We narrowed to 17,355 results for: URE

1. pGEX6P1-HA-MEK1 CBM

   Plasmid

   #53163

   Purpose

   Expresses GST-HA tagged human MEK1 M187AH188AM230AH239A from bacterial expression vector

   Depositor

   Christopher Counter

   Article

   Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Insert

   MEK1 (MAP2K1 Human)
   

   Use

   Tags

   GST and HA

   Expression

   Bacterial

   Mutation

   M187A/H188A/M230A/H239A

   Promoter

   Available Since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pGEX6P1-HA-MEK5 CBM-E

   Plasmid

   #53168

   Purpose

   Expresses GST-HA tagged human MEK5 L280AH281AM323AQ332A from bacterial expression vector

   Depositor

   Christopher Counter

   Article

   Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Insert

   MEK5 (MAP2K5 Human)
   

   Use

   Tags

   GST and HA

   Expression

   Bacterial

   Mutation

   L280A/H281A/M323A/Q332A

   Promoter

   Available Since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pKMW281_dSpRY

   Plasmid

   #244822

   Purpose

   Mammalian expression of catalytically inactive SpRY Cas9 (dSpRY)

   Depositor

   Jennifer Doudna

   Article

   Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

   Insert

   dSpRY Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG-SV40 NLS and Nucleoplasmin NLS

   Expression

   Mammalian

   Mutation

   D10A/A61R/H840A/L1111R/D1135L/S1136W/G1218K/E1219…

   Promoter

   CAG

   Available Since

   Sept. 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKMW122_SpG-Cas9_U6-entry

   Plasmid

   #244819

   Purpose

   Mammalian expression of SpG Cas9 with Golden Gate-compatible sgRNA spacer cassette

   Depositor

   Jennifer Doudna

   Article

   Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

   Insert

   SpG Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG-SV40 NLS and Nucleoplasmin NLS

   Expression

   Mammalian

   Mutation

   D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

   Promoter

   CAG, U6

   Available Since

   Sept. 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHS73_10xHis_MBP_TEV_SpRY-Cas9_2xNLS

   Plasmid

   #244835

   Purpose

   Bacterial expression of SpRY Cas9 with 2xSV40 nuclear localization sequence for protein purification

   Depositor

   Jennifer Doudna

   Article

   Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

   Insert

   SpRY Cas9
   

   Use

   CRISPR

   Tags

   10xHis-MBP and 2xSV40 NLS

   Expression

   Bacterial

   Mutation

   A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1…

   Promoter

   T7

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. Palpha2

   Plasmid

   #204441

   Purpose

   Mammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.

   Depositor

   Keir Neuman

   Article

   Nyenhuis et al Nature. 2023 Aug 23. doi: 10.1038/s41586

   Insert

   hOpa1 isoform 1 (OPA1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   W771A K772A K773A R774A W775A L776A Y777A W778A K…

   Promoter

   CMV

   Available Since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET21a Sa-iPGM-bio

   Plasmid

   #179523

   Purpose

   Expresses S. aureus iPGM containing a C-terminal biotinylation site and His tag in E. coli

   Depositor

   James Inglese

   Article

   van Neer et al ACS Chem Biol. 2022 Aug 19;17(8):2284-22

   Insert

   S. aureus iPGM bio-6His
   

   Use

   Tags

   His tag and biotinylation sequence

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. sgRNA BRCA2 S3070F

   Plasmid

   #139325

   Purpose

   Plasmid expressing a sgRNA to introduce BRCA2 S3070F using base editing

   Depositor

   Alberto Ciccia

   Article

   Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

   Insert

   sgRNA to insert BRCA2 S3070F using base editing
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   May 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. sgRNA BRCA1 S1363L

   Plasmid

   #139329

   Purpose

   Plasmid expressing a sgRNA to introduce BRCA1 S1363L using base editing

   Depositor

   Alberto Ciccia

   Article

   Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

   Insert

   sgRNA to insert BRCA1 S1363L using base editing
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   May 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. sgRNA BRCA1 E1754K

    Plasmid

    #139330

    Purpose

    Plasmid expressing a sgRNA to introduce BRCA1 E1754K using base editing

    Depositor

    Alberto Ciccia

    Article

    Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

    Insert

    sgRNA to insert BRCA1 E1754K using base editing
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    May 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. sgRNA FANCD2 Q223Stop

    Plasmid

    #139332

    Purpose

    Plasmid expressing a sgRNA to introduce FANCD2 Q223Stop using base editing

    Depositor

    Alberto Ciccia

    Article

    Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

    Insert

    sgRNA to insert FANCD2 Q223Stop using base editing
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    May 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-GABARα6N-HA

    Plasmid

    #128111

    Purpose

    Express HA tagged GABARα6 N terminal domain

    Depositor

    Huda Zoghbi

    Article

    Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

    Insert

    Gabra6 (Gabra6 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1/NDUFS2 _2-33/myc-His

    Plasmid

    #127492

    Purpose

    Expresses myc-tagged _MTS NDUFS2 in mammalian cells

    Depositor

    Shashi Jain, Dieter Wolf

    Article

    Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

    Insert

    NDUFS2 _2-33 (NDUFS2 Human)
    

    Use

    Tags

    myc-His

    Expression

    Mammalian

    Mutation

    NDUFS2 _2-33

    Promoter

    CMV

    Available Since

    July 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1/NDUFS2 _2-78/myc-His

    Plasmid

    #127493

    Purpose

    Expresses myc-tagged _2-78 NDUFS2 in mammalian cells

    Depositor

    Shashi Jain, Dieter Wolf

    Article

    Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

    Insert

    NDUFS2 _2-78 (NDUFS2 Human)
    

    Use

    Tags

    myc-His

    Expression

    Mammalian

    Mutation

    NDUFS2 _2-78

    Promoter

    CMV

    Available Since

    July 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJSC115 - Bacterial expression plasmid for SpCas9-HF1, HNH FRET variant

    Plasmid

    #101210

    Purpose

    Bacterial expression plasmid for SpCas9-HF1, HNH FRET variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant C80S/C574S/S355C/S867C/N497A/R661A/Q695A/Q926A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    C80S, C574S, S355C, S867C, N497A, R661A, Q695A an…

    Promoter

    T7

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJSC038 - Bacterial expression plasmid for SpCas9∆REC3, HNH FRET variant

    Plasmid

    #101203

    Purpose

    Bacterial expression plasmid for SpCas9∆REC3, HNH FRET variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant C80S/C574S/S355C/S867C/M1–N497,GGS,V713–D1368
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    C80S, C574S, S355C, S867C, M1-N497, GGS, V713-D13…

    Promoter

    T7

    Available Since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJSC131 - Bacterial expression plasmid for SpCas9-HF1, REC3 FRET variant

    Plasmid

    #101212

    Purpose

    Bacterial expression plasmid for SpCas9-HF1, REC3 FRET variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant C80S/C574S/S701C/S960C/N497A/R661A/Q695A/Q926A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    C80S, C574S, S701C, S960C, N497A, R661A, Q695A an…

    Promoter

    T7

    Available Since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. BPK4393 - human expression plasmid for SpCas9 Cluster 5 + Q926A

    Plasmid

    #101192

    Purpose

    Human expression plasmid for SpCas9 Cluster 5 + Q926A variant: CMV-T7-hSpCas9-Cluster5+Q926A(K918A, V922A, R925A, Q926A)-NLS(SV40)-3xFLAG

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    hSpCas9-Cluster5+Q926A(K918A/V922A/R925A/Q926A)
    

    Use

    Tags

    3x FLAG and NLS (SV40)

    Expression

    Mammalian

    Mutation

    K918A/V922A/R925A/Q926A

    Promoter

    CMV

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. MMW3629 - human expression plasmid for SpCas9 Cluster 3 + Q926A

    Plasmid

    #101188

    Purpose

    Human expression plasmid for SpCas9 Cluster 3 + Q926A variant: CMV-T7-hSpCas9-Cluster3+Q926A(T657A, G658A, W659A, R661A, Q926A)-NLS(SV40)-3xFLAG

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    hSpCas9-Cluster3+Q926A(T657A/G658A/W659A/R661A/Q926A)
    

    Use

    Tags

    3x FLAG and NLS (SV40)

    Expression

    Mammalian

    Mutation

    T657A/G658A/W659A/R661A/Q926A

    Promoter

    CMV

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MMW3759 - human expression plasmid for SpCas9 Cluster 4 + Q926A

    Plasmid

    #101190

    Purpose

    Human expression plasmid for SpCas9 Cluster 4 + Q926A variant: CMV-T7-hSpCas9-Cluster4+Q926A(F491A, M495A, T496A, N497A, Q926A)-NLS(SV40)-3xFLAG

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    hSpCas9-Cluster4+Q926A(F491A/M495A/T496A/N497A/Q926A)
    

    Use

    Tags

    3x FLAG and NLS (SV40)

    Expression

    Mammalian

    Mutation

    F491A/M495A/T496A/N497A/Q926A

    Promoter

    CMV

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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