We narrowed to 2,190 results for: D10

1. pREDIT_Cas9n-MS2-BB_BbsI

   Plasmid

   #164804

   Purpose

   REDIT backbone for nickase Cas9n, pU6-MS2-gRNA-backbone(BbsI)-CBH-SpCas9n(D10A)-T2A-EGFP

   Depositor

   Le Cong

   Article

   Wang et al Nucleic Acids Res. 2021 Apr 6;49(6):e36.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRK5-mFzd2

   Plasmid

   #42254

   Depositor

   Jeremy Nathans, Chris Garcia

   Article

   Yu et al Development. 2012 Dec 1;139(23):4383-94.

   Insert

   Frizzled 2 (Fzd2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. CREB3L3

   Plasmid

   #99509

   Purpose

   to express CREB3L3 in mammalian system

   Depositor

   Dong-Yan Jin

   Article

   Chin et al Nucleic Acids Res. 2005 Mar 30;33(6):185

   Insert

   CREB3L3 (Creb3l3 Mouse)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRS315e_pGal-dCas9-PmCDA1

   Plasmid

   #79615

   Purpose

   Expresses dCas9-PmCDA1 in yeast cells

   Depositor

   Akihiko Kondo

   Article

   Nishida et al Science. 2016 Aug 4. pii: aaf8729.

   Insert

   SpCas9
   

   Use

   Tags

   PmCDA1

   Expression

   Yeast

   Mutation

   D10A and H840A for nuclease deficient Cas9

   Promoter

   pGal1

   Available since

   July 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. dCBE4-gam

   Plasmid

   #124449

   Purpose

   Expresses dead Cas9 (dCas9)-CBE4-gam

   Depositor

   George Church

   Article

   Smith et al Nucleic Acids Res. 2020 May 21;48(9):518

   Insert

   dCBE4-gam
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   Available since

   April 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. NAX_CAGGS_CAS9_m4_VPR_mcherry

   Plasmid

   #167886

   Purpose

   PiggyBac compatible plasmid expressing dCas9-VPR

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-VPR
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, D839A, H840A, N863A

   Promoter

   Available since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

7. pJMP1333

   Plasmid

   #119268

   Purpose

   rfp "test" strain

   Depositor

   Oren Rosenberg, Jason Peters, Carol Gross

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   sgRNA NT1/RR1 (rfp)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D10A and H840A

   Promoter

   Available since

   Jan. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. 3xFLAG-dCas9/pMSCVpuro

   Plasmid

   #134983

   Purpose

   Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

   Depositor

   Hodaka Fujii

   Article

   Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa

   Insert

   3xFLAG-dCas9
   

   Use

   CRISPR and Retroviral

   Tags

   3xFLAG tag and NLS

   Expression

   Mammalian

   Mutation

   human codon-optimized, D10A + H840A

   Promoter

   LTR

   Available since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLicC-MBP-APETx2

   Plasmid

   #72668

   Purpose

   Periplasmic expression of APETx2 in E.coli

   Depositor

   Glenn King

   Article

   Anangi et al Mar Drugs. 2012 Jul;10(7):1605-18. doi:

   Insert

   APETx2
   

   Use

   Tags

   His tag, MBP, MalE signal sequence for periplasmi…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJMP1187

    Plasmid

    #119256

    Purpose

    rfp "test" strain

    Depositor

    Oren Rosenberg, Jason Peters, Carol Gross

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    sgRNA NT1/RR1 (rfp)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. EE-BE4max

    Plasmid

    #138158

    Purpose

    C-to-T base editor

    Depositor

    David Liu

    Article

    Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

    Insert

    rAPOBEC1(EE)-nSpCas9-UGI-UGI
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Within rAPOBEC1 R126E + R132E; within Cas9 D10A

    Promoter

    CMV

    Available since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pENTR1A hu_ncOGT WT

    Plasmid

    #154281

    Purpose

    Entry vector encoding non-tagged O-GlcNAc Transferase

    Depositor

    Suzanne Walker

    Article

    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Gateway entry vector

    Tags

    2x FLAG and mKate2

    Expression

    Mutation

    WT OGT

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. mEos3.2trCD45

    Plasmid

    #203753

    Purpose

    Encodes the transmembrane domain from CD45 with mEos3.2 fused on the N terminus. Used as a fluorescent plasma membrane probe.

    Depositor

    Sarah Veatch

    Article

    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

    Insert

    trCD45 (PTPRC Human)
    

    Use

    Tags

    mEos3.2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJMP1360

    Plasmid

    #119278

    Purpose

    for cloning new sgRNAs

    Depositor

    Oren Rosenberg, Jason Peters, Carol Gross

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    dcas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJMP1189

    Plasmid

    #119257

    Purpose

    rfp "test" strain

    Depositor

    Oren Rosenberg, Jason Peters, Carol Gross

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    dcas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. CD45 sp-SNAPf-CD45R0Ex-His10 (XSB316)

    Plasmid

    #78509

    Purpose

    Construct for expressing CD45R0 (extracellular domain) His10 in Sf9

    Depositor

    Ron Vale

    Article

    Su et al Science. 2016 Apr 29;352(6285):595-9. do

    Insert

    CD45R0Ex (PTPRC Human)
    

    Use

    Tags

    His10 and SNAPf

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. HiFi dCas9 KRAB

    Plasmid

    #188500

    Purpose

    Expresses FLAG tagged HiFi dCas9 KRAB

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A/R691A/H840A

    Promoter

    EF1a

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJMP1223

    Plasmid

    #119261

    Purpose

    rfp "test" strain

    Depositor

    Oren Rosenberg, Jason Peters, Carol Gross

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    dcas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJMP1221

    Plasmid

    #119260

    Purpose

    rfp "test" strain

    Depositor

    Oren Rosenberg, Jason Peters, Carol Gross

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    sgRNA NT1/RR1 (rfp)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJMP1335

    Plasmid

    #119269

    Purpose

    rfp "test" strain

    Depositor

    Oren Rosenberg, Jason Peters, Carol Gross

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    dcas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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