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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 2,114 results for: AP1;

Showing: 1891 - 1920 of 2114 results

  8. pIA33

    Plasmid
    #120803
    Purpose
    E. coli-C. difficile shuttle vector for CRISPR interference (CRISPRi) in C. difficile; sgRNA targets rfp; Pxyl::dCas9-opt Pgdh::sgRNA-rfp
    Depositor
    David S. Weiss, Craig Ellermeier
    Article
    Muh et al J Bacteriol. 2019 Feb 11. pii: JB.00711-
    Inserts
    deactivated nuclease dCas9, codon-optimized for C. difficile
    single guide RNA targeting red fluorescent protein
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Base-pairing region targets red fluorescent prote…
    Promoter
    Pgdh and Pxyl
    Available since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    120803-plasmid-map-sequence-id-235316

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  9. pBetaActin-FKBP-mScarlet-Dync1h1motor

    Plasmid
    #191330
    Purpose
    Expresses the fluorescently labeled dynein motor domain fused to dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB
    Depositor
    Frank Bradke
    Article
    Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:
    Insert
    FKBP-mScarlet-Dync1h1motor (Dync1h1 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    DYNC1H1 motor domain aa 1453-4644
    Promoter
    Available since
    Jan. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    191330-plasmid-map-sequence-id-381732

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  14. pGEX:PGRMC1

    Plasmid
    #136062
    Purpose
    Bacterial expression of pGEX:PGRMC1 to perform a GST-pulldown assay and identify new proteins that interact with GST-PGRMC1 in the human endometrial stroll cells.
    Depositor
    Francisco Domínguez Hernández
    Article
    Salsano et al Fertil Steril. 2020 May;113(5):1050-1066
    Insert
    Progesterone receptor membrane component 1 (PGRMC1 Human)
    Use
    Tags
    GST-PGRMC1
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    136062-plasmid-map-sequence-id-272028

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  18. TFORF3445

    Plasmid
    #144921
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    PTTG1 (PTTG1 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144921-plasmid-map-sequence-id-404503

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  20. TFORF3165

    Plasmid
    #144641
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TRIM28 (TRIM28 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    144641-plasmid-map-sequence-id-348484

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  21. pCDNA3-EMTB-TurboID-V5

    Plasmid
    #190736
    Purpose
    Mammalian expression of EMTB fusion to V5-tagged TurboID on microtubules (EMTB as the microtubule-targeting tag)
    Depositor
    Jeremy Baskin
    Article
    Cao et al ACS Chem Biol. 2022 Aug 11. doi: 10.1021
    Insert
    ensconsin (MAP7 Human)
    Use
    Tags
    Myc, TurboID, and V5
    Expression
    Mammalian
    Mutation
    Microtubule binding-domain of ensconsin (amino ac…
    Promoter
    CMV
    Available since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    190736-plasmid-map-sequence-id-379320

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  26. pENT-L1-Kozak-Ensconsin-3XGFP-stop-L2

    Plasmid
    #186361
    Purpose
    Entry clone with ORF encoding the MT-binding domain of Ensconsin fused to 3 copies of GFP in C-terminal, flanked by Gateway recombination sequences.
    Depositor
    Patrick Lemaire
    Article
    Roure et al PLoS One. 2007. 2(9):e916.
    Insert
    Human Ensconsin (MAP7 Synthetic, Human)
    Use
    Expression of a fluorescent microtubule marker
    Tags
    3xEGFP
    Expression
    Mutation
    Promoter
    Available since
    Nov. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    186361-plasmid-map-sequence-id-373431

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  29. LentiGuide-Puro-NCOA7g1 (BB22)

    Plasmid
    #139457
    Purpose
    Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable marker
    Depositor
    Caroline Goujon
    Article
    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.
    Insert
    NCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR (NCOA7 Synthetic)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a / U6
    Available since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    139457-plasmid-map-sequence-id-308472

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  30. LentiGuide-Puro-NCOA7g2 (BB23)

    Plasmid
    #139458
    Purpose
    Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable marker
    Depositor
    Caroline Goujon
    Article
    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.
    Insert
    NCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR (NCOA7 Synthetic)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a / U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    139458-plasmid-map-sequence-id-308461

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Showing: 1891 - 1920 of 2114 results