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171,035 results

Showing: 6401 - 6410 of 171035 results

  1. Nluc CD63

    Plasmid
    #242528
    Purpose
    This plasmid can be used to quantify CD63 by luminescence signal upon providing substrate, in particular in the secretome, especially in the extracellular vesicles of cells expressing this construct.
    Depositor
    Clotilde Thery
    Article
    Grisard et al J Extracell Vesicles. 2022 Jul;11(7):e12
    Insert
    CD63 (CD63 Human)
    Use
    Luciferase
    Tags
    Nanoluciferase
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    242528-plasmid-map-sequence-id-500759

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  2. RED

    Plasmid
    #139835
    Purpose
    Expresses RNase H1-EGFP- dCas9 fusion protein in human cells
    Depositor
    Karim Mekhail
    Article
    Abraham et al Nature. 2020 Sep;585(7824):298-302. doi:
    Insert
    RNase H1-EGFP-dCas9 (RNASEH1 RNase H1 (H. sapiens), dCas9 (S. pyogenes))
    Expression
    Mammalian
    Mutation
    dCas9 protein contains two mutations: D10A and H8…
    Promoter
    tet-inducible CMV
    Available Since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    139835-plasmid-map-sequence-id-310615

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  4. qTAG-AAVS1-Ef1a-Puro-mScarlet

    Plasmid
    #227274
    Purpose
    AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing mScarlet. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    AAVS1 Homology Arms flanking a 2A-Puro-EF1a-mScarlet cassette (AAVS1 Synthetic)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227274-plasmid-map-sequence-id-452035

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  5. pEf1a-hifiDn29-dCas9-P2A-GFP

    Plasmid
    #247158
    Purpose
    Mammalian expression of a human codon optimized engineered hifiDn29 recombinase fused to dCas9 and gRNA expression cassette for targeting attH1 with H1-g3
    Depositor
    Patrick Hsu
    Article
    Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038
    Insert
    hifiDn29-dCas9-P2A-GFP
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    M6I/E70G/A224P/G227V/Q332K/N341K/L393P/D503N/I98V…
    Promoter
    Ef1a
    Available Since
    Nov. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    247158-plasmid-map-sequence-id-491688

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Showing: 6401 - 6410 of 171035 results