCNA (2514)

    Plasmid

    #61850

    Purpose

    cell cycle marker

    Depositor

    Jonathon Pines

    Insert

    PCNA (PCNA Human)
    

    Use

    Tags

    NLS and mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pCMV-T7-hAcrVA2.1-NLS(sv40) (BPK5059)

    Plasmid

    #115137

    Purpose

    CMV-T7 promoter expression plasmid for human codon optimized AcrVA2.1 anti-CRISPR protein with C-terminal NLS

    Depositor

    Keith Joung, Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Marino et al Science. 2018 Sep 6. pii: science.aau517

    Insert

    human codon optimized AcrVA2.1 anti-CRISPR protein
    

    Use

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHR: pPGK PYL1-sfGFP-NLS

    Plasmid

    #174116

    Purpose

    Expression of PYL1-sfGFP-NLS

    Depositor

    Stanley Qi

    Article

    Gao et al Mol Cell. 2021 Aug 13. pii: S1097-2765(2

    Insert

    PYL1-sfGFP-NLS
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    PGK

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pmyc-GFP-TNRC6A-NLS-mut

    Plasmid

    #42000

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    R930A, R931A, R932A, R934A

    Promoter

    CMV

    Available since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV 4xSTAR-mTFP-NLS-blast

    Plasmid

    #136257

    Purpose

    Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

    Depositor

    Hugo Snippert

    Article

    Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

    Insert

    stem cell Ascl2 reporter, 4 repeats
    

    Use

    Lentiviral

    Tags

    mTFP-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. Col2a1:TFPnls-mErt-Cre-Ert

    Plasmid

    #111152

    Purpose

    Chondrocyte specific inducible Cre

    Depositor

    Elly Tanaka

    Insert

    TFPnls-T2a-mErt-Cre-Ert
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    wt

    Promoter

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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