We narrowed to 4,562 results for: TIL
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Plasmid#121761PurposepMVP L3-L2 entry plasmid, contains 6x His epitope tag + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest in lentivirus vectors.DepositorInsert6x His epitope tag + WPRE
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KO901: pMVP (L5-L4) Blast-P2A
Plasmid#121715PurposepMVP L5-L4 entry plasmid, contains Blast-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term Blast selection marker linked by P2A to gene of interest.DepositorInsertBlast-P2A (open)
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KP101: pMVP (L5-L4) Puro-P2A
Plasmid#121716PurposepMVP L5-L4 entry plasmid, contains Puro-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term Puro selection marker linked by P2A to gene of interest.DepositorInsertPuro-P2A (open)
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KP001: pMVP (L5-L4) Neo-P2A
Plasmid#121717PurposepMVP L5-L4 entry plasmid, contains Neo-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term Neo selection marker linked by P2A to gene of interest.DepositorInsertNeo-P2A (open)
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KK802: pMVP (L5-L4) Zeo-P2A
Plasmid#121719PurposepMVP L5-L4 entry plasmid, contains Zeo-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term Zeo selection marker linked by P2A to gene of interest.DepositorInsertZeo-P2A (open)
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IG901: pMVP (L5-L4) FLAG epitope tag
Plasmid#121701PurposepMVP L5-L4 entry plasmid, contains FLAG epitope tag for 4-component MultiSite Gateway Pro assembly. Allows N-term epitope tag fusion to gene of interest.DepositorInsertFLAG epitope tag (open)
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IH101: pMVP (L5-L4) OLLAS epitope tag
Plasmid#121703PurposepMVP L5-L4 entry plasmid, contains OLLAS epitope tag for 4-component MultiSite Gateway Pro assembly. Allows N-term epitope tag fusion to gene of interest.DepositorInsertOLLAS epitope tag (open)
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IX104: pMVP (L5-L4) myc epitope tag
Plasmid#121704PurposepMVP L5-L4 entry plasmid, contains myc epitope tag for 4-component MultiSite Gateway Pro assembly. Allows N-term epitope tag fusion to gene of interest.DepositorInsertmyc epitope tag (open)
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KG703: pMVP (L5-L4) 6x His epitope tag
Plasmid#121706PurposepMVP L5-L4 entry plasmid, contains 6x His epitope tag for 4-component MultiSite Gateway Pro assembly. Allows N-term epitope tag fusion to gene of interest.DepositorInsert6x His epitope tag (open)
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IO801: pMVP (L5-L4) 3x FLAG epitope tag
Plasmid#121702PurposepMVP L5-L4 entry plasmid, contains 3x FLAG epitope tag for 4-component MultiSite Gateway Pro assembly. Allows N-term epitope tag fusion to gene of interest.DepositorInsert3x FLAG epitope tag (open)
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVR2
Plasmid#84719PurposeE. coli RecA promoter followed by stretch with no A's followed by riboswitch for single-round transcriptionDepositorInsertsE. coli RecA promoter
stretch lacking A's followed by 5' UTR of queC gene
ExpressionBacterialMutation12 nt stretch lacking A's inserted before 5&…Available SinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC176
Plasmid#118891Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal SNAP, cleavable with TEVDepositorInsertNcoI-HIS6-MBP-3C-SNAP-TEV-NotI-ccdB-AscI-stop-HindIII cassette
TagsHIS6-MBP, cleavable with 3C protease; SNAP, cleav…ExpressionInsectPromoterpolHAvailable SinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCSD_SpCas9MT2-NLS-3xHA-NLS-SaCas9WT-2xNLS
Plasmid#107321PurposeExpresses SpCas9 (R1333S) fused to SaCas9 in mammalian cellsDepositorInsertSpCas9-SaCas9 fusion
UseCRISPRTagsNLSExpressionMammalianMutationSpCas9 (R1333S) is fused to SaCas9PromoterCMV IE94Available SinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
St1Cas9_LMD-9_sgRNA_pUC19
Plasmid#110627PurposeU6-driven sgRNA expression plasmid for Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9).DepositorInsertEmpty St1Cas9 LMD-9 sgRNA backbone
UseCRISPR and Synthetic BiologyTagsNoneExpressionMammalianPromoterhU6Available SinceMay 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO/FLAG-hPMR1(2H)-Tev-Bio
Plasmid#80325PurposeExpresses a catalytically inactive form of human PMR1 endonuclease with N-terminal FLAG and C-terminal biotinylation tagsDepositorInsertPXDNL-003 (PXDNL Human)
TagsBiotinExpressionMammalianMutationH170A and H256APromoterCMVAvailable SinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
AP54-5
Plasmid#65599Purposeco-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 geneDepositorInsertsgRNA for APs12
UseCRISPRExpressionWormPromoterU6Available SinceJune 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR SFFV tdTomato L5-L2
Plasmid#62155PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a SFFV promoter and tdTomato module. Compatible with MultiSite Gateway cloningDepositorInserttdTomato
UseMule gateway entry vectorExpressionMammalianPromoterSFFVAvailable SinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR CMV iRFP L3-L2
Plasmid#62159PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and iRFP module. Compatible with MultiSite Gateway cloningDepositorInsertiRFP
UseMule gateway entry vectorExpressionMammalianPromoterCMVAvailable SinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR SV40 PuroR L5-L2
Plasmid#62165PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and Puromycin module. Compatible with MultiSite Gateway cloningDepositorInsertPuromycin
UseMule gateway entry vectorExpressionMammalianPromoterSV40Available SinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR U6 n.s shRNA L1-L4
Plasmid#62136PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and a non-silencing (scrambled) shRNA module. Compatible with MultiSite Gateway cloningDepositorInsertnon-silencing (scrambled) shRNA
UseRNAi; Mule gateway entry vectorExpressionMammalianPromoterU6Available SinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR U6 n.s shRNA R4-R3
Plasmid#62137PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and a non-silencing (scrambled) shRNA module. Compatible with MultiSite Gateway cloningDepositorInsertnon-silencing (scrambled) shRNA
UseRNAi; Mule gateway entry vectorExpressionMammalianPromoterU6Available SinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR U6 n.s shRNA L5-L2
Plasmid#62139PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and a non-silencing (scrambled) shRNA module. Compatible with MultiSite Gateway cloningDepositorInsertnon-silencing (scrambled) shRNA
UseRNAi; Mule gateway entry vectorExpressionMammalianPromoterU6Available SinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR SV40 eGFP L3-L2
Plasmid#62145PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and EGFP module. Compatible with MultiSite Gateway cloningDepositorInserteGFP
UseMule gateway entry vectorExpressionMammalianPromoterSV40Available SinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR 7SK-miR-30 R4-R3
Plasmid#62121PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseRNAi; Mule gateway entry vectorExpressionMammalianAvailable SinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR 7SK-miR-30 L1-R5
Plasmid#62118PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseRNAi; Mule gateway entry vectorExpressionMammalianAvailable SinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
AP56-6
Plasmid#65593Purposeco-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 geneDepositorInsertsgRNA for APa13
UseCRISPRExpressionWormPromoterU6Available SinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
AP56-5
Plasmid#65591Purposeco-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 geneDepositorInsertsgRNA for APa13
UseCRISPRExpressionWormPromoterU6Available SinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
AP56-8
Plasmid#65596Purposeco-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 geneDepositorInsertsgRNA for APa13
UseCRISPRExpressionWormPromoterU6Available SinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
AP56-7
Plasmid#65594Purposeco-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 geneDepositorInsertsgRNA for APa13
UseCRISPRExpressionWormPromoterU6Available SinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
AP54-3
Plasmid#65597Purposeco-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 geneDepositorInsertsgRNA for APs12
UseCRISPRExpressionWormPromoterU6Available SinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR CMV iRFP R4-R3
Plasmid#62158PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and iRFP module. Compatible with MultiSite Gateway cloningDepositorInsertiRFP
UseMule gateway entry vectorExpressionMammalianPromoterCMVAvailable SinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 PAK5 V593I
Plasmid#60539PurposeDonor vector for human Pak5 V593I mutantDepositorAvailable SinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 PAK5 P50L
Plasmid#60533PurposeDonor vector for human Pak5 P50L mutantDepositorAvailable SinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 PAK5 P76T
Plasmid#60534PurposeDonor vector for human Pak5 P76T mutantDepositorAvailable SinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 PAK5 S312C
Plasmid#60535PurposeDonor vector for human Pak5 S312C mutantDepositorAvailable SinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 PAK5 V463L
Plasmid#60536PurposeDonor vector for human Pak5 V463L mutantDepositorAvailable SinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR SV40 eGFP L5-L2
Plasmid#62144PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and EGFP module. Compatible with MultiSite Gateway cloningDepositorInserteGFP
UseMule gateway entry vectorExpressionMammalianPromoterSV40Available SinceJan. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSC-hXRCC1-K176R-IntE2
Plasmid#52277PurposeBacterial expression of human XRCC1-K176R C-terminally fused to GyrA intein, SUMO-E2 and GSTDepositorInsertXRCC1 (XRCC1 Human)
TagsGyrA-intein-Ubc9-GSTExpressionBacterialMutationK176R, SUMO acceptor site mutantPromoterT7Available SinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSC-hXRCC1-K176R-PreE2
Plasmid#52279PurposeBacterial expression of human XRCC1-K176R C-terminally fused to SUMO-E2 and GSTDepositorInsertXRCC1 (XRCC1 Human)
TagsUbc9-GSTExpressionBacterialMutationK176R, SUMO acceptor site mutantPromoterT7Available SinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFSW Doc2alpha D291N mutant
Plasmid#128813PurposeTo make virus expressing Doc2alpha D291 mutantDepositorInsertDoc2alpha (Doc2a Rat)
UseLentiviralTagsIRES-EGFPMutationD291NPromoterhSyn (human synapsin I promoter)AvailabilityAcademic Institutions and Nonprofits only -
pFSW Doc2beta D303N mutant
Plasmid#128815PurposeTo make virus expressing Doc2beta D303N mutantDepositorInsertDoc2beta (Doc2b Rat)
UseLentiviralTagsIRES-EGFPMutationD303NPromoterhSyn (human synapsin I promoter)AvailabilityAcademic Institutions and Nonprofits only -
pFSW Doc2alpha Halotag
Plasmid#128817PurposeTo make virus expressing Doc2alpha HalotagDepositorInsertDoc2alpha (Doc2a Rat)
UseLentiviralTagsHaloTag-IRES-EGFPPromoterhSyn (human synapsin I promoter)AvailabilityAcademic Institutions and Nonprofits only -
pFSW Doc2beta Halotag
Plasmid#128818PurposeTo make virus expressing Doc2beta HalotagDepositorInsertDoc2beta (Doc2b Rat)
UseLentiviralTagsHaloTag-IRES-EGFPPromoterhSyn (human synapsin I promoter)AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PKAsub(T/A)-YPet-NES
Plasmid#138205PurposeEncodes negative-control substrate fragment of FRET-based bimolecular PKA activity reporter (BimAKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.DepositorInsertPKAsub(T/A)-YPet-NES
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PKAsub(T/A)-RLuc8N-NES
Plasmid#138212PurposeEncodes negative-control substrate fragment of luminescence-based bimolecular PKA activity reporter (LumAKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.DepositorInsertPKAsub(T/A)-RLuc8N-NES
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
mCherry
Plasmid#176016PurposeMammalian expression of mCherry. Parton lab clone DFQDepositorInsertmCherry
ExpressionMammalianAvailable SinceNov. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP
Plasmid#176015PurposeMammalian expression of EGFP. Parton lab clone DFSDepositorInsertEGFP
ExpressionMammalianAvailable SinceNov. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti-Dual-iCas9-CMV
Plasmid#167500PurposeExpression of dual inducible Cas9 with CMV promoterDepositorInsertCas9
UseCRISPR, Lentiviral, and LuciferaseTagsFLAGExpressionMammalianPromoterCMVAvailable SinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDisCoTune
Plasmid#173713PurposePromotes expression of disulfide-rich protein in E. coli T7 expression system. The plasmid encodes expression of the folding factors Erv1p and hPDI controlled by the Ptac promoter.DepositorInsertsExpressionBacterialAvailable SinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR-PGK-scFv GCN4-sfGFP-VP64
Plasmid#122133PurposeExpress a scFv which binds to GCN4, sfGFP and VP64 cassette from the PGK promoterDepositorInsertscFv GCN4-sfGFP-VP64
UseCRISPR and LentiviralExpressionMammalianPromoterPGKAvailable SinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only