pAcHELIX-sgRNA_entry (CJT94)
Plasmid
#181783
Purpose
Expresses AcHELIX containing a nicking I-AniI fusion to AcTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
Depositor
Insert
nAniI-AcTnsB, AcTnsC, AcTniQ, AcCas12k
Expression
Bacterial
Mutation
nAniI = K227M, F80K, L232K
Promoter
Lac and J23119
Available Since
March 31, 2022
Availability
Academic Institutions and Nonprofits only
