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Plasmid#215560PurposeConstitutively expressed sgRNA targeting a desired gene (this case pta), ColE1 origin, KanRDepositorInsertgRNA
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119Available sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLG023 (dsr2)
Plasmid#157901PurposeExpresses the dsr2 defense systemDepositorInsertdsr2 (SIR2)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG022 (dsr1)
Plasmid#157900PurposeExpresses the dsr1 defense systemDepositorInsertdsr1 (SIR2-DUF4020)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT AID*-GFP osTIR1
Plasmid#182514PurposeC-SWAT compatible vector for C-terminal tagging with AID*-GFP for auxin inducible gene knockdown. osTIR1 is flanked by SIR4 homology arms for integration.DepositorInsertAID*
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1-p19-T7-EGFP100
Plasmid#46309DepositorInsertsp19
EGFP100 DNA-1
linker
EGFP100 DNA-2
UsePro-sirnaTags6XHis and GSTExpressionBacterialMutationPromoterAvailable sinceJan. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1-p19-T7
Plasmid#46306DepositorInsertsp19
DNA-1 site
linker
DNA-2 site
UsePro-sirnaTags6XHis and GSTExpressionBacterialMutationPromoterTacAvailable sinceJuly 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pWZLblast-RalA WT(RalA 402i-resistant)
Plasmid#11124DepositorInsertRalA WT (RALA Human)
UseRetroviralTagsExpressionMammalianMutationresistant to siRNA 402iPromoterAvailable sinceMay 25, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1-p19-T7-EGFPFL
Plasmid#46310DepositorInsertsp19
EGFPFL DNA-1
linker
EGFPFL DNA-2
UsePro-sirnaTags6XHis and GSTExpressionBacterialMutationPromoterAvailable sinceJan. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA-HIRAmut-polyA
Plasmid#59782PurposeThis Hira gene contains silent mutations in siRNA-targeted sequences (see Inoue and Zhang, 2014 NSMB).DepositorInsertHIRA
UseTagsExpressionMammalianMutationChange from 'GATCGAAGTTTG' to 'GAc…PromoterCMV and T7Available sinceSept. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSuperRetro-puro-RalB 45i
Plasmid#11122DepositorUseRNAi and RetroviralTagsExpressionMammalianMutationN/APromoterAvailable sinceMay 25, 2006AvailabilityAcademic Institutions and Nonprofits only -
UBE2G2 shRNA
Plasmid#201813PurposesiRNADepositorInsertUBE2G2 (UBE2G2 Human)
UseRNAiTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT IAA17
Plasmid#193319PurposeC-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin-Inducible Degron). ARF16(PB1)-P2A-osTIR1 has SIR4 homology arms for genome integration.DepositorInsertIAA17
UseTagsIAA17ExpressionYeastMutationPromoterAvailable sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT AID*-6MYC osTIR1
Plasmid#193320PurposeC-SWAT compatible vector for C-terminal tagging with AID*-6MYC for auxin induced gene knockdown. osTIR1 is flanked by SIR4 homology arms for integration into the genome.DepositorInsertAID*
UseTags6MYCExpressionYeastMutationPromoterAvailable sinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT AID*-6FLAG osTIR1
Plasmid#182515PurposeC-SWAT compatible vector for C-terminal tagging with AID*-6FLAG for auxin induced gene knockdown. osTIR1 is flanked by SIR4 homology arms for integration into the genome.DepositorInsertAID*
UseTags6FLAGExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT IAA17-6FLAG
Plasmid#182516PurposeC-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin-Inducible Degron) and 6FLAG. ARF16(PB1)-P2A-osTIR1 has SIR4 homology arms for genome integration.DepositorInsertIAA17
UseTags6FLAGExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT IAA17-3HA
Plasmid#182517PurposeC-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin inducible degron) and 3HA. ARF16(PB1)-P2A-osTIR1 with SIR4 homology arms for genome integration.DepositorInsertIAA17
UseTags3HAExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT IAA17-3MYC
Plasmid#182518PurposeC-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin inducible degron) and 3MYC. ARF16(PB1)-P2A-osTIR1 with SIR4 homology arms for genome integration.DepositorInsertIAA17
UseTags3MYCExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQlox66R
Plasmid#135657PurposeClostridial vector, encoding a targetron carrying a lox66 site in the reverse (antisense) orientation. Retarget intron to insert lox66 onto genome at desired site.DepositorInsertIntron containing lox site
UseCre/LoxTagsExpressionBacterialMutationPromoterpFerAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQlox71R
Plasmid#135656PurposeClostridial vector, encoding a targetron carrying a lox71 site in the reverse (antisense) orientation. Retarget intron to insert lox71 onto genome at desired site.DepositorInsertIntron containing lox site
UseCre/LoxTagsExpressionBacterialMutationPromoterpFerAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only