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PRDM9(KRAB-SSXRD-PR/SET-ZF1-dC)-Cas9

Plasmid

#184464

Purpose

Plasmid encoding PRDM9-Cas9 fusion under CMV promoter

Depositor

Jennifer Doudna

Article

Chen et al Proc Natl Acad Sci U S A. 2022 Dec 6;119

Insert

PRDM9(KRAB-SSXRD-PR/SET-ZF1-dC)-Cas9 (PRDM9 Human)

Use

CRISPR

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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p23-NLS-RfxCas13d-mcherry-NLS-Flag

Plasmid

#165077

Purpose

overexpression of RfxCas13d in human cells

Depositor

Ling-Ling Chen

Article

Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

Insert

RfxCas13d

Expression

Mammalian

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiPGK Blast DEST JNKKTRmRuby2

Plasmid

#59154

Purpose

Lentiviral vector to express JNK KTR mRuby2 under PGK promoter (With Blasticidin Resistance)

Depositor

Markus Covert

Article

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

JNK Kinase Translocation Reporter (MAPK8 Human)

Use

Lentiviral

Tags

mRuby2

Expression

Mammalian

Promoter

PGK

Available Since

Sept. 18, 2014

Availability

Academic Institutions and Nonprofits only

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mRFP-Rab7

Plasmid

#14436

Purpose

Mammalian expression of Rab7 fused to mRFP. Used for localization to the late endosome, and other early endosomal vacuoles.

Depositor

Ari Helenius

Article

Vonderheit et al PLoS Biol. 2005 Jul . 3(7):e233.

Insert

Rab 7 (RAB7A Canis familialis)

Tags

mRFP

Expression

Mammalian

Mutation

3 silent mutations near the C-terminus

Available Since

March 23, 2007

Availability

Academic Institutions and Nonprofits only

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pCAG_smFP HA

Plasmid

#59759

Purpose

encodes multiple copies of an epitope tag HA

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Insert

smFP _HA

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 21, 2014

Availability

Academic Institutions and Nonprofits only

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p415 TEF roGFP2‐Tsa2ΔCR

Plasmid

#83238

Purpose

The genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For cytosolic expression.

Depositor

Tobias Dick

Article

Morgan et al Nat Chem Biol. 2016 Jun;12(6):437-43. do

Insert

roGFP2-Tsa2dCr

Tags

roGFP2 fusion with Tsa2dCr

Expression

Yeast

Promoter

TEF

Available Since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pET21b-Caspase-3

Plasmid

#90087

Purpose

Expresses wild type caspase 3 in bacterial cells

Depositor

Clay Clark

Article

Pop et al Biochemistry. 2001 Nov 27;40(47):14224-3

Insert

Caspase 3 (CASP3 Human)

Tags

His tag

Expression

Bacterial

Mutation

Wild type

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-TDP-43

Plasmid

#190093

Purpose

Expresses TDP-43 in mammalian cells. Note: EGFP and TDP-43 are expressed in different reading frames.

Depositor

Bowen Liu

Article

Liu et al Commun Biol. 2022 Aug 13;5(1):816. doi:

Insert

TAR DNA-Binding Protein 43 (TARDBP Human)

Expression

Mammalian

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pEvolvR-enCas9-PolI5M

Plasmid

#113078

Purpose

Expresses enCas9 fused to PolI5M in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.

Depositor

John Dueber, David Schaffer

Article

Halperin et al Nature. 2018 Aug 1. pii: 10.1038/s41586-

Insert

enCas9-PolI5M

Use

CRISPR

Expression

Bacterial

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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piggyBac-rtTA (4th_Gen)-NFIB-SOX9-IRES-mKate2 (VK_1244)

Plasmid

#209082

Purpose

NFIB and SOX9 mediated astrocye induction

Depositor

Vikram Khurana

Article

Lam et al bioRxiv 2022.11.08.515615

Inserts

NFIB-P2A-SOX9-V5 (NFIB, SOX9 Human)
mKate2

Expression

Mammalian

Promoter

TRE4G

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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