We narrowed to 17,223 results for: Emb;
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Plasmid#233256PurposeExpression of BFP-tagged ARF6 WT for Turbo ID experimentsDepositorInsertARF6 (ARF6 Human)
UseLentiviralAvailable SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsAGO2-K663AL697Y_AC
Plasmid#148427PurposeMammalian Expression of HsAGO2-K663AL697YDepositorInsertHsAGO2-K663AL697Y (AGO2 Human)
ExpressionMammalianMutationthree silent mutations and the sequence differs i…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsAGO2-P593GA623FK663AL697Y_AC
Plasmid#148428PurposeMammalian Expression of HsAGO2-P593GA623FK663AL697YDepositorInsertHsAGO2-P593GA623FK663AL697Y (AGO2 Human)
ExpressionMammalianMutationthree silent mutations and the sequence differs i…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-DmPABPC1-del183-258-siRNAres-V5His6_L
Plasmid#146862PurposeInsect Expression of DmPABPC1_del183-258-siRNAresDepositorInsertDmPABPC1_del183-258-siRNAres (pAbp Fly)
ExpressionInsectMutationone non silent mutation G26S, and seven silent mu…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-DmPABPC1-del288-359-siRNAres-V5His6_L
Plasmid#146863PurposeInsect Expression of DmPABPC1_del288-359-siRNAresDepositorInsertDmPABPC1_del288-359-siRNAres (pAbp Fly)
ExpressionInsectMutationone non silent mutation G26S, and six silent muta…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-DmPABPC1-del364-550-siRNAres-V5His6_L
Plasmid#146864PurposeInsect Expression of DmPABPC1_del364-550-siRNAresDepositorInsertDmPABPC1_del364-550-siRNAres (pAbp Fly)
ExpressionInsectMutationone non silent mutation G26S, and five silent mut…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
W118-1_hZBTB7B_R363L
Plasmid#180381Purposelentiviral transduction of human ZBTB7B gene with a point mutation (R363L) in the first ZF domainDepositorAvailable SinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
W118-1_hZBTB7B_D35N_R49Q
Plasmid#180383Purposelentiviral transduction of human ZBTB7B gene with point mutations (D35N and R49Q) in the BTB domainDepositorInsertZBTB7B_D35N/R49Q (ZBTB7B Human)
UseLentiviralExpressionMammalianMutationD35N/R49QPromoterCMVAvailable SinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
W118-1_hZBTB7B_R49H
Plasmid#180382Purposelentiviral transduction of human ZBTB7B gene with a point mutation (R49H) in the BTB domainDepositorAvailable SinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-TAPtagN-PrP-SBP-HsAGO2_AC
Plasmid#148440PurposeMammalian Expression of HsAGO2DepositorInsertHsAGO2 (AGO2 Human)
ExpressionMammalianMutationone silent mutation C840Tand sequence differs in …Available SinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET15b MOSPD2 [315-445]
Plasmid#186474PurposeExpression of human MOSPD2 (MSP domain from residue 315 to 445), fused to a cleavable 6HIS tag, in bacteriaDepositorInsertMOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
Tags6HISExpressionBacterialMutationMSP domain [315-445]Available SinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-EGFP-eDHFR(69K6)-cRaf
Plasmid#178849PurposeMammalian expression of cRaf fused to EGFP-eDHFR(69K6)DepositorAvailable SinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-cRaf-mNG-eDHFR(69K6)
Plasmid#172107PurposeMammalian expression of cRaf fused to mNeonGreen-eDHFR(69K6)DepositorAvailable SinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJET-22-DExCon-mNeonGreen-R11a
Plasmid#179897PurposeDonor with homologous arms for Rab11a to knock in DExCon-mNeonGreen moduleDepositorInsertRAB11A, member RAS oncogene family (RAB11A Human)
UseCRISPR; Donor for homologous based knock inTagsmNeonGreenExpressionBacterial and MammalianPromoterTRE3GSAvailable SinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsRck_296-472-10xmut_AC
Plasmid#148420PurposeMammalian Expression of HsRck_296-472-10xmutDepositorInsertHsRck_296-472-10xmut (DDX6 Human)
ExpressionMammalianMutationQ309A, H312A, T316A, R320A, Q322A, N324A, R335A, …Available SinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV.CBA.YFP.miR-E_shRps6ka2-3
Plasmid#180390PurposeProducing AAV that encodes mouse Rps6ka2 (Rsk3) shRNA-3 with miR-E backboneDepositorAvailable SinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV.CBA.YFP.miR-E_shRps6ka2-2
Plasmid#180389PurposeProducing AAV that encodes mouse Rps6ka2 (Rsk3) shRNA-2 with miR-E backboneDepositorAvailable SinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV.CBA.YFP.miR-E_shRps6ka2-1
Plasmid#180388PurposeProducing AAV that encodes mouse Rps6ka2 (Rsk3) shRNA-1 with miR-E backboneDepositorAvailable SinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-kappa-myc-dK-2xG4S-TMst
Plasmid#145766PurposeExpresses myc-dL5(E52K, L91S) low affinity Fluorogen Activating Protein (FAP) on the extracellular surface anchored to the mammalian cell plasma membrane with the PDGFR transmembrane domain.DepositorInsertkappa-myc-dK-2xG4S-TMst
ExpressionMammalianMutationThe dK FAP is dL5 with mutations at E50K, L89S, a…PromoterCMVAvailable SinceJan. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-mAmber
Plasmid#105792PurposeExpression of your protein of interest in fusion with non-absorbing, non-emitting Venus mutant at the C-terminus (cleavable by TEV). Negative control for FRET measurements (PMID: 17040988, 22264545).DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-mAmberExpressionMammalianAvailable SinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKK-mAmber-TEV
Plasmid#105775PurposeExpression of your protein of interest in fusion with non-absorbing, non-emitting Venus mutant at the N-terminus (cleavable by TEV). Negative control for FRET measurements (PMID: 17040988, 22264545).DepositorTypeEmpty backboneUseFlp-in competentTagsmAmber-TEVExpressionMammalianAvailable SinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
10xHis-ataxin-3 *SIM-HA
Plasmid#89983PurposeExpresses His- and HA-tagged ataxin-3 with a mutation in the SUMO-interacting motifDepositorInsertataxin-3 (ATXN3 Human)
Tags10xHis and HAExpressionMammalianMutationmutated aa 162IFVV to 162AFAAAvailable SinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
GFP-ataxin-3 *SIM
Plasmid#89979PurposeExpresses GFP-tagged ataxin-3 with a mutation in the SUMO-interacting motifDepositorAvailable SinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
NKP9 pmRFP-N2 (719)
Plasmid#61999Purposemammalian expression of nuclear envelope transmembrane proteinDepositorAvailable SinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
NKP40 RFP (794)
Plasmid#62006Purposemammalian expression of nuclear envelope transmembrane proteinDepositorAvailable SinceJan. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTH731-2µ-RLuc/minCFLuc
Plasmid#40601DepositorInsertsFirefly Luciferase
Renilla luciferase
ExpressionYeastMutationAll codons of the original FLuc gene were exchang…PromoterADH1 and TDH3Available SinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
STT3A pmRFP (663)
Plasmid#61997Purposemammalian expression of nuclear envelope transmembrane proteinDepositorAvailable SinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKK-BI16-ORF1-3C-mRuby3_ORF2-TEV-mClover3
Plasmid#105802PurposeConcomitant expression of two proteins. One protein expressed with mRuby3, cleavable by 3C; second protein expressed with mClover3, cleavable by TEV protease; tags positions: C-termini.DepositorTypeEmpty backboneUseFlp-in competentTagsORF1: 3C-mRuby3; ORF2: TEV-mClover3ExpressionMammalianAvailable SinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV Jph3gRNA mEmerald hSyn mTagBFP2-CAAX2
Plasmid#236246PurposeEndogenous tagging of Junctophilin 3 with mEmerald with a fluorescent marker for the plasma membraneDepositorInsertgRNA for rat Jph3, mEmerald donor and mTagBFP2-CAAX2
UseAAVTagsmTagBFP2PromoterU6 and human Synapsin1Available SinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-POMP-PSMG1-PSMG2-PSMG3-PSMG4
Plasmid#214137PurposeInsect cell expression of the human 20SCP proteasome assembly chaperonesDepositorInsertsExpressionInsectPromoterpolyhedrinAvailable SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN-Vhh-LaminB-mNeonGreen-IRES-myr-BFP (JDW 1353)
Plasmid#229845PurposeA CAGGS-driven expression vector containing a vhh-Lamin nanobody for nuclear lamina labeling followed by an IRES and a myristoylated TagBFP for labeling the cell membrane.DepositorInsertLamin nanobody
ExpressionMammalianAvailable SinceFeb. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGM238
Plasmid#220178PurposeExpresses exogenous ribosomal-binding domain mutant NAC complex members (K78E NACA and K43E BTF3)DepositorUseLentiviralTags3xFLAG (NACA); 6xHis (BTF3)MutationK78E (NACA) + K43B (BTF3)Available SinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
STT3A pEGFP-N2 (1051)
Plasmid#62025Purposemammalian expression of nuclear envelope transmembrane proteinDepositorAvailable SinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pICE-HA-Ku70-siR-Mut6E
Plasmid#82330PurposePlasmid for constitutive or doxy-inducible expression of mutant human Ku70 unable to bind DNA and resistant to siRNA. Confers resistance to puro. Use T-REx cells for doxycycline-inducible expression.DepositorInsertKu70 (XRCC6 Human)
TagsHAExpressionMammalianMutationMut6E corresponding to K282E K287E T300E K331E K3…PromoterCMV-tetAvailable SinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKK-RNAi-nucEGFPmiR-TEV-mCherry
Plasmid#105812PurposeFunctional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear EGFP expression marker; your protein of interest with C-terminal mCherry.DepositorTypeEmpty backboneUseRNAi; Flp-in competentTagsTEV-mCherryExpressionMammalianAvailable SinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
Cherry-HA-KCC2
Plasmid#104077PurposeTo visualize the expression and localization of potassium-chloride cotransporter type 2 (KCC2)DepositorInsertKCC2 (b isoform) (Slc12a5 Rat)
TagsmCherry-HA tagExpressionMammalianMutationEcoR1 site in rat KCC2 was removed by silent muta…PromoterUbCAvailable SinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKK-RNAi-nucCHERRYmiR-EGFP-TEV
Plasmid#105810PurposeFunctional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with N-terminal EGFP.DepositorTypeEmpty backboneUseRNAi; Flp-in competentTagsEGFP-TEVExpressionMammalianAvailable SinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKK-RNAi-nucCHERRYmiR-FLAG-TEV
Plasmid#105809PurposeFunctional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with N-terminal FLAG.DepositorTypeEmpty backboneUseRNAi; Flp-in competentTagsFLAG-TEVExpressionMammalianAvailable SinceFeb. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKK-RNAi-nucEGFPmiR-TEV-FLAG
Plasmid#105811PurposeFunctional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear EGFP expression marker; your protein of interest with C-terminal FLAG.DepositorTypeEmpty backboneUseRNAi; Flp-in competentTagsTEV-FLAGExpressionMammalianAvailable SinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTH734-2µ-RLuc/stopCFLuc
Plasmid#40609DepositorInsertsFirefly Luciferase (nonsense mutant)
Renilla luciferase
ExpressionYeastMutationAll codons of the original FLuc gene were exchang…PromoterADH1 and TDH3Available SinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pKK-RNAi-nucEGFPmiR-mCherry-TEV
Plasmid#105808PurposeFunctional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear EGFP expression marker; your protein of interest with N-terminal mCherry.DepositorTypeEmpty backboneUseRNAi; Flp-in competentTagsmCherry-TEVExpressionMammalianAvailable SinceMay 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKK-RNAi-nucEGFPmiR-FLAG-TEV
Plasmid#105807PurposeFunctional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear EGFP expression marker; your protein of interest with N-terminal FLAG tag.DepositorTypeEmpty backboneUseRNAi; Flp-in competentTagsFLAG-TEVExpressionMammalianAvailable SinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
eIF3e- pMK289 (mAID-mClover-NeoR) plasmid
Plasmid#192239Purposeknockin donor vector of mAID-mClover-NeoR to C-terminus of endogenous human eIF3eDepositorInserteIF3e-knockin-homology arm-mAID-mClover-NeoR (EIF3E Human)
ExpressionMammalianAvailable SinceJan. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
eIF3f- pMK293 (mAID-mCherry2-Hygro) plasmid
Plasmid#192242Purposeknockin donor vector of mAID-mCherry2-Hygro to C-terminus of endogenous human eIF3fDepositorInserteIF3f-knockin-homology arm-mAID-mCherry2-Hygro (EIF3F Human)
ExpressionMammalianAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKK-RNAi-nucCHERRYmiR-TEV-FLAG
Plasmid#105813PurposeFunctional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with C-terminal FLAG.DepositorTypeEmpty backboneUseRNAi; Flp-in competentTagsTEV-FLAGExpressionMammalianAvailable SinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
W118-1_hRSK3(RPS6KA2)_K91A_K444A
Plasmid#180385Purposelentiviral transduction of human RSK3 (RPS6KA2) gene with point mutations (K91A and K444A) that inactivate kinase activityDepositorInsertRPS6KA2_K91A/K444A (RPS6KA2 Human)
UseLentiviralExpressionMammalianMutationK91A/K444APromoterCMVAvailable SinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmeIF4E
Plasmid#79258PurposeExpresses EGFP-tagged DmeIF4E in S2 cellsDepositorAvailable SinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
NET23 pEGFP-N2 (1174)
Plasmid#62037Purposemammalian expression of nuclear envelope transmembrane proteinDepositorAvailable SinceAug. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKK-RNAi-nucCHERRYmiR-TEV-EGFP
Plasmid#105814PurposeFunctional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with C-terminal EGFP.DepositorTypeEmpty backboneUseRNAi; Flp-in competentTagsTEV-EGFPExpressionMammalianAvailable SinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTH752-CEN-RLuc/min103maxCFLuc
Plasmid#38218DepositorInsertsFirefly Luciferase
Renilla luciferase
ExpressionYeastMutationThe first 103 codons of the original FLuc gene we…PromoterADH1 and TDH3 (=GPD)Available SinceOct. 22, 2012AvailabilityAcademic Institutions and Nonprofits only