We narrowed to 22,079 results for: his
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Plasmid#236395PurposeExpresses His6-MBP-TEV-Rgs14 in E. coliDepositorInsertRattus norvegicus regulator of G-protein signaling 14 (Rgs14 Rat)
UseTagshexahistidine (H6) tag and maltose-binding protei…ExpressionBacterialMutationPromoterAvailable sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
GFP-mZO-1(9-1665)-Myc-His
Plasmid#236530PurposeFor expression of ZO-1 (9-1665 aa, mouse)-Myc-His alpha- in mammalian cellsDepositorInsertTJP1 (Tjp1 Mouse)
UseTagsGFP and Myc-HisExpressionMammalianMutationWTPromoterAvailable sinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
GFP-mZO-1(9-1745)-Myc-His
Plasmid#236529PurposeFor expression of ZO-1 (9-1745 aa, mouse)-Myc-His alpha+ in mammalian cellsDepositorInsertTJP1 (Tjp1 Mouse)
UseTagsGFP and Myc-HisExpressionMammalianMutationWTPromoterAvailable sinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
T7-RBS-6xHis-SUMO-SynCas.v10 bacteria
Plasmid#208594PurposeProduction and purification of catalytically active SynCas.v10DepositorInsertSynCas.v10
UseTagsExpressionBacterialMutationPromoterT7Available sinceApril 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
T7-RBS-6xHis-SUMO-dSynCas.v10 bacteria
Plasmid#208595PurposeProduction and purification of catalytically inactive SynCas.v10DepositorInsertdSynCas.v10
UseTagsExpressionBacterialMutationPromoterT7Available sinceApril 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
T7-RBS-6xHis-SUMO-SynCas.v12 bacteria
Plasmid#208596PurposeProduction and purification of catalytically active SynCas.v12DepositorInsertSynCas.v12
UseTagsExpressionBacterialMutationPromoterT7Available sinceApril 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
T7-RBS-6xHis-SUMO-dSynCas.v12 bacteria
Plasmid#208597PurposeProduction and purification of catalytically inactive SynCas.v12DepositorInsertdSynCas.v12
UseTagsExpressionBacterialMutationPromoterT7Available sinceApril 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-His(6)-TEV-AtPCO4-2(wt)
Plasmid#191802PurposeFull length wt AtPCO4-2 with Nt-His tagDepositorInsertAtPCO4-2
UseTags6xHis, TEV cleavage siteExpressionBacterialMutationPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD80ENSF26-Pct3-(+)-Eldh1(nHis6)(GpDSM19378)
Plasmid#233874PurposeExpresses His-tagged Gordonibacter pamelaeae DSM 19378 (+)-Eldh1 with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purificationDepositorInsert(+)-Eldh1 (GPA_25810 Gordonibacter pamelaeae DSM 19378)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
BD-pGEX5X3-BD-SARS-CoV-2-S-tail-His1271Lys
Plasmid#218456PurposeBacterial expression plasmid for the SARS-CoV-2 cytosolic tail (His1271Lys substitution) with an N-terminal GST tagDepositorInsertCytosolic tail of SARS-CoV-2 spike carrying His1271Lys substitution and an N-terminal GST-tag (S SARS-CoV-2)
UseTagsGSTExpressionBacterialMutationHis1271Lys substitutionPromoterAvailable sinceMarch 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
10xHis-3C-BRD4(42-459) BD1-BD2
Plasmid#233886PurposepRSF-DUET1 for protein overexpression of human BRD4DepositorInsertBRD4 (BRD4 Human)
UseTags10x his 3CExpressionBacterialMutationPromoterT7Available sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD80ENSF25-Pct3-(–)-Eddh(nHis6)(G3C)
Plasmid#233873PurposeExpresses His-tagged Gordonibacter pamelaeae 3C (–)-Eddh with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purificationDepositorInsert(–)-Eddh
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXD80ENSF27-Pct3-(+)-Eldh2(nHis6)(GpDSM19378)
Plasmid#233875PurposeExpresses His-tagged Gordonibacter pamelaeae DSM 19378 (+)-Eldh2 with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purificationDepositorInsert(+)-Eldh2 (GPA_25800 Gordonibacter pamelaeae DSM 19378)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET/His-GST-Rab1a(Q70L)
Plasmid#232911PurposeE. coli ExpressionDepositorInsertRAB1A (RAB1A Human)
UseTagsHis-GSTExpressionBacterialMutationQ70LPromoterAvailable sinceMarch 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-FLAG-APEX2-NES-HIS3
Plasmid#229230PurposeTemplate plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeastDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
CLIPf-KIF5A-SNAPf-HisTag-FLAG
Plasmid#200789PurposeMammalian expression of CLIPf-KIF5A-SNAPf-HisTag-FLAGDepositorInsertKIF5A (Kif5a Mouse)
UseTagsCLIPf and SNAPf, HisTag, FLAGExpressionMammalianMutationPromoterCMV & T7Available sinceDec. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
CLIPf-KIF5B-SNAPf-HisTag-FLAG
Plasmid#200799PurposeMammalian expression of CLIPf-KIF5B-SNAPf-HisTag-FLAGDepositorInsertKIF5B (KIF5B Human)
UseTagsCLIPf and SNAPf, HisTag, FLAGExpressionMammalianMutationPromoterCMV & T7Available sinceDec. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET30-10His-FKBP(f36v)-SNAP
Plasmid#200282PurposeFor recombinant expression in E.coli (Rosetta) of a 10His-tag - FKBP(f36v) - SNAPtag fusion proteinDepositorInsertFKBP-SNAPtag
UseTagsHis-tagExpressionBacterialMutationPromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-T7-OmpA-MNaseB-6xHis
Plasmid#214809PurposeExpression of 6xhis-tagged micrococcal nuclease B (MNaseB)DepositorInsertOmpA-Micrococcal nuclease B
UseTags6xhisExpressionBacterialMutationPromoterT7 promoterAvailable sinceNov. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFa6-TID-FRB-3HA-HIS3MX6
Plasmid#228021PurposeFor TurboID-FRB integration; yeast BioID with HIS3MX6 markerDepositorInsertTurboID-FRB
UseTagsHA tagExpressionYeastMutationPromoterAvailable sinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFa6-TID-FKBP-3HA-HIS3MX6
Plasmid#228025PurposeTo integrate TID-FKBP fusion at yeast promoter; yeast BioID with HIS3MX6 markerDepositorInsertTurboID-FKBP
UseTagsHA tagExpressionYeastMutationPromoterAvailable sinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416-HIS3p-TID-FRB-HA3
Plasmid#228004PurposeExpresses TurboID-FRB fusion from HIS3 promoter; yeast BioIDDepositorInsertTurboID-FRB
UseTagsHA tagExpressionYeastMutationPromoterHIS3Available sinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416-HIS3p-TID-FKBP-HA3
Plasmid#228006PurposeExpresses TurboID-FKBP fusion from HIS3 promoter; yeast BioIDDepositorInsertTurboID-FKBP
UseTagsHA tagExpressionYeastMutationPromoterHIS3Available sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPICZ A-SLC25A46[I349D]-His (SB256)
Plasmid#227605PurposeInducible expression of His-tagged SLC25A46 with I349D mutation in Pichia pastorisDepositorInsertSLC25A46 (SLC25A46 Human)
UseTags10xHisExpressionYeastMutationI349DPromoterAOX1Available sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM315 pACYC-His6-Rpn10[ΔUIM]
Plasmid#226348PurposeExpression of yeast Rpn10 with the UIM deletedDepositorInsertrpn10 (RPN10 Budding Yeast)
UseTagsHisExpressionBacterialMutationdelta UIMPromoterAvailable sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
1GFP-His-GFP-TEV-cs-ATG3_Y209A
Plasmid#197473PurposeBacterial Expression of human ATG3 Y209ADepositorInsertATG3 (ATG3 Human)
UseTagsHis-GFPExpressionBacterialMutationY209APromoterAvailable sinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
1GFP-His-GFP-TEV-cs-ATG3_Y210A
Plasmid#197474PurposeBacterial Expression of human ATG3 Y210ADepositorInsertATG3 (ATG3 Human)
UseTagsHis-GFPExpressionBacterialMutationY210APromoterAvailable sinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
1GFP-His-GFP-TEV-cs-ATG3_T244A
Plasmid#197475PurposeBacterial Expression of human ATG3 T244ADepositorInsertATG3 (ATG3 Human)
UseTagsHis-GFPExpressionBacterialMutationT244APromoterAvailable sinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
1GFP-His-GFP-TEV-cs-ATG3_H262A
Plasmid#197476PurposeBacterial Expression of human ATG3 H262ADepositorInsertATG3 (ATG3 Human)
UseTagsHis-GFPExpressionBacterialMutationH262APromoterAvailable sinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
1GFP-His-GFP-TEV-cs-ATG3_R265A
Plasmid#197477PurposeBacterial Expression of human ATG3 R265ADepositorInsertATG3 (ATG3 Human)
UseTagsHis-GFPExpressionBacterialMutationR265APromoterAvailable sinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
1GFP-His-GFP-TEV-cs-ATG3_H266A
Plasmid#197478PurposeBacterial Expression of human ATG3 H266ADepositorInsertATG3 (ATG3 Human)
UseTagsHis-GFPExpressionBacterialMutationH266APromoterAvailable sinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
1GFP-His-GFP-TEV-cs-ATG3_H262Q
Plasmid#197479PurposeBacterial Expression of human ATG3 H262QDepositorInsertATG3 (ATG3 Human)
UseTagsHis-GFPExpressionBacterialMutationH262QPromoterAvailable sinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
1GFP-His-GFP-TEV-cs-ATG3_H266Q
Plasmid#197480PurposeBacterial Expression of human ATG3 H266QDepositorInsertATG3 (ATG3 Human)
UseTagsHis-GFPExpressionBacterialMutationH266QPromoterAvailable sinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
1GFP-His-GFP-TEV-cs-ATG3_C264S
Plasmid#197481PurposeBacterial Expression of human ATG3 C264SDepositorInsertATG3 (ATG3 Human)
UseTagsHis-GFPExpressionBacterialMutationC264SPromoterAvailable sinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
1GFP-His-GFP-TEV-cs-ATG3_C264A
Plasmid#197482PurposeBacterial Expression of human ATG3 C264ADepositorInsertATG3 (ATG3 Human)
UseTagsHis-GFPExpressionBacterialMutationC264APromoterAvailable sinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG8Ha-His-EWSR1-359-549
Plasmid#195868PurposeExpresses the RRM-RG2-ZnF (359-549) of EWSDepositorInsertEWS 359-549 (EWSR1 Human)
UseTagsHis8-linker-TEVExpressionBacterialMutationPromoterT7Available sinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SdrG-B2-ELP-HIS-ybbR
Plasmid#169134PurposeE. coli expression of SdrG N2, N3 and B2 domains containing ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.DepositorInsertStaphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
UseTags3xELP linker, 6x Histag, and ybbr tagExpressionBacterialMutationPromoterAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-VHH-ddFLN4-HIS-ybbR
Plasmid#169137PurposeE. coli expression of VHH domain containing ybbR tag and ddFLN4 fingerprint domain. This construct was designed for AFM-SMFS measurements.DepositorInsertVHH
UseTags6x His tag, ddFLN4 tag, and ybbr tagExpressionBacterialMutationPromoterAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a- mCherry-FIVAR-HIS-ybbR
Plasmid#169138PurposeE. coli expression of mCherry domain containing ybbR tag and FIVAR fingerprint domain. This construct was designed for AFM-SMFS measurements.DepositorInsertmCherry
UseTags6x Histag, FIVAR tag, and ybbr tagExpressionBacterialMutationPromoterAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SdrG-B1-ELP-HIS-ybbR
Plasmid#169133PurposeE. coli expression of SdrG N2, N3 and B1 domains containing ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.DepositorInsertStaphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
UseTags3xELP linker, 6x Histag, and ybbr tagExpressionBacterialMutationPromoterAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPPI4-Strep-SNAP-CD80-His10
Plasmid#223579PurposeProtein purification of human CD80 with Twin-Strep, SNAP and 10xHis tags from mammalian cellsDepositorInsertCD80 (CD80 Human)
UseTagsHis10 and Twin-Strep, SNAPExpressionMammalianMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPPI4-Strep-SNAP-CD86-His10
Plasmid#223570PurposeProtein purification of human CD86 with Twin-Strep, SNAP and 10xHis tags from mammalian cellsDepositorInsertCD86 (CD86 Human)
UseTagsHis6 and Twin-Strep, SNAPExpressionMammalianMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HTB His-TEV-rDPP9
Plasmid#217326PurposePurification of rat DPP9 from insect cellsDepositorInsertDPP9
UseTagsHis-TEVExpressionInsectMutationPromoterpolyhedrinAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF1b-His8-TEV-PI31-1-151
Plasmid#202593PurposeBacterial expression of HsPI31-1-151DepositorInsertPI31 (PSMF1 Human)
UseTags8xHis, TEVExpressionBacterialMutationaa 1-151 onlyPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2-HsProm1s1(W795R)-TwinStrep-His
Plasmid#210817PurposeMammalian overexpression vector for C-terminally TwinStrep and His tagged human Prom1s1 (W795R)DepositorInsertProminin 1 (PROM1 Human)
UseTagsTwinStrep, 10xHisExpressionMammalianMutationChanged Trp-795 to ArgPromoterCMVAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-GAL4-PARP1-CAT-L713F
Plasmid#175949PurposeBacterial expression of a fusion of yeast GAL4 DNA binding domain and human PARP1 CAT domain containing L713F gain-of-function mutationDepositorInsertGAL4 DNA binding domain (GAL4 Budding Yeast)
UseTags6xHis tagExpressionBacterialMutationL713F, V762A for the 'PARP1-CAT-L713F' …PromoterT7Available sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
His-GST-ATG13 (363-517)
Plasmid#222823PurposeBacteria expression of His-GST -ATG13 C-terminalDepositorInsertATG13 (ATG13 Human)
UseTags6His-GSTExpressionBacterialMutationPromoterT7Available sinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-His10-Smt3-ccdB-SNAP
Plasmid#166148PurposeGateway destination vector for generation of N-terminal His10-Smt3 and C-terminal SNAP tagged proteins in E. coli.DepositorTypeEmpty backboneUseTagsHis10-Smt3 and SNAPExpressionBacterialMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-His10-Smt3-DDX5(kf)-SNAP
Plasmid#166149PurposeExpression of N. furzeri DDX5 in E. coliDepositorInsertDDX5
UseTagsHis10-Smt3 and SNAPExpressionBacterialMutationPromoterT7Available sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-His10-Smt3-DDX5ΔPrD(kf)-SNAP
Plasmid#166150PurposeExpression of N. furzeri DDX5ΔPrD(1-535) mutant in E. coliDepositorInsertDDX5ΔPrD(1-535)
UseTagsHis10-Smt3 and SNAPExpressionBacterialMutationΔPrD(1-535)PromoterT7Available sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only