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pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(R635A)

Plasmid

#231384

Purpose

Express mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cells

Depositor

David Bartel

Article

Mohamed et al Cell Rep. 2025 Jan 28;44(1):115166. doi:

Insert

Human Argonaute2 (AGO2 Human, Synthetic)

Use

Tags

3xFLAG-SUMO_Eu1

Expression

Mammalian

Mutation

R635A

Promoter

CMV

Available since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(EI 5xE)

Plasmid

#231385

Purpose

Express mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cells

Depositor

David Bartel

Article

Mohamed et al Cell Rep. 2025 Jan 28;44(1):115166. doi:

Insert

Human Argonaute2 (AGO2 Human, Synthetic)

Use

Tags

3xFLAG-SUMO_Eu1

Expression

Mammalian

Mutation

S824E, S828E, T830E, S831E, S834E

Promoter

CMV

Available since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(D669A)

Plasmid

#231371

Purpose

Express catalytically dead mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cells

Depositor

David Bartel

Article

Mohamed et al Cell Rep. 2025 Jan 28;44(1):115166. doi:

Insert

Human Argonaute2 (AGO2 Human, Synthetic)

Use

Tags

3xFLAG-SUMO_Eu1

Expression

Mammalian

Mutation

D669A

Promoter

CMV

Available since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FRB:GSlinker:SSD

Plasmid

#228535

Purpose

FRB protein in fusion with a bipartite SSD degron, consisting of fragments of human Skp1 and SOCS2 proteins, through a flexible glycine-serine linker.

Depositor

Roman Jerala

Article

Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80

Insert

FRB:GSlinker:SSD (SKP1, SOCS2 Human, Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-CLIPf

Plasmid

#200787

Purpose

For the mammalian expression of CLIPf-HisTag-FLAG fusion protein.

Depositor

Alison Twelvetrees

Article

Smith et al bioRxiv 2024.12.20.629623

Insert

CLIPf-HisTag-FLAG

Use

Tags

CLIPf

Expression

Mammalian

Mutation

Promoter

CMV & T7

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-SNAPf

Plasmid

#200788

Purpose

For the mammalian expression of SNAPf-HisTag-FLAG fusion protein.

Depositor

Alison Twelvetrees

Article

Smith et al bioRxiv 2024.12.20.629623

Insert

SNAPf-HisTag-FLAG

Use

Tags

SNAPf

Expression

Mammalian

Mutation

Promoter

CMV & T7

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Plagl2

Plasmid

#228061

Purpose

transiently express mouse Plagl2

Depositor

Kenneth Chen

Article

Fraire et al bioRxiv [Preprint]. 2024 Apr 30:2024.04.

Insert

Plagl2 (Plagl2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_λHA-GFPopt

Plasmid

#210575

Purpose

Expresses lambdaHA-optimal GFP

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

LambdaHA-GFP optimal

Use

Tags

lambda HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_λHA-GFPnonopt

Plasmid

#210576

Purpose

Expresses lambdaHA-nonoptimal GFP

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

LambdaHA-GFP nonoptimal

Use

Tags

lambda HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_SM-FLuc0-pA

Plasmid

#210583

Purpose

Expresses SM(FLAG)-nonoptimal Firefly luciferase- pA

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Spaghetti monster (FLAG)-nonoptimal FLuc

Use

Luciferase

Tags

Spaghetti monster (FLAG)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_SM-FLuc100-pA

Plasmid

#210584

Purpose

Expresses SM(FLAG)-optimal Firefly luciferase - pA

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Spaghetti monster (FLAG)-optimal FLuc

Use

Luciferase

Tags

Spaghetti monster (FLAG)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-Regnase-3 WT

Plasmid

#222655

Purpose

Mammalian expression vector to express mouse Regnase-3 WT

Depositor

Silvia Monticelli

Article

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12c (Zc3h12c Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ar2

Plasmid

#225220

Purpose

Expression androgen receptor 2 in mammalian cells

Depositor

Deshou Wang

Article

Xiao et al Commun Biol. 2024 Sep 16;7(1):1154. doi:

Insert

androgen receptor 2

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) Rheb(WT)

Plasmid

#224289

Purpose

Cell transfection and expression of Rheb

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

Rheb (RHEB Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-DmPGC

Plasmid

#220309

Purpose

red-light-activated guanylyl cyclase DmPGC

Depositor

Andreas Moeglich

Article

Xu et al J Mol Biol. 2024 Mar 1;436(5):168257. do

Insert

DmPGC

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 28, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_NAP1-eGFP

Plasmid

#216837

Purpose

For mammalian expression of NAP1-GFP (Internal ref. SMC1638)

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

NAP1

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_ALX4-Flag

Plasmid

#215029

Purpose

Mammalian expression of human ALX4

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

ALX homeobox 4 (ALX4 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_V5-TWIST1_NEUROD1loop

Plasmid

#215032

Purpose

Mammalian expression of human TWIST1 (with NEUROD1 loop)

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

twist family bHLH transcription factor 1 (TWIST1 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

TWIST1 bHLH loop (aa134-141, IIPTLPSD) replaced w…

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_V5-TWIST1_NEUROG2loop

Plasmid

#215033

Purpose

Mammalian expression of human TWIST1 (with NEUROG2 loop)

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

twist family bHLH transcription factor 1 (TWIST1 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

TWIST1 bHLH loop (aa137-141, TLPSD) replaced with…

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_V5-TWIST1_HAND2loop

Plasmid

#215034

Purpose

Mammalian expression of human TWIST1 (with HAND2 loop)

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

twist family bHLH transcription factor 1 (TWIST1 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

TWIST1 bHLH loop (aa137-141, TLPSD) replaced with…

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge