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Plasmid#178954PurposeIt consists of a catalytically active Cas9 nuclease and MS2-SunTag-activators activation complex (ScFv-sfGFP-2xTAD), enabling simultaneous genome editing and gene activation.DepositorInsertzCas9-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-double floxed-Gad65-WPRE-HGHpA
Plasmid#184631PurposeExpresses Gad65, driven by the Ef1a promoter, in a Cre-dependent fashionDepositorInsertGad65 (Gad2 Mouse)
UseAAVTagsExpressionMutationPromoterEF1aAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV/TO SV40 small + Large T (w612-1)
Plasmid#22298DepositorInsertSV40 small + Large T antigen
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMx PylT_GFP 150TAG
Plasmid#140016PurposePlasmid with 4xMxPylT cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integrationDepositorInsertsfGFP
UseTagsExpressionMammalianMutation150TAG, K46E (Please see depositor comments below)PromoterEF1Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro U6 sgRNA BfuAI stuffer
Plasmid#50920PurposeU6 driven sgRNA cloning vector where guide sequences are inserted between BfuAI sitesDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLPC-FLAG.Mm.EPC2
Plasmid#184649PurposeRetroviral expression of mouse EPC2DepositorInsertEpc2 (Epc2 Mouse)
UseRetroviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLPC-FLAG.Mm.EPC1
Plasmid#184648PurposeRetroviral expression of mouse EPC1DepositorInsertEPC1 (Epc1 Mouse)
UseRetroviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCIG3_mCherry_Rab5_DN
Plasmid#75111Purposeexpression of mCherry-Rab5 DN and EGFPDepositorInsertRAB5A (RAB5A Human)
UseLentiviralTagsmCherryExpressionMammalianMutationDN S34NPromoterCMVAvailable sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EGFR-G719S
Plasmid#116256PurposeLentiviral expression of EGFR G719SDepositorInsertEGFR (EGFR Human)
UseLentiviralTagsExpressionMutationG719SPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
CMVTO-SEAP-26Sfur-B2AD-tevs-KKMP
Plasmid#179636PurposeTEVP-inducible single TM SEAP RELEASE constructDepositorInsertSEAP RELEASE
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only