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pBW2589_pCAG-PV1-PYL-L1-VCre-270C-NLS-BGHpA

Plasmid

#108786

Purpose

Expresses aa270 to C-terminus of VCre recombinase fused to PYL CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

VCre recombinase fragment aa270 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

NLS and PYL

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW811_pCAG-PV8-F14-GFP-T2A-mCherry-BGHpA

Plasmid

#87587

Purpose

PV8 for 3-input, 2-output Full Adder

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

EGFP, mCherry

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-pCAG-Floxed-FlpO-2A-H2BV5c.o.,rev.-WPRE

Plasmid

#234894

Purpose

CAG-driven, Cre-dependent FlpO recombinase for H2B-V5

Depositor

Silvia Arber

Article

Falasconi et al Nature. 2025 May 28. doi: 10.1038/s41586

Insert

Floxed-FlpO-2A-H2BV5

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-eCas9-T2A-EGFP-U6-gRNA-no ITR and f1 ori

Plasmid

#234724

Purpose

All-in-one CRISPR/Cas9 vector with high-fidelity eSpCas9 expression in neurons. The plasmid lacks AAV2 ITR and f1 ori elements, enabling more efficient transfection and expression.

Depositor

Kirill Martemyanov

Article

Zhao et al Nat Commun. 2025 Apr 22;16(1):3768. doi:

Type

Empty backbone

Use

CRISPR

Tags

T2A-GFP

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN-Luciferase-P2A-H2A-mCherry (JDW 1129)

Plasmid

#229823

Purpose

A CAGGS driven luciferase reporter followed by a P2A cleavage peptide and an H2A mCherry cassette for nuclear labeling.

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

Luciferase-P2A-H2A-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS puro -GFP-delta73-82 REX1-IRES-mCherry

Plasmid

#232246

Purpose

Mammalian expression of delta73-82 REX1-GFP-IRES-mCherry

Depositor

Francisco Bustos

Article

Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

Insert

delta73-82 REX1-GFP-IRES-mCherry (Zfp42 Mouse)

Use

Tags

AcGFP1

Expression

Mammalian

Mutation

delta73-82

Promoter

Available since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS puro human-GFP-RLIM-IRES-mCherry

Plasmid

#232243

Purpose

Mammalian expression of RLIM-GFP-IRES-mCherry

Depositor

Francisco Bustos

Article

Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

Insert

RLIM-GFP-IRES-mCherry (RLIM Human)

Use

Tags

AcGFP1

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS puro GFP-REX1[degron]-IRES-mCherry

Plasmid

#232240

Purpose

Mammalian expression of REX1[degron]-GFP-IRES-mCherry

Depositor

Francisco Bustos

Article

Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

Insert

REX1[degron]-GFP-IRES-mCherry (Zfp42 Mouse)

Use

Tags

AcGFP1

Expression

Mammalian

Mutation

REX1 (aa 70-85)

Promoter

Available since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-GST-ATG13 (460-517)-L476/L479D

Plasmid

#210845

Purpose

Mammalian expression of ATG13 (460-517) mutant

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

ATG13 (ATG13 Human)

Use

Tags

Expression

Mammalian

Mutation

L476/L479D

Promoter

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-GST-ATG13 (450-517)-A452D/F453D

Plasmid

#210851

Purpose

Mammalian expression of ATG13 (450-517) mutant

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

ATG13 (ATG13 Human)

Use

Tags

Expression

Mammalian

Mutation

A452D/F453D

Promoter

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-GST-ATG13 (460-517)-F509/F512D

Plasmid

#210847

Purpose

Mammalian expression of ATG13 (460-517) mutant

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

ATG13 (ATG13 Human)

Use

Tags

Expression

Mammalian

Mutation

F509/F512D

Promoter

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-FIP200 (1-640)-L324D/R543D/R548D-TSF

Plasmid

#210842

Purpose

Mammalian expression of FIP200 NTD mutant

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

FIP200 (RB1CC1 Human)

Use

Tags

Expression

Mammalian

Mutation

L324D/R543D/R548D

Promoter

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-GST-ATG13 (460-517)-L495/L499D

Plasmid

#210846

Purpose

Mammalian expression of ATG13 (460-517) mutant

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

ATG13 (ATG13 Human)

Use

Tags

Expression

Mammalian

Mutation

L495/L499D

Promoter

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-GST-ATG13 (460-517)-F466D/F470D

Plasmid

#210844

Purpose

Mammalian expression of ATG13 (460-517) mutant

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

ATG13 (ATG13 Human)

Use

Tags

Expression

Mammalian

Mutation

F466D/F470D

Promoter

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-AaCas12b(Q119F/E475R/E758R)-T2A-EGFP

Plasmid

#188272

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Chen et al Innovation (Camb). 2022 May 26;3(4):1002

Insert

AaCas12b(Q119F/E475R/E758R)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

none

Promoter

CAG

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-PlmCasX(T26R/K610R/K808R)-T2A-EGFP

Plasmid

#188274

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Chen et al Innovation (Camb). 2022 May 26;3(4):1002

Insert

PlmCasX(T26R/K610R/K808R)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

none

Promoter

CAG

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-lox-inv[Talpha1-iCre-pA]-lox-mOrange2-C1

Plasmid

#196877

Purpose

Neuron-specific expression of mOrange2 reporter

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

mOrange2

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Quimeric CAG

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-rox-inv[Talpha1-iCre-pA]-rox-LynGFP

Plasmid

#196874

Purpose

Neuron-specific expression of LynGFP reporter. Used in combination with Talpha1-Dre-pA plasmids

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

LynGFP

Use

Cre/Lox; Dre/rox

Tags

Expression

Mammalian

Mutation

Promoter

Quimeric CAG

Available since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-DIO-inv[roxSTOProx-Lyn-GFP-bGH]

Plasmid

#196884

Purpose

Dual Cre/Dre-dependent expression of LynGFP. Used as Cre-Dre cotransfection reporter

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

roxSTOProx-Lyn-GFP-bGH

Use

Cre/Lox; Dre/rox

Tags

Expression

Mammalian

Mutation

Promoter

Quimeric CAG

Available since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-GST-ATG9 (828-839)-ATG101 (1-198)

Plasmid

#188073

Purpose

To make ATG101/ATG13-HORMA and ATG9 C-terminal tail fusion complex for expression in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG9 and ATG101 (ATG9A, ATG101 Human)

Use

Tags

GST tag

Expression

Mammalian

Mutation

codon-optimized ATG9 (828-839) and codon-optimize…

Promoter

CMV

Available since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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