We narrowed to 5,868 results for: nls

1. pZLCv2-gMYOD1-3xFLAG-dCas9-HA-2xNLS

   Plasmid

   #106355

   Purpose

   CRISPR/Cas9 engineered chromatin immunoprecipitation of MYOD1 locus

   Depositor

   Stephen Tapscott

   Article

   Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

   Insert

   MYOD1 gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMT-zic2a-NLS-BirA-2A-mCherry_Ras

   Plasmid

   #80065

   Purpose

   Zic2a promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
   

   Use

   Zebrafish biotagging

   Tags

   3x HA

   Expression

   Bacterial

   Mutation

   Promoter

   Zic2a enhancer driving c-fos promoter

   Available since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJFRC7-RFPnls-PQRv2-mCD8:GFP

   Plasmid

   #73974

   Purpose

   Protein Quantitation Reporter (PQR) to quantify a protein of interest in insect cells.

   Depositor

   Brian Chen

   Article

   Lo et al Cell Rep. 2015 Dec 22;13(11):2634-44. do

   Inserts

   sfGFP
   RFP
   

   Use

   Tags

   mCD8 and nls

   Expression

   Insect

   Mutation

   Promoter

   20XUAS-HS

   Available since

   July 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. p3xFLAG-CMV-Drosha (NLS-P426)

   Plasmid

   #171538

   Purpose

   Encodes mutant human Drosha protein

   Depositor

   Yan Zeng

   Article

   Zhang et al Biochim Biophys Acta Gene Regul Mech. 20

   Insert

   DROSHA (DROSHA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   an SV40 NLS sequence (PKKKRKVGI) insertion into t…

   Promoter

   Available since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMT-myl7-NLS-BirA-2A-mCherry_Ras

   Plasmid

   #80061

   Purpose

   Myl7 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
   

   Use

   Zebrafish biotagging

   Tags

   3x HA

   Expression

   Bacterial

   Mutation

   Promoter

   Myl7 promoter

   Available since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-T7-AcrIIA17_Sga-NLS(SV40) (KAC92)

   Plasmid

   #134334

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized AcrIIA17_Sga with C-terminal NLS (SV40)

   Depositor

   Joseph Bondy-Denomy, Benjamin Kleinstiver

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   human codon optimized AcrIIA17_Sga
   

   Use

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-T7-AcrIIA18_Sma-NLS(SV40) (KAC95)

   Plasmid

   #134335

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized AcrIIA18_Sma with C-terminal NLS (SV40)

   Depositor

   Joseph Bondy-Denomy, Benjamin Kleinstiver

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   human codon optimized AcrIIA18_Sma
   

   Use

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-T7-AcrIIA19_Ssim-NLS(SV40) (KAC97)

   Plasmid

   #134336

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized AcrIIA19_Ssim with C-terminal NLS (SV40)

   Depositor

   Joseph Bondy-Denomy, Benjamin Kleinstiver

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   human codon optimized AcrIIA19_Ssim
   

   Use

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-T7-AcrIIA18_Sga-NLS(SV40) (KAC214)

   Plasmid

   #134338

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized AcrIIA18_Sga with C-terminal NLS (SV40)

   Depositor

   Joseph Bondy-Denomy, Benjamin Kleinstiver

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   human codon optimized AcrIIA18_Sga
   

   Use

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-T7-AcrIIA19_Spse-NLS(SV40) (KAC215)

    Plasmid

    #134339

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AcrIIA19_Spse with C-terminal NLS (SV40)

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized AcrIIA19_Spse
    

    Use

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pZLCv2-gD4Z4-2-3xFLAG-dCas9-HA-2xNLS

    Plasmid

    #106353

    Purpose

    CRISPR/Cas9 engineered chromatin immunoprecipitation of D4Z4 locus

    Depositor

    Stephen Tapscott

    Article

    Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

    Insert

    D4Z4 gRNA-2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pINDUCER20-Flag-NLS-hSPDEF delta1

    Plasmid

    #100874

    Purpose

    Lentiviral expression of human SPDEF truncation

    Depositor

    Noah Shroyer

    Article

    Lo et al Gastroenterology. 2017 Jul;153(1):205-21

    Insert

    SPDEF (SPDEF Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    truncated protein 1-248aa

    Promoter

    TRE2

    Available since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. SPL10p::pCGTAG (SPL10p::NLS-GFP)

    Plasmid

    #27410

    Depositor

    David Bartel

    Article

    Nodine et al Genes Dev. 2010 Dec 1. 24(23):2678-92.

    Insert

    SPL10 promoter (AT1G27370 Mustard Weed)
    

    Use

    Tags

    Expression

    Mutation

    SPL10 promoter transcriptionally fused to a nucle…

    Promoter

    Available since

    Feb. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEJS583-pCSDest2-AcrIIC5Smu-FLAG-NLS

    Plasmid

    #113437

    Purpose

    Expresses a FLAG/NLS tagged type II-C anti-CRISPR protein from S. muelleri in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

    Insert

    Type II-C anti-CRISPR
    

    Use

    CRISPR

    Tags

    FLAG/NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSLIK 3XFLAG-MKK7-EE-NLS hygro

    Plasmid

    #87780

    Purpose

    Inducible expression of constitutively active mutant MKK7 with a C-terminal NLS sequence

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    MKK7 (Map2k7 Mouse)
    

    Use

    Lentiviral

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

16. pAAVf-EnhCB-lacZnls miR-122 3x

    Plasmid

    #35644

    Depositor

    Phillip Zamore

    Article

    Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

    Insert

    3 miR-122 target sites
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CB

    Available since

    April 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHAGE-EFS-Halo-NLS-tDeg

    Plasmid

    #236370

    Purpose

    Overexpression of Halo-NLS-tDeg in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

    Insert

    Halo-NLS-tDeg
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV.CAG-NLS-EGFP-WPRE-SV40pA-T7-T3-BC(p1-14)

    Plasmid

    #231345

    Purpose

    Single stranded AAV genome with components for tracking AAV genome via AAV-Zombie. Also expresses a nuclear localized EGFP from CAG promoter.

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    T7/T3-BC(p1-14)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. p2T-CMV-EA-evoAPOBEC1max-32NLS_NG-BlastR

    Plasmid

    #232722

    Purpose

    C-to-T base editor

    Depositor

    David Liu

    Article

    Matuszek et al Nat Genet. 2025 May 26. doi: 10.1038/s41

    Insert

    evoAPOBEC1max H47E+S48A-32NLS linker-nCas9(NG)-UGI-UGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    within evoAPOBEC1: H47E+S48A; within Cas9 D10A

    Promoter

    Available since

    March 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAGEN-Dre-nls (JDW 1131)

    Plasmid

    #229809

    Purpose

    A CAGGS-driven dre recombinase followed by a c-terminal 1x nls sequence

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    DRE recombinase-nls
    

    Use

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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