We narrowed to 3,154 results for: FRI

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pBR322-Pdp1_NTD-ZFD_L

Plasmid

#198276

Purpose

For loading L half of zinc finger deaminase targeting human TRAC locus (ZFD_TRAC-NC-Left-G1397-N_DddA); also contains PVC regulatory genes

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

Pdp1_NTD-ZFD_L

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA FLAG TORC3

Plasmid

#22976

Depositor

Marc Montminy

Article

Screaton et al Cell. 2004 Oct 1. 119(1):61-74.

Insert

TORC3 (CRTC3 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
EF.deltaBHES1.Ubc.GFP

Plasmid

#24982

Depositor

Linzhao Cheng

Article

Yu et al Stem Cells. 2006 Apr . 24(4):876-88.

Insert

HES1 (HES1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

DNA-binding domain disrupted by E43A, K44A, R47A …

Available Since

June 14, 2010

Availability

Academic Institutions and Nonprofits only
p3E-U6a-U6c-ndufs2 guide

Plasmid

#107601

Purpose

An entry vector with U6a and U6c promoter driving ndufs2 guide RNAs expression

Depositor

Qing Deng

Article

Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

Insert

ndufs2 guide RNA sequences

Use

CRISPR

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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FLAG-Ago1

Plasmid

#21533

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

Ago1 (AGO1 Human)

Tags

FLAG

Expression

Mammalian

Available Since

July 13, 2009

Availability

Academic Institutions and Nonprofits only
OVA-miSFIT-17-T

Plasmid

#124677

Purpose

Tuning expression of cytosolic Ovalbumin coupled to EGFP

Depositor

Tudor Fulga

Article

Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

Insert

Cyto-Ovalbumin

Expression

Mammalian

Mutation

Truncated OVA for cytoplasmic expression

Promoter

minimal CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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Opto-casp9_VfAU1-LOV_casp9

Plasmid

#131220

Purpose

Expresses Opto-casp9_VfAU1-LOV_casp9 (light-activated caspase9)

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

Opto-casp9 (VfAU1-LOV_casp9)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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p3E-U6a-U6c-rac2 guide

Plasmid

#107600

Purpose

An entry vector with U6a and U6c promoter driving rac2 guide RNAs expression

Depositor

Qing Deng

Article

Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

Insert

rac2 guide RNA sequences

Use

CRISPR

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pCS2-sod1

Plasmid

#107594

Purpose

Contains zebrafish sod1 gene in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.

Depositor

Qing Deng

Article

Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

Insert

sod1 (sod1 Zebrafish)

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pMAL_dstβL

Plasmid

#73805

Purpose

Plasmid for selection for insertion and homodimerization

Depositor

Sarel Fleishman

Article

Elazar et al Elife. 2016 Jan 29;5. pii: e12125. doi:

Inserts

CAT Marker
ToxR
beta lactamase
spectomycin resistance
CLS TM domain

Tags

Part Of the dstbl chiemra, Part of the dsTbL chim…

Expression

Bacterial

Promoter

NaN and ToxR promotor

Available Since

March 14, 2016

Availability

Academic Institutions and Nonprofits only
pBR322-Pdp1_NTD-ZFD_R

Plasmid

#198277

Purpose

For loading R half of zinc finger deaminase targeting human TRAC locus (ZFD_TRAC-NC-Right-G1397-C_DddA); also contains PVC regulatory genes

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

Pdp1_NTD-ZFD_R

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pRS315-ADH700p-yeCherry-p150-yeGFP-CYCt

Plasmid

#194519

Purpose

Dual fluorescence reporter for studying protein degradation

Depositor

Tommer Ravid

Article

Mashahreh et al Nat Commun. 2022 Dec 8;13(1):7588. doi:

Insert

TIF4631 leader sequence

Expression

Yeast

Available Since

Feb. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSynapsin1-axon-GCaMP6m

Plasmid

#111261

Purpose

For enriched expression of GCaMP6m in axons

Depositor

Lin Tian

Article

Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

Has Service

AAV9

Insert

axon-GCaMP6m

Use

AAV

Tags

GAP43 palmitoylation domain

Expression

Mammalian

Promoter

hSynapsin1

Available Since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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pGI3EM09

Plasmid

#135482

Purpose

Agrobacterium T-DNA with SpunHsp70pr driving hph-GFP

Depositor

Nicolas Buchler

Article

Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

Insert

SpunHsp70pr hph-GFP ADH1t

Tags

GFP

Expression

Bacterial and Yeast

Promoter

SpunHsp70pr

Available Since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-GW182delta1

Plasmid

#11592

Depositor

Edward Chan

Article

Jakymiw et al Nat Cell Biol. 2005 Dec . 7(12):1267-74.

Insert

GW182 (TNRC6A Human)

Tags

GFP

Expression

Mammalian

Mutation

contains only aa1-498; SEE DEPOSITOR NOTES BELOW

Available Since

June 30, 2006

Availability

Academic Institutions and Nonprofits only
pEM09

Plasmid

#135479

Purpose

Spizellomyces Hsp70 promoter driving expression of Hygromycin resistance fused to GFP

Depositor

Nicolas Buchler

Article

Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

Insert

SpunHsp70pr

Tags

GFP and hph

Expression

Bacterial and Yeast

Promoter

Spun Hsp70pr

Available Since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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pRC1765

Plasmid

#141346

Purpose

Mini plasmid containing minimal sequences for amplification in bacteria and two BbvCI nicking sites for introduction of modified DNA through annealing and ligation (e.g. mismatch, DNA lesions, flap).

Depositor

Rafael Fernandez-Leiro

Article

Fernandez-Leiro et al Nat Struct Mol Biol. 2021 Apr;28(4):373-

Type

Empty backbone

Expression

Bacterial

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28-sfGFP-DogTag Loop B

Plasmid

#171780

Purpose

Expresses in bacterial cytoplasm superfolder GFP with DogTag and flanking linkers between Asp102 and Asp103

Depositor

Mark Howarth

Article

Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

Insert

sfGFP-DogTag Loop B

Tags

His6

Expression

Bacterial

Mutation

DogTag and linkers inserted between residues Asp1…

Promoter

T7

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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CMVp-ECFP-Triplex-28-8xmiRNA-BS-28-pA (Construct 22)

Plasmid

#55199

Purpose

CFP reporter along with FF4 miRNA binding sites and 28 nt sequences

Depositor

Timothy Lu

Article

Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

Insert

ECFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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NHA-TNGW1

Plasmid

#36437

Depositor

Edward Chan

Article

Yao et al Nucleic Acids Res. 2011 Apr;39(7):2534-4

Insert

TNRC6A (TNRC6A Human)

Tags

NHA

Expression

Mammalian

Mutation

Full length (aa1-1962)

Available Since

June 1, 2012

Availability

Academic Institutions and Nonprofits only