9,088 results

Showing: 1281 - 1300 of 9088 results

Results per page:

Sort results by:

pCW57-GFP-2A-MCS

Plasmid

#71783

Purpose

All-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo GFP using the P2A self-cleaving peptide.

Depositor

Adam Karpf

Article

Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral; Doxycycline inducible; p2a self cleav…

Expression

Mammalian

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pUAS:Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2

Plasmid

#74009

Purpose

Tissue-specific knock-out in zebrafish, Cas9 and GFP driven by a UAS promoter

Depositor

Filippo Del Bene

Article

Di Donato et al Genome Res. 2016 Mar 8.

Inserts

5x UAS
Cas9
T2A
GFP
U6 promoter

Use

CRISPR

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-EFS-SpCas9

Plasmid

#104588

Purpose

AAV vector expressing Myc-tagged SpCas9 under the EFS promoter

Depositor

Ryohei Yasuda

Article

Nishiyama et al Neuron. 2017 Oct 18. pii: S0896-6273(17)

Has Service

AAV8

Insert

Streptococcus pyogenes Cas9

Use

AAV and CRISPR

Tags

Myc

Expression

Mammalian

Promoter

EFS

Available Since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMRX-IP-GFP-LC3-RFP-LC3ΔG

Plasmid

#84572

Purpose

Expresses GFP-LC3-RFP-LC3ΔG in mammalian cells to measure autophagic flux

Depositor

Noboru Mizushima

Article

Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Use

Retroviral

Tags

EGFP and mRFP1

Expression

Mammalian

Available Since

Jan. 20, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pEG BacMam

Plasmid

#160451

Purpose

vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

Depositor

Eric Gouaux

Article

Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

Type

Empty backbone

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
PARP1 FL

Plasmid

#169815

Purpose

Expresses codon optimised full length His tagged PARP1 in bacterial cells

Depositor

David Neuhaus

Article

Ogden et al Nucleic Acids Res. 2021 Feb 26;49(4):226

Insert

Poly[ADP-ribose] polymerase 1 (PARP1 Human, Synthetic, Codon optimised)

Tags

MKHHHHHHMKQ

Expression

Bacterial

Mutation

Valine 762 mutated to an alanine

Promoter

T7 promoter

Available Since

Aug. 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST_FLAG-HUWE1

Plasmid

#187120

Purpose

mammalian cell expression of FLAG-HUWE1

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Tags

1xFLAG

Expression

Mammalian

Available Since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-GFP

Plasmid

#11150

Purpose

Mammalian expression vector for expression of GFP (CAG promoter)

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

Has Service

Cloning Grade DNA

Insert

EGFP from Aequorea victoria

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pCSC-ISL1-T2A-LHX3

Plasmid

#90215

Purpose

To convert human skin fibroblasts into induced motor neurons (hiMN) in combination with NGN2, Sox11, FGF2 and two small molecules, forskolin and dorsomorphin.

Depositor

Chun-Li Zhang

Article

Liu et al Cell Rep. 2016 Jan 5;14(1):115-28. doi:

Insert

ISL1-T2A-LHX3 (ISL1, LHX3 Human)

Use

Lentiviral

Promoter

CMV

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2+HyPer7-NLS

Plasmid

#136468

Purpose

Mammalian expression of nucleus targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

Depositor

Vsevolod Belousov

Article

Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

Insert

HyPer7

Tags

Triple nuclear localization signal of SV40 (simia…

Expression

Mammalian

Promoter

CMV, SP6

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-dCasRx-ALKBH5-PURO

Plasmid

#175582

Purpose

Expresses dCasRx-ALKBH5 in mammalian cell

Depositor

Qi Xie

Article

Xia et al Nucleic Acids Res. 2021 Jul 21;49(13):73

Insert

dCasRx-ALKBH5

Use

CRISPR and Lentiviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

RfxCas13d R239A/H244A/R858A/H863A

Promoter

pMSCV

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-Cre:GFP

Plasmid

#13776

Purpose

Mammalian expression of Cre recombinase fused to GFP

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

Cre-GFP fusion protein

Use

Cre/Lox

Tags

GFP

Expression

Mammalian

Promoter

CAG

Available Since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDH-EF1a-eFFly-mCherry

Plasmid

#104833

Purpose

3rd generation lentiviral vector. eFFly luciferase for in vivo Imaging, mCherry for fluorescent detection

Depositor

Irmela Jeremias

Article

Ebinger et al Cancer Cell. 2016 Dec 12;30(6):849-862.

Inserts

eFFly
mCherry

Use

Lentiviral and Luciferase

Expression

Mammalian

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCas9-sgAAVS1-1

Plasmid

#129726

Purpose

Expressing SpCas9 and sgAAVS1-1

Depositor

Elina Ikonen

Article

Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

Insert

SpCas9 and sgAAVS1-1

Use

CRISPR

Tags

P2A-Puro

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-Rab5a-7

Plasmid

#55126

Purpose

Localization: Ras GTPase, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

Rab5a

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-Egr1

Plasmid

#11729

Depositor

Eileen Adamson

Article

Yu et al Mol Cell. 2004 Jul 2. 15(1):83-94.

Insert

Egr-1 (Egr1 Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
p2attPC

Plasmid

#51547

Purpose

DICE donor plasmid without neural transcription factors.

Depositor

Michele Calos

Article

Zhu et al Nucleic Acids Res. 2013 Dec 4.

Insert

mCherry

Expression

Mammalian

Available Since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL2-2xCLEAR-luciferase

Plasmid

#81120

Purpose

Reporter activity of the CLEAR network

Depositor

Sung Hee Baek

Article

Shin et al Nature. 2016 Jun 15. doi: 10.1038/nature

Insert

2xCLEAR

Use

Luciferase

Tags

luciferase

Promoter

2xCLEAR

Available Since

Dec. 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pShuttle-FEN1hWT

Plasmid

#35027

Depositor

Sheila Stewart

Article

Saharia et al J Biol Chem. 2010 Aug 27;285(35):27057-6

Insert

FEN1 (FEN1 Human)

Use

Adenoviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only
GFP- SEC61B

Plasmid

#121159

Purpose

Mammalian expression of GFP tagged SEC61B, used as a general ER marker

Depositor

Christine Mayr

Article

Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

Insert

SEC61B (SEC61B Human)

Expression

Mammalian

Available Since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge