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p1192-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIC3Nme_FLAG_NLS

Plasmid

#129530

Purpose

Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIC3Nme

Depositor

Erik Sontheimer

Article

Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

Inserts

codon-optimized AcrIIC3Nme
sgRNA_Rosa26

Use

AAV

Tags

FLAG/NLS

Promoter

U6 and cytomegalovirus-enhancer chicken β-actin p…

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pOP/SSAT

Plasmid

#140291

Purpose

Expresses human SSAT in bacterial cells

Depositor

Saverio Bettuzzi, Gaetano Marverti

Article

Marverti et al Carcinogenesis. 2005 Oct;26(10):1677-86.

Insert

SSAT1 (SAT1 Human)

Expression

Bacterial

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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HOXB4 (human) pSG5

Plasmid

#8527

Depositor

Corey Largman

Article

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB4 (HOXB4 Human)

Use

In vitro transcriptioin/translation

Expression

Mammalian

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pOPINK-OTUD3 (OTU+UBA, aa 52-275)

Plasmid

#61411

Purpose

Expresses human OTUD3 (OTU+UBA domains) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD3 (OTUD3 Human)

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-51 and aa 276-398.

Promoter

T7

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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RnBarr1-mVenus

Plasmid

#137790

Purpose

Visualization of beta-arrestin1

Depositor

Dorus Gadella

Article

Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

Insert

Rat beta-arrestin 1 (Arrb1 Rat)

Expression

Mammalian

Promoter

CMV

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pET14b-PITPalpha

Plasmid

#58256

Purpose

bacterial expression of human PITP alpha

Depositor

Shamshad Cockcroft

Article

Hara et al J Biol Chem. 1997 Jun 6;272(23):14908-13

Insert

PITP alpha (PITPNA Human)

Tags

HIS

Expression

Bacterial

Promoter

T7

Available Since

July 28, 2014

Availability

Academic Institutions and Nonprofits only
p1194-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIA4_FLAG_NLS

Plasmid

#129532

Purpose

Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIA4

Depositor

Erik Sontheimer

Article

Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

Inserts

codon-optimized AcrIIA4
sgRNA_Rosa26

Use

AAV

Tags

FLAG/NLS

Promoter

U6 and cytomegalovirus-enhancer chicken β-actin p…

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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Th-tdTomato

Plasmid

#159460

Purpose

Targeting vector to TH gene locus for induction of P2A-fused tdTomato, with floxed PGK-puroR drug resistant cassette

Depositor

Yuejun Chen

Article

Xiong et al Cell Stem Cell. 2020 Sep 16. pii: S1934-

Insert

P2A-tdTomato

Use

Cre/Lox

Tags

P2A

Expression

Mammalian

Promoter

Endogenous TH

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pmiRGLO Dual-Luciferase-PIK3R1-3'UTR

Plasmid

#182252

Purpose

Human PIK3R1 3` UTR region containing 1x binding site for miR-150 cloned downstream of luciferase reporter gene

Depositor

Silvia Monticelli

Article

Chirichella et al PLoS Biol. 2022 Feb 10;20(2):e3001538. d

Insert

3' UTR region of PIK3R1 gene

Use

Luciferase

Expression

Mammalian

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pRc/CMV-Beta(4)Integrin

Plasmid

#16265

Depositor

Arthur Mercurio

Article

Clarke et al J Biol Chem. 1995 Sep 29. 270(39):22673-

Insert

Beta 4 Integrin (ITGB4 Human)

Expression

Mammalian

Mutation

lacked the 70- and 7-amino acid splice variants a…

Available Since

March 6, 2008

Availability

Academic Institutions and Nonprofits only
pCIG-Flag-Sox9-IRES-nls-GFP

Plasmid

#44281

Depositor

Martin Cheung

Article

Liu et al Proc Natl Acad Sci U S A. 2013 Feb 19;11

Insert

Flag-Sox9 (SOX9 Chicken)

Tags

Flag

Expression

Mammalian

Promoter

CMV-IE enhancer and Chicken beta-actin promoter

Available Since

Aug. 19, 2013

Availability

Academic Institutions and Nonprofits only
pOPINK-VCPIP (OTU, aa 25-561)

Plasmid

#61583

Purpose

Expresses human VCPIP (OTU domain) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

VCPIP (VCPIP1 Human)

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-24 and aa 562-1222.

Promoter

T7

Available Since

Dec. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-mGrn-myc

Plasmid

#178944

Purpose

Construct for expressing murine progranulin (Grn) in AAV

Depositor

Erik Roberson

Article

Arrant et al Brain. 2017 May 1;140(5):1447-1465. doi:

Insert

Grn (Grn Mouse)

Use

AAV

Tags

Myc

Promoter

CBA

Available Since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Beta(4)Integrin-DeltaCYT

Plasmid

#16264

Depositor

Arthur Mercurio

Article

Clarke et al J Biol Chem. 1995 Sep 29. 270(39):22673-

Insert

Beta 4 Integrin (ITGB4 Human)

Expression

Mammalian

Mutation

truncated cytoplasmic tail introduced a stop cod…

Available Since

March 6, 2008

Availability

Academic Institutions and Nonprofits only
pOPINK-OTUD1 (OTU, aa 287-437)

Plasmid

#61407

Purpose

Expresses human OTUD1 (OTU domain) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD1 (OTUD1 Human)

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-286 and aa 438-481.

Promoter

T7

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pOPINK-OTUB2 (full-length, aa 1-234)

Plasmid

#61421

Purpose

Expresses human OTUB2 (full-length) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUB2 (Ubiquitin thioesterase OTUB2) (OTUB2 Human)

Tags

His6-GST-3C

Expression

Bacterial

Promoter

T7

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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1121 pcDNA3 GFP FKHR H215R

Plasmid

#9027

Depositor

William Sellers

Article

Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

Insert

FKHR H215R (FOXO1 Human)

Tags

GFP

Expression

Mammalian

Mutation

H215R

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
pX459-HypaCas9-TUBA1B_sgRNA

Plasmid

#183889

Purpose

pX459V2.0-HypaCas9 plasmid with TUBA1B sgRNA for N-terminal tagging of alpha-tubulin in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

TUBA1B sgRNA spacer (TUBA1B Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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RnBarr1-mTurquoise2

Plasmid

#137789

Purpose

Visualization of beta-arrestin1

Depositor

Dorus Gadella

Article

Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

Insert

Rat beta-arrestin 1 (Arrb1 Rat)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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1120 pBabe puroL HA FKHR H215R

Plasmid

#9026

Depositor

William Sellers

Article

Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

Insert

FKHR H215R (FOXO1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

H215R

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only