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We narrowed to 1,740 results for: plasmid for e coli

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Showing: 1301 - 1320 of 1740 results

  5. pET-6His-MBP-MpAgo

    Plasmid
    #79249
    Purpose
    This plasmid encodes the Argonaute protein from Marinitoga Piezophila and is codon optimized for E. coli expression
    Depositor
    Jennifer Doudna
    Article
    Kaya et al Proc Natl Acad Sci U S A. 2016 Mar 30. p
    Insert
    Marinitoga piezophila Argonaute
    Use
    CRISPR
    Tags
    6XHis-tag + MBP + PreScission Cleavage Site
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    July 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    79249-plasmid-map-sequence-id-151486

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  6. pPP537

    Plasmid
    #158803
    Purpose
    pRSF-Duet1 derived plasmid containing C-terminally 2xSV40 NLS and hexa-histidine tagged CasΦ-2 in MCS1 for expression in E. coli and protein purification
    Depositor
    Jennifer Doudna
    Article
    Pausch et al Science. 2020 Jul 17;369(6501):333-337.
    Insert
    CasPhi-2
    Tags
    2xSV40NLS-6xHis
    Expression
    Bacterial
    Available Since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    158803-plasmid-map-sequence-id-309579

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  13. pEA02

    Plasmid
    #172193
    Purpose
    Suicide vector carrying the site attL of the SSR system of pSAM2. This plasmid is integrated into the genome of Actinobacteria by HR when carrying a DNA fragment identical to a region of the genome.
    Depositor
    Jean-Luc Pernodet
    Article
    Santos et al Microorganisms. 2022 Apr 16;10(4). pii:
    Insert
    attL
    Expression
    Bacterial
    Available Since
    Sept. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    172193-plasmid-map-sequence-id-338644

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  14. pZA16mflon

    Plasmid
    #75439
    Purpose
    Plasmid for arabinose-induced expression of mf-lon. Note that this plasmid contains the original mf-lon cassette that is not codon optimized for expression in E. coli.
    Depositor
    James Collins
    Article
    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.
    Insert
    mf-lon
    Expression
    Bacterial
    Promoter
    pBAD
    Available Since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75439-plasmid-map-sequence-id-147407

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  15. I766T USP7 UBL-12

    Plasmid
    #227232
    Purpose
    Expresses human UBL-12 domain of USP7 with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Tags
    6His
    Expression
    Bacterial
    Mutation
    I766T; aa 535-775
    Promoter
    T7-promoter
    Available Since
    April 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    227232-plasmid-map-sequence-id-468099

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  16. L757P USP7 UBL-12

    Plasmid
    #227231
    Purpose
    Expresses human UBL-12 domain of USP7 with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Tags
    6His
    Expression
    Bacterial
    Mutation
    L757P; aa 535-775
    Promoter
    T7-promoter
    Available Since
    April 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    227231-plasmid-map-sequence-id-468098

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  17. R152C USP7 TRAF

    Plasmid
    #227229
    Purpose
    Expresses human TRAF-like domain of USP7 with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Tags
    6His
    Expression
    Bacterial
    Mutation
    R152C; aa 62-205
    Promoter
    T7-promoter
    Available Since
    Nov. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    227229-plasmid-map-sequence-id-452330

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  18. G392A FL USP7

    Plasmid
    #227217
    Purpose
    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Tags
    6His
    Expression
    Bacterial
    Mutation
    G392A
    Promoter
    T7-promoter
    Available Since
    Nov. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    227217-plasmid-map-sequence-id-452329

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  19. L469R USP7 catalytic

    Plasmid
    #227212
    Purpose
    Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Tags
    6His
    Expression
    Bacterial
    Mutation
    L469R; aa 207-560
    Promoter
    T7-promoter
    Available Since
    Nov. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    227212-plasmid-map-sequence-id-452328

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  20. N418S FL USP7

    Plasmid
    #227215
    Purpose
    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Tags
    6His
    Expression
    Bacterial
    Mutation
    N418S
    Promoter
    T7-promoter
    Available Since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    227215-plasmid-map-sequence-id-449605

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Showing: 1301 - 1320 of 1740 results