We narrowed to 13,583 results for: TIM

1. NeonGreen-C1-ORP2

   Plasmid

   #220069

   Purpose

   overexpresses ORP2 with a neonGreen fluorophore

   Depositor

   Gerry Hammond

   Article

   Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

   Insert

   OSBPL2 (OSBPL2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5/FRT/TO_NES_HaloTag9

   Plasmid

   #175537

   Purpose

   Strict cytosolic expression of HaloTag9 in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   NES-HaloTag9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Promoter

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pnos-Cas9-nos

   Plasmid

   #62208

   Purpose

   Expresses Cas9 under control of nanos promoter and 3'UTR. For germ line restricted genome engineering in Drosophila melanogaster.

   Depositor

   Simon Bullock

   Article

   Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

   Insert

   cas9
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   nanos

   Available since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-HIFR

   Plasmid

   #192946

   Purpose

   Lentiviral production of HIF-1alpha reporter

   Depositor

   Markus Covert

   Article

   Jeknic et al J Biol Chem. 2023 Mar 10:104599. doi: 10

   Insert

   HIF-1alpha reporter
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   mCMV

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFastBac_Dual_SNF2L-opt_p48

   Plasmid

   #102243

   Purpose

   Expression of human codon optimized human SNF2L and pRbAp48 from pFastBac dual

   Depositor

   Joe Landry

   Inserts

   SNF2L (SMARCA1 Human)
   pRbAp48 (RBBP4 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   codon optimized for expression

   Promoter

   Polyhedrin and p10

   Available since

   Nov. 1, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. NeonGreen-C1-ORP6

   Plasmid

   #220072

   Purpose

   overexpresses ORP6 with a neonGreen fluorophore

   Depositor

   Gerry Hammond

   Article

   Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

   Insert

   OSBPL6 (OSBPL6 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTubulin-Electra2

   Plasmid

   #184930

   Purpose

   Tubulin labeling with blue fluorescent protein Electra2

   Depositor

   Kiryl Piatkevich

   Article

   Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

   Insert

   TUBA1B-Electra2
   

   Use

   Tags

   Electra2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Myc-PSF-WT

   Plasmid

   #35183

   Depositor

   Benjamin Blencowe

   Article

   Rosonina et al Mol Cell Biol. 2005 Aug;25(15):6734-46.

   Insert

   PSF (SFPQ Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHT-ads

   Plasmid

   #47382

   Purpose

   Expresses ads under Pgrac promoter (inducible by IPTG) in bacteria

   Depositor

   Heng-Phon Too

   Article

   Zhou et al Biotechnol Bioeng. 2013 Mar 8. doi: 10.1

   Insert

   ads
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Pgrac

   Available since

   Oct. 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTubulin-mTagBFP2

    Plasmid

    #184931

    Purpose

    Tubulin labeling with blue fluorescent protein mTagBFP2

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    TUBA1B-mTagBFP2
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV_AncBE4max_3xHA

    Plasmid

    #112097

    Purpose

    C:G-to-T:A base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

    Insert

    AncBE4max_3xHA
    

    Use

    CRISPR

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1(-)-qmTQ2-ATP

    Plasmid

    #225248

    Purpose

    mammalian expression of qmTQ2-ATP, a cyan fluorescent FLIM biosensor for ATP imaging

    Depositor

    Yasushi Okada

    Article

    Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

    Insert

    qmTQ2-ATP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1(+)-OTI-TCRA_p2_TCRB

    Plasmid

    #131033

    Purpose

    Expresses OT-I TCRA linked to TCRB with the P2A self-cleaving peptide

    Depositor

    Jeff Hammerbacher

    Article

    Aksoy et al bioRxiv

    Inserts

    TCRA (Tcra Mouse)
    TCRB (Tcrb Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/T7

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCHERRY3

    Plasmid

    #24659

    Depositor

    Tanya Parish

    Article

    Carroll et al PLoS One. 2010 . 5(3):e9823.

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 9, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMiniT:SEP-GluA2 TKIT donor

    Plasmid

    #169421

    Purpose

    Donor DNA for TKIT knockin of SEP to GluA2 in mouse

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    Super ecliptic pHluorin (SEP)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. VPS13D^mScarlet

    Plasmid

    #174110

    Purpose

    The insert is a codon optimized DNA sequence encoding VPS13D with an internal mScarlet protein.

    Depositor

    Pietro De Camilli

    Article

    Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212

    Insert

    Codon optimized VPS13D
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    With an internal mScarlet tag in aminoacid positi…

    Promoter

    CMV

    Available since

    Dec. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. DL033:cFos::eGFP-iPAK4

    Plasmid

    #177883

    Purpose

    eGFP-iPAK4 fusion with cFos promoter, cloned into a lentivirus backbone

    Depositor

    Adam Cohen

    Article

    Lin et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    iPAK4
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    cFos

    Available since

    July 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AAV-oChIEF-citrine

    Plasmid

    #50974

    Purpose

    AAV transfer vector with mammalian codon-optimized ChIEF-citrine between AAV2 ITR

    Depositor

    Roger Tsien

    Article

    Lin et al Biophys J. 2009 Mar 4;96(5):1803-14. doi

    Insert

    ChIEF-citrine
    

    Use

    AAV

    Tags

    citrine

    Expression

    Mutation

    ChIEF gene is mammalian codon optimized

    Promoter

    human synapsin promoter

    Available since

    Feb. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPL5071_TEF1*-Cre_URA3

    Plasmid

    #60930

    Purpose

    Constitutive expression vector for Cre-recombinase containing URA3 selection marker.

    Depositor

    Robert Piper

    Article

    MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

    Insert

    Cre
    

    Use

    Tags

    Driven from a mutant TEF1 promoter with 0.16x act…

    Expression

    Yeast

    Mutation

    Promoter

    mutant TEF1

    Available since

    Feb. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pChREBP

    Plasmid

    #39235

    Depositor

    Isabelle Leclerc

    Article

    da Silva Xavier et al J Lipid Res. 2006 Nov;47(11):2482-91. Ep

    Insert

    ChREBP (Mlxipl Mouse)
    

    Use

    Tags

    cMyc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

21. YeNL/pcDNA3

    Plasmid

    #85201

    Purpose

    Yellow color variant of bright luminescent protein

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    Yellow enhanced Nano-lantern
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

22. pcDNA3.1(+)-OTI-TCRB_p2_TCRA

    Plasmid

    #131034

    Purpose

    Expresses OT-I TCRB linked to TCRA with the P2A self-cleaving peptide

    Depositor

    Jeff Hammerbacher

    Article

    Aksoy et al bioRxiv

    Inserts

    TCRB (Tcrb Mouse)
    TCRA (Tcra Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/T7

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. ReNL/pcDNA3

    Plasmid

    #85203

    Purpose

    Red color variant of bright luminescent protein

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    Red enhanced Nano-lantern
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. eMagAF-EGFP-Mito

    Plasmid

    #162243

    Purpose

    Blue-light dependent heterodimerization system

    Depositor

    Pietro De Camilli

    Article

    Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

    Insert

    eMagAF-EGFP-OMP25
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pNLS-TetR-GFP

    Plasmid

    #103838

    Purpose

    Flourescent marker for tetO arrays

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    NLS-TetR (tetR )
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    TetR: A4G (M2V)

    Promoter

    CMV

    Available since

    Dec. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. CeNL_pRSETb

    Plasmid

    #89510

    Purpose

    Cyan color variant of bright luminescent protein for bacterial expression

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    CeNL
    

    Use

    Tags

    His X 6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAJM.011

    Plasmid

    #108529

    Purpose

    TetR + PTet*-YFP Reporter. aTc sensor for use in non-Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    TetR + PTet*-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pFN18A-HaloTag-SpyCatcher

    Plasmid

    #206041

    Purpose

    HaloTag-Spy0128-(Protein of Interest)-Spy0128-SpyCatcher-8xHisTag

    Depositor

    Sergi Garcia-Manyes

    Article

    Tapia-Rojo et al Nat Protoc. 2024 Mar 11. doi: 10.1038/s4

    Type

    Empty backbone

    Use

    Tags

    HaloTag and SpyCatcher-8His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA5/FRT/TO_Lifeact_HaloTag9

    Plasmid

    #175545

    Purpose

    Expression of HaloTag9 at the actin filaments of mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Lifeact-HaloTag9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pFA6a-link-yomKate2-CaURA3

    Plasmid

    #44878

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized mKate2 with CaUra3 selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yomKate2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge